Analysis of an insect neuropeptide, Schistocerca gregaria Ion Transport Peptide (ITP), Expressed in insect cell systems

Archives of Insect Biochemistry and Physiology

Volumn 46, Issue 1-2, 2001, Pages 87-94

  Pfeifer, T A ^a   Hegedus, D ^b   Wang, Y J ^a   Zhao, Y ^a   Meredith, J ^a   Brock, H W ^a   Phillips, J E ^a   Grigliatti, T A ^a   Theilmann, D A ^c  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^c SUMMERLAND RESEARCH AND DEVELOPMENT CENTRE   (Canada)

Author keywords

Drosophila Kc1 cells; In vitro expression; Ion transport peptide; Locusts; N terminal sequencing; Neuropeptide; Transformed insect cells

Indexed keywords

EID: 0040754576     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/arch.12     Document Type: Article

References (16)

1. Ailor E, Betenbaugh MJ. 1999. Modifying secretion and post-translational processing in insect cells. Curr Opin Biotechnol 10:142-145.
2. Audsley N, McIntosh C, Phillips JE. 1992a. Isolation of a neuropeptide from locust corpus cardiacum which influences ileal transport. J Exp Biol 173:261-274.
3. Audsley N, McIntosh C, Phillips JE. 1992b. Actions of ion-transport peptide from locust corpus cardiacum on several hindgut processes. J Exp Biol 173:275-288.
4. Hanrahan JW, Meredith J, Phillips JE, Brandys D. 1984. Methods for the study of transport and control in insect hindgut. In: Bradley TJ, Millar TA, editors. Measurement of ion transport and metabolic rate in insects. New York: Springer. p 19-68.
5. Hegedus D, Pfeifer TA, Hendry J, Theilmann DA, Grigliatti TA. 1998. A series of broad host range shuttle vectors for constitutive and inducible expression and secretion of heterologous proteins in insect cell lines. Gene 207:241-249.
6. Irvine BN, Audsley N, Lechleitner R, Meredith J, Thomson B, Phillips JE. 1988. Transport properties of locust ileum in vitro: effects of cyclic AMP. J Exp Biol 137: 361-385.
7. King DS, Meredith J, Wang Y-J, Phillips JE. 1999. Biological actions of synthetic locust ion transport peptide. Insect Biochem Mol Biol 29:11-18.
8. Liao UIS, Liu X, Harrison JF. 1996. Renal regulation of acid-base status in the American locust, Schistocerca americana. Am Zool 36:15A.
9. Macins A, Meredith J, Zhao Y, Brock HW, Phillips JE. 1999. Occurrence of ion transport peptide (ITP) and ion transport-like peptide (ITP-L) in orthopteroids. Arch Insect Biochem Physiol 40:107-118.
10. Meredith J, Ring M, Macins A, Marschall J, Cheng NN, Theilmann D, Brock HW, Phillips JE. 1996. Locust ion transport peptide (ITP): primary structure, cDNA and expression in a baculovirus system. J Exp Biol 199: 1053-1061.
11. Pfeifer TA, Hegedus DD, Grigliatti TA, Theilmann DA. 1997. Baculovirus immediate-early promotor-mediated expression of the ZeocinTM resistance gene for use as a dominant selectable marker in dipteran and lepidopteran insect cell lines. Gene 188:183-190.
12. Phillips JE, Thomson RB, Peach JL, Audsley N, Stagg AP. 1994. Mechanisms of acid-base transport and control in locust excretory system. Physiol Zool 67:95-119.
13. Phillips JE, Wiens C, Audsley N, Jeffs L, Bilgen T, Meredith J. 1996. Nature and control of chloride transport in insect absorptive epithelia. J Exp Zool 275:292-299.
14. Ring M, Meredith J, Wiens C, Macins A, Brock HW, Phillips JE, Theilmann DA. 1998. Expression of Schistocerca gregaria ion transport peptide (ITP) and its homologue (ITP-L) in a baculovirus/insect cell system. Insect Biochem Mol Biol 28:51-58.
15. Schägger H, von Jagow G. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368-379.
16. Summers MD, Smith GE. 1987. A manual of methods for baculovirus vectors and insect cell culture procedures. Tex Agric Exp Stn (Bull) 1555:1-56.