The application of a fibroblast gel contraction model to assess the cytotoxicity of topical antimicrobial agents

Wounds

Volumn 15, Issue 8, 2003, Pages 265-271

  Cochrane, Christine A ^a   Shearwood, Claire ^a   Walker, Michael ^b   Bowler, Phil ^b   Knottenbelt, Derek C ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b UK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT;

ARTICLE; BIOCOMPATIBILITY; CELL IMMORTALIZATION; CONTROLLED STUDY; DEBRIDEMENT; DRUG CYTOTOXICITY; DRUG FORMULATION; EXPERIMENTAL MODEL; FIBROBLAST CULTURE; GRANULATION TISSUE; HUMAN; HUMAN CELL; IN VITRO STUDY; INFLAMMATORY CELL; MEDICAL ASSESSMENT; ULTRAVIOLET IRRADIATION; WOUND CARE; WOUND CONTRACTION; WOUND HEALING;

EID: 0041331650     PISSN: 10447946     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Bell E, Ivarsson B, Merrill C. Production of a tissue-like-structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA 1979;76:1274-8.
2. Cochrane C, Pain R, Knottenbelt DC. In-vitro wound contraction in the horse: Differences between body and limb wounds. WOUNDS 2003;15(6):175-81.
3. Moulin V, Tam BY, Catilloux G, et al. Fetal and human skin fibroblasts display intrinsic differences in contractile ability. J Cell Physiol 2001;188:211-2.
4. Germain L, Jean A, Auger FA, et al. Human wound healing fibroblasts have greater contractile properties than dermal fibroblasts. J Surg Res 1994;57:268-73.
5. Moulin V, Auger FA, Garrel D, et al. Role of wound healing myofibroblasts on reepithelialisation of human skin. Burns 2000;26:3-12.
6. Tomasek JJ, Hay ED, Fujiwara K. Collagen modulates cell shape and cytoskeleton of embryonic corneal and fibroma fibroblasts: Distribution of actin, alpha-actinin, and myosin. Dev Biol 1982;92:107-22.
7. Mauch C, Adelmann GB, Hatamochi A, et al. Collagenase gene expression in fibroblasts is regulated by a three-dimensional contact with collagen. FEBS Lett 1989;250:301-5.
8. Mio T, Adachi Y, Romberger DJ, et al. Regulation of fibroblast proliferation in three-dimensional collagen gel matrix. In-vitro Cell Dev Biol - Anim 1996;32:427-33.
9. Kuhn MA, Smith PD, Hill DP, et al. In-vitro fibroblast populated collagen lattices are not good models of in-vivo clinical wound healing. Wound Repair Regen 2000;8:270-6.
10. Yanase A, Ueda M, Kaneda T, et al. Irradiation effects on wound contraction using a connective tissue model. Ann Plast Surg 1993;30:435-40.
11. Ehrlich HP, Wyler DJ. Fibroblast contraction of collagen lattices in vitro: Inhibition by chronic inflammatory cell mediators. J Cell Physiol 1983;116:341-51.
12. Turner TD, Spyratou O, Schmidt RJ. Biocompatability of wound management products: Standardisation of and determination of cell growth rate in L929 fibroblasts culture. J Pharm Pharmacol 1989;41:775-80.
13. Hidalgo E, Bartolome R, Barroso C, et al. Silver nitrate: Antimicrobial activity related to cytotoxicity in cultured human fibroblasts. Skin Pharmacol Appl Skin Physiol 1998;11:140-51.
14. MacDougall M, Selden JK, Nydegger JR, et al. Immortalised mouse odontoblast cell line M06-G3 application for in-vitro biocompatability testing. Am J Dent 1998;11(Suppl):S11-6.
15. Cochrane CA. Models in vivo of wound healing in the horse and the role of growth factors. Vet Derm 1997;8:259-72.
16. Cochrane CA, Freeman KL, Knottenbelt DC. Effect of growth factors on the characteristics of cells associated with equine wound healing and sarcoid formation. Wound Repair Regen 1996;4:58-65.
17. Kramer SA. Effect of povidone-iodine on wound healing: A review. J Vasc Nurs 1999;17:17-23.
18. Gilchrist BA. Should iodine be reconsidered? Nursing Times 1997;93:70-6.
19. ISO EN 10993-5. Biological Evaluation of Medical Devices. Part 5. Tests for Cytotoxicity, In-Vitro Methods. 1999.
20. Winer BJ. Multifactor experiments having repeated measures on the same elements. In: Winer BJ (ed). Statistical Principles in Experimental Design Second Edition. New York: McGraw-Hill Book Co., 1971;514-99.
21. Van Den Broek PJ, Buys LMF, Van Furth R. Interaction of povidone-iodine compounds, phagocytic cells, and micro-organisms. Antimicrob Agents Chemother 1982;22:593-7.
22. Tafnal FM, Leigh IM, Gibson JR. Comparative toxicity of antimicrobial agents on transformed keratinocytes. J Invest Dermatol 1987;89:316-7.
23. Lineweaver W, Howard R, Soucy D, et al. Topical antimicrobial toxicity. Arch Surg 1985;120:267-70.
24. Doughty D. A rational approach to the use of topical antiseptics. JWOCN 1994;21:224-31.
25. Healthpoint Tissue Management Website: Iodosorb/Iodoflex Prescription Information. Located at http://healthpoint.com/home_tissue.htm. Accessed July 31, 2003.
26. Lawrence JC. The use of iodine as an antiseptic agent. J Wound Care 1998;7:421-5.
27. Burk RI. Povidone-iodine solution in wound treatment. Phys Ther 1998;78:212-8.