Comparison of conventional and real-time RT-PCR for the quantitation of jun protooncogene mRNA and analysis of junB mRNA expression in synovial membranes and isolated synovial fibroblasts from rheumatoid arthritis patients

Zeitschrift fur Rheumatologie

Volumn 62, Issue 4, 2003, Pages 378-389

  Huber, René ^a   Kunisch, Elke ^a   Glück, Brigitte ^a   Egerer, Renate ^a   Sickinger, Stefan ^b   Kinne, Raimund W ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b HANS KNÖLL INSTITUTE   (Germany)

Author keywords

Conventional RT PCR; JunD junB; Real time RT PCR; Rheumatoid arthritis

Indexed keywords

MATRIX METALLOPROTEINASE; MESSENGER RNA; PROTEIN; PROTEIN FOS; TRANSCRIPTION FACTOR AP 1; TRANSCRIPTION FACTOR JUNB; TRANSCRIPTION FACTOR JUND; UNCLASSIFIED DRUG; PROTEIN C JUN;

ACCURACY; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; FIBROBLAST; GENE EXPRESSION; HUMAN; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; OSTEOARTHRITIS; PATHOGENESIS; PROTO ONCOGENE; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHEUMATOID ARTHRITIS; RNA ANALYSIS; SEQUENCE HOMOLOGY; CHEMISTRY; COMPARATIVE STUDY; EVALUATION; GENE EXPRESSION REGULATION; GENETIC SCREENING; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; ONCOGENE; REFERENCE VALUE; SENSITIVITY AND SPECIFICITY; SYNOVIUM; VALIDATION STUDY;

ARTHRITIS, RHEUMATOID; FIBROBLASTS; GENE EXPRESSION REGULATION; GENES, JUN; GENETIC SCREENING; HUMANS; PROTO-ONCOGENE PROTEINS C-JUN; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; SYNOVIAL MEMBRANE;

EID: 0041377806     PISSN: 03401855     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00393-003-0472-4     Document Type: Article

References (21)

1. Angel P, Karin M (1991) The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim Biophys Acta 1072:129-157
2. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR, Liang MH, Luthra HS (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31:315-324
3. Berger I, Shaul Y (1991) Structure and function of human jun-D. Oncogene 6:561-566
4. Berger I, Shaul Y (1994) The human junD gene is positively and selectively autoregulated. DNA Cell Biol 13:249-255
5. Blaschke V, Reich K, Blaschke S, Zipprich S, Neumann C (2000) Rapid quantitation of proinflammatory and chemoattractant cytokine expression in small tissue samples and monocyte-derived dendritic cells: validation of a new real-time RT-PCR technology. J Immunol Methods 246:79-90
6. Castellazzi M, Spyrou G, La Vista N, Dangy JP, Piu F, Yaniv M, Brun G (1991) Overexpression of cjun, junB, or junD affects cell growth differently. Proc Natl Acad Sci USA 88:8890-8894
7. Firestein GS, Manning AM (1999) Signal transduction and transcription factors in rheumatic disease. Arthritis Rheum 42:609-621
8. Gilliland G, Perrin S, Blanchard K, Bunn HF (1990) Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci USA 87:2725-2729
9. Han Z, Boyle DL, Manning AM, Firestein GS (1998) AP-1 and NF-kappaB regulation in rheumatoid arthritis and murine collagen-induced arthritis. Autoimmunity 28:197-208
10. Hirai SI, Ryseck RP, Mechta F, Bravo R, Yaniv M (1989) Characterization of junD: a new member of the jun proto-oncogene family. EMBO J 8:1433-1439
11. Jung M, Muche JM, Lukowsky A, Jung K, Loening SA (2001) Dimethyl sulfoxide as additive to ready-to-use mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye. Anal Biochem 289:292-295
12. Kinne RW, Boehm S, Iftner T, Aigner T, T, Vornehm S, Weseloh G, Bravo R, Emmrich R, Kroczek RA (1995) Synovial fibroblasts-like cells strongly express jun-B and c-fos proto-oncogenes in rheumatoid- and osteoarthritis. Scand J Rheumatol Suppl 101:121-1215
13. Kovary K, Bravo R (1991) The jun and fos protein families are both required for cell cycle progression in fibroblasts. Mol Cell Biol 11:4466-4472
14. Okazaki S, Ito T, Ui M, Watanabe T, Yoshimatsu K, Iba H (1998) Two proteins translated by alternative usage of initiation codons in mRNA encoding a JunD transcriptional regulator. Biochem Biophys Res Commun 250:347-353
15. Pfarr CM, Mechta F, Spyrou G, Lallemand D, Carillo S, Yaniv M (1994) Mouse JunD negatively regulates fibroblast growth and antagonizes transformation by ras. Cell 76:747-760
16. Rothenburg S, Koch-Nolte F, Rich A, Haag F (2001) A polymorphic dinucleotide repeat in the rat nucleolin gene forms Z-DNA and inhibits promotor activity. Proc Natl Acad Sci USA 98:8985-8990
17. Ryder K, Lanahan A, Perez-Albuerne E, Nathans D (1989) Jun-D: a third member of the jun gene family. Proc Natl Acad Sci USA 86:1500-1503
18. Schutte J, Viallet J, Nau M, Segal S, Fedorko J, Minna J (1989) Jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun. Cell 59:987-997
19. Shaulian E, Karin M (2002) AP-1 as a regulator of cell life and death. Nat Cell Biol 4:131-136
20. Wakisaka S, Suzuki N, Saito N, Ochi T, Sakane T (1998) Possible correction of abnormal rheumatoid arthritis synovial cell function by jun D transfection in vitro. Arthritis Rheum 41:470-481
21. Wellmann S, Taube T, Paal K, Graf von Einsiedel H, Geilen W, Seifert G, Eckert C, Henze G, Seeger K (2001) Specific reverse transcription-PCR quantification of vascular endothelial growth factor (VEGF) splice variants by LightCycler technology. Clin Chem 47:654-660