Amplification and analysis of T-DNA flanking sequences in transgenic rice

Acta Genetica Sinica

Volumn 28, Issue 4, 2001, Pages 350-351

  Fang, Jin ^a,b   Zhai, Wen Xue ^a   Wang, Wen Ming ^a   Li, Su Wen ^b   Zhu, Li Huang ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b BEIJING NORMAL UNIVERSITY   (China)

Author keywords

Integration; T DNA; Transgenic rice; Vector backbone sequence

Indexed keywords

BACTERIAL DNA; T DNA; T-DNA;

ARTICLE; CHEMISTRY; GENETIC TRANSFORMATION; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RICE; TRANSGENIC PLANT;

BASE SEQUENCE; DNA, BACTERIAL; MOLECULAR SEQUENCE DATA; ORYZA SATIVA; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

EID: 0041075260     PISSN: 03794172     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Berg D, Howe M. In: Mobile DNA. London: Academic Press, 1989.
2. John Z, Patricia Z. Transfer of T-DNA from Agrobacterium to the plant cell. Plant Physiol, 1995, 107: 1041-1047.
3. Zupan J, Muth T R, Draper O, Zambryski P. The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J., 2000, 23(1):11-28.
4. Dong J, Teng W, Buchholz W, Hall T. Agrobacterium-mediated transformation of javanica rices. Molecular Breeding, 1996, 2:267-276.
5. Rashild H, Yokoi S, Toriyama K, Hinata K. Transgenic plant production mediated by Agrobacterium in indica rice. Plant Cell Reports, 1996, 15:727-730.
6. Zhai W, Li X et al. Introduction of a blight resistance gene, Xa21, into Chinese rice varieties through an Agrobacterium-mediated system. Science in China (Series C), 2000, 43(2):361-368.
7. Liu Y G, Robert F W. Thermal saymmetric interlaced PCR: Automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics, 1995, 25:674-681.
8. Liu Y G, Norihiro M, Teruko O, Robert F W. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J., 1995, 8(3):457-463.
9. Makoto T et al. The structures of integration sites in transgenic rice. Plant J., 1997, 11(3):353-361.
10. Tatsuya S, Misa T, Naoki G, Hiromichi M. Structures of transgene loci in transgenic Arabidopsis plants obtained by particle bombardment: Junction regions can bind to nuclear matrices. Gene, 1998, 218:27-35.
11. Reinhold M, Zsuzsanna K, Christiane N et al. T-DNA integration: a mode of illegitimate recombination in plants. EMBO J., 1991, 10(3):697-704.
12. Guus B, Zdena K, Nigel G, Barbara H. Recovery of Agrobacterium tumefaciens T-DNA molecules from whole plants early after transfer. Cell, 1989, 57:847-857.
13. Maria E, Burgund B, Stanton B. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J., 1997, 11(5):945-957.
14. Hiroyuki E et al. Ac as a tool for the functional genomics of rice. Plant J., 1999, 19(5):605-613.
15. Ooms G, Bakker A, Mdendijk L, et al. T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissue of Nicotiana tabacum. Cell, 1982, 30:589-597.
16. Virts E, Gelvin S. Analysis of transfer of tumor-inducing plasmids from Agrobacterium tumefaciens to petunia protoplasts. J. Bact., 1985, 162:1030-1038.
17. Martineau B, Voelker T, Sanders R. On defining T-DNA. Plant Cell, 1994, 6:1032-1033.