A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA

Nature

Volumn 388, Issue 6640, 1997, Pages 397-400

  Tronchère, Hélène ^a   Wang, Jiwu ^a   Fu, Xiang Dong ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; MESSENGER RNA PRECURSOR; PROTEIN SERINE KINASE; PYRIMIDINE; SERINE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; ENZYME PURIFICATION; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION; RNA SPLICING; SPLICEOSOME;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BLOTTING, WESTERN; CELL NUCLEUS; CLONING, MOLECULAR; GENETIC COMPLEMENTATION TEST; HELA CELLS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN BINDING; RECOMBINANT PROTEINS; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICING; RNA-BINDING PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0039769985     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/41137     Document Type: Article

References (23)

1. Ruskin, B., Zamore, P. D. & Green, M. R. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 52, 207-219 (1988).
2. Krämer, A. The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem. 65, 367-490 (1996).
3. Wu, J. & Maniatis, T. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell 75, 1061-1070 (1993).
4. Kohtz, J. D. et al. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. Nature 368, 119-124 (1994).
5. 35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes Dev. 10, 1356-1368 (1996).
6. Roscigno, R. F. & Garcia-Blanco, M. A. SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome. RNA 1, 692-706 (1995).
7. Fetzer, S., Lauber, J., Will, C. L. & Lührmann, R. The [U4/6.U5] tri-snRNP-specific 27K protein is a novel SR protein which can be phosphorylated by the snRNP-associated protein kinase. RNA 3, 344-355 (1997).
8. S. Gui, J.-F., Lane, W. S. & Fu, X.-D. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369, 678-682 (1994).
9. Gui, J.-F., Tronchère, H., Chandler, S. D. & Fu, X.D. Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors. Proc. Natl Acad. Sci. USA 91, 10824-10828 (1994).
10. Colwill, K. et al. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/ arginine-rich splicing factors. J. Biol. Chem. 271, 24569-24575 (1996).
11. Fu, X.-D. The superfamily of arginine/serine-rich splicing factors. RNA 1, 663-680 (1995).
12. Manley, J. L. & Tacke, R. SR proteins and splicing control. Genes Dev. 10, 1569-1579 (1996).
13. Kitagawa, K. et al. Isolation and mapping of human homologues of an imprinted mouse gene U2af1-rs1. Genomics 30, 257-263 (1995).
14. Nabetani, A., Hatada, I., Morisaki, H., Oshimura, M. & Mukai, T. Mouse U2af1-rs1 is a neomorphic imprinted gene. Mol. Cell. Biol. 17, 789-798 (1997).
15. Hyashizaki, Y. et al. Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method. Nature Genet. 6, 33-40 (1994).
16. Yamaoka, T., Hatada, I., Kitagawa, K., Wang, X. & Mukai, T. Cloning and mapping of the U2af1-rs2 gene with a high transmission distortion in interspecific backcross progeny. Genomics 27, 337-340 (1995).
17. Zamore, P. D. & Green, M. R. Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with novel intranuclear distribution. EMBO J. 10, 207-214 (1991).
18. Rudner, D. Z., Kanaar, R., Breger, K. S. & Rio, D. C. Mutations in the small suhunit of the Drosphila U2AF splicing factor cause lethality and developmental defects. Proc. Natl Acad. Sci. USA 93, 10333-10337 (1996).
19. Zamore, P. D. & Green, M. R. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc. Natl Acad. Sci. USA 86, 9243-9247 (1989).
20. Zhang, M., Zamore, P. D., Carmo-Fonseca, M., Lamond, A. I. & Green, M. R. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc. Natl Acad. Sci. USA 89, 8769-8773 (1992).
21. Hannon, G. J., Dmetrick, D. & Beach, D. Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins. Genes Dev. 7, 2378-2391 (1993).
22. Harlow, E. & Lane, D. Antibodies: A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1988).
23. Zervos, A. S., Gyuris, J. & Brent, R. Mxi, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell 72, 223-232 (1993).