Semiquantitative chemiluminescent detection of UV-B-induced point mutations in the p53 tumor-suppressor gene

BioTechniques

Volumn 26, Issue 3, 1999, Pages 528-530

  Siefken, Wilfried ^a   Hoppe, Udo ^a   Bergemann, Jörg ^a  

^a BEIERSDORF AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; PROTEIN P53;

ALLELE; ARTICLE; CELL MUTANT; CHEMOLUMINESCENCE; CORNEA CELL; GENE ISOLATION; GENE MUTATION; INTERMETHOD COMPARISON; MUTATION RATE; POINT MUTATION; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TUMOR SUPPRESSOR GENE; ULTRAVIOLET RADIATION;

EID: 0040314949     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99263cr01     Document Type: Article

References (9)

1. Boukamp, P., R.T. Petrussevska, D. Breitkreutz, J. Hornung, A. Markham and N. Fusenig. 1988. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106:761-771.
2. Brash, D.E. 1988. UV mutagenic photoproducts in Escherichia coli and human cells: a molecular genetics perspective on human skin cancer. Photochem. Photobiol. 45:59-66.
3. Brash, D.E. 1997. Sunlight and the onset of skin cancer. Trends Genet. 13:410-414.
4. Hollstein, M., D. Sidransky, B. Vogelstein and C.C. Harris. 1991. Mutations in human cancers. Science 253:49-53.
5. Huang, M.M., N. Arnheim and M.F. Goodman. 1992. Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucleic Acids Res. 20:4567-4573.
6. Lehman, T.A., R. Modali, P. Boukamp, J. Stanek, W.P. Bennet, J.A. Welsh, R.A. Metcalf, M.R. Stampfer et al. 1993. p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 14:833-839.
7. Nakazawa, H., D. English, P.L. Randell, K. Nakazawa, N. Martel, B.K. Armstrong and H. Yamasaki. 1994. UV and skin cancer: specific p53 gene mutation in normal skin as a biologically relevant exposure measurement. Proc. Natl. Acad. Sci. USA 91:360-364.
8. Rhodes, H.C., C. Honsinger, D.M. Porter and G.D. Sorenson. 1997. Analysis of the allele-specific PCR method for the detection of neoplastic disease. Mol. Pathol. 6:49-57.
9. Soussi, T., Y. Legros, R. Lubin, K. Ory and B. Schlichtholz. 1994. Multifactoral analysis of p53 alterations in human cancer: a review. Int. J. Cancer 57:1-9.