The most abundantly transcribed human cytomegalovirus gene (β2.7) is non-essential for growth in vitro

Journal of General Virology

Volumn 84, Issue 9, 2003, Pages 2511-2516

  McSharry, Brian P ^a   Tomasec, Peter ^a   Neale, M Lynne ^a   Wilkinson, Gavin W G ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; VIRUS PROTEIN; VIRUS RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; GENE DELETION; GENE FUNCTION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC CODE; GENETIC CONSERVATION; GENETIC TRANSCRIPTION; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYADENYLATION; PRIORITY JOURNAL; RNA TRANSLATION; STRAIN DIFFERENCE; VIROGENESIS; VIRUS GENE; VIRUS ISOLATION; VIRUS MUTANT; VIRUS REPLICATION; VIRUS STRAIN;

AMINO ACID SEQUENCE; BASE SEQUENCE; CYTOMEGALOVIRUS; GENE DELETION; GENES, VIRAL; HUMANS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; RNA, MESSENGER; RNA, VIRAL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; VIRUS REPLICATION;

ANIMALIA; CYTOMEGALOVIRUS; DNA VIRUSES; HUMAN HERPESVIRUS 5; RNA VIRUSES;

EID: 0041351951     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/vir.0.19298-0     Document Type: Article

References (28)

1. Akter, P., Cunningham, C., McSharry, B. P. & 8 other authors (2003). Two novel spliced genes in human cytomegalovirus. J Gen Virol 84, 1117-1122.
2. Aono, T., Kondo, K., Miyoshi, H., Tanaka-Taya, K., Kondo, M., Osugi, Y., Hara, J., Okada, S. & Yamanishi, K. (1998). Monitoring of human cytomegalovirus infections in pediatric bone marrow transplant recipients by nucleic acid sequence-based amplification. J Infect Dis 178, 1244-1249.
3. Bergamini, G., Reschke, M., Battista, M. C., Boccuni, M. C., Campanini, F., Ripalti, A. & Landini, M. P. (1998). The major open reading frame of the β2.7 transcript of human cytomegalovirus: in vitro expression of a protein posttranscriptionally regulated by the 5′ region. J Virol 72, 8425-8429.
4. Cha, T. A., Tom, E., Kemble, G. W., Duke, G. M., Mocarski, E. S. & Spaete, R. R. (1996). Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J Virol 70, 78-83.
5. Chee, M. S., Bankier, A. T., Beck, S. & other authors (1990). Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol 154, 125-169.
6. Dargan, D. J., Jamieson, F. E., MacLean, J., Dolan, A., Addison, C. & McGeoch, D. J. (1997). The published DNA sequence of human cytomegalovirus strain AD169 lacks 929 base pairs affecting genes UL42 and UL43. J Virol 71, 9833-9836.
7. Davison, A. J., Dolan, A., Akter, P., Addison, C., Dargan, D. J., Alcendor, D. J., McGeoch, D. J. & Hayward, G. S. (2003). The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J Gen Virol 84, 17-28.
8. Demarchi, J. M. (1981). Human cytomegalovirus DNA: restriction enzyme cleavage maps and map locations for immediate-early, early and late RNAs. Virology 114, 23-38.
9. DeMarchi, J. M. (1984). The physical and transcriptional organization of the human cytomegalovirus genome. Birth Defects Orig Artic Ser 20, 35-47.
10. Gawn, J. M. & Greaves, R. F. (2002). Absence of IE1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression. J Virol 76, 4441-4455.
11. Geballe, A. P., Spaete, R. R. & Mocarski, E. S. (1986). A cis-acting element within the 5′ leader of a cytomegalovirus beta transcript determines kinetic class. Cell 46, 865-872.
12. Goldmacher, V. S., Bartle, L. M., Skaletskaya, A. & 10 other authors (1999). A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2. Proc Natl Acad Sci U S A 96, 12536-12541.
13. Greenaway, P. J. & Wilkinson, G. W. (1987). Nucleotide sequence of the most abundantly transcribed early gene of human cytomegalovirus strain AD169. Virus Res 7, 17-31.
14. Lord, P. C., Rothschild, C. B., DeRose, R. T. & Kilpatrick, B. A. (1989). Human cytomegalovirus RNAs immunoprecipitated by multiple systemic lupus erythematosus antisera. J Gen Virol 70, 2383-2396.
15. Lurain, N. S., Kapell, K. S., Huang, D. D., Short, J. A., Paintsil, J., Winkfield, E., Benedict, C. A., Ware, C. F. & Bremer, J. W. (1999). Human cytomegalovirus UL144 open reading frame: sequence hypervariability in low-passage clinical isolates. J Virol 73, 10040-10050.
16. McDonough, S. H. & Spector, D. H. (1983). Transcription in human fibroblasts permissively infected by human cytomegalovirus strain AD169. Virology 125, 31-46.
17. McDonough, S. H., Staprans, S. I. & Spector, D. H. (1985). Analysis of the major transcripts encoded by the long repeat of human cytomegalovirus strain AD169. J Virol 53, 711-718.
18. Mocarski, E. S., Prichard, M. N., Tan, C. S. & Brown, J. M. (1997). Reassessing the organization of the UL42-UL43 region of the human cytomegalovirus strain AD169 genome. Virology 239, 169-75.
19. Perng, G. C., Jones, C., Ciacci-Zanella, J. & 8 other authors (2000). Virus-induced neuronal apoptosis blocked by the herpes simplex virus latency-associated transcript. Science 287, 1500-1503.
20. Phillips, A. J., Tomasec, P., Wang, E. C., Wilkinson, G. W. & Borysiewicz, L. K. (1998). Human cytomegalovirus infection downregulates expression of the cellular aminopeptidases CD10 and CD13. Virology 250, 350-358.
21. Prichard, M. N., Penfold, M. E., Duke, G. M., Spaete, R. R. & Kemble, G. W. (2001). A review of genetic differences between limited and extensively passaged human cytomegalovirus strains. Rev Med Virol 11, 191-200.
22. Sharp, T. V., Schwemmle, M., Jeffrey, I., Laing, K., Mellor, H., Proud, C. G., Hilse, K. & Clemens, M. J. (1993). Comparative analysis of the regulation of the interferon-inducible protein kinase PKR by Epstein-Barr virus RNAs EBER-1 and EBER-2 and adenovirus VAI RNA. Nucleic Acids Res 21, 4483-4490.
23. Spaete, R. R. & Mocarski, E. S. (1985). Regulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts. J Virol 56, 135-143.
24. Spaete, R. R. & Mocarski, E. S. (1987). Insertion and deletion mutagenesis of the human cytomegalovirus genome. Proc Natl Acad Sci U S A 84, 7213-7217.
25. Wang, E. C., McSharry, B., Retiere, C., Tomasec, P., Williams, S., Borysiewicz, L. K., Braud, V. M. & Wilkinson, G. W. (2002). UL40-mediated NK evasion during productive infection with human cytomegalovirus. Proc Natl Acad Sci U S A 99, 7570-7575.
26. Wathen, M. W. & Stinski, M. F. (1982). Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early and late times after infection. J Virol 41, 462-477.
27. Wu, T. C., Lee, W. A., Pizzorno, M. C., Au, W. C., Chan, Y. J., Hruban, R. H., Hutchins, G. M. & Hayward, G. S. (1992). Localization of the human cytomegalovirus 2.7-kb major early beta-gene transcripts by RNA in situ hybridization in permissive and nonpermissive infections. Am J Pathol 141, 1247-1254.
28. Zhong, W. & Ganem, D. (1997). Characterization of ribonucleo-protein complexes containing an abundant polyadenylated nuclear RNA encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8). J Virol 71, 1207-1212.