The γ subunit is a specific component of the Na,K-ATPase and modulates its transport function

EMBO Journal

Volumn 16, Issue 14, 1997, Pages 4250-4260

  Béguin, Pascal ^a   Wang, Xinyu ^a   Firsov, Dimitri ^a   Puoti, Alessandro ^a   Claeys, Dirk ^a   Horisberger, Jean Daniel ^a   Geering, Käthi ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Modulation of Na,K pump; Na,K ATPase subunit; Proteolipids; Xenopus oocyte

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ISOENZYME; MEMBRANE PROTEIN; POTASSIUM ION; SODIUM ION;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ENZYME ACTIVATION; ENZYME SUBUNIT; NONHUMAN; OOCYTE; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; RADIOIMMUNOASSAY; SODIUM TRANSPORT; STRUCTURE ACTIVITY RELATION; XENOPUS;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL MEMBRANE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPLASMIC RETICULUM; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; NA(+)-K(+)-EXCHANGING ATPASE; OOCYTES; PATCH-CLAMP TECHNIQUES; PEPTIDE BIOSYNTHESIS; PEPTIDES; POTASSIUM; PRECIPITIN TESTS; PROTEIN BINDING; RATS; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; XENOPUS;

ANIMALIA;

EID: 0039438642     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.14.4250     Document Type: Article

References (36)

1. Ackermann, U. and Geering, K. (1990) Mutual dependence of Na,K-ATPase α-subunits and β-subunits for correct posttranslational processing and intracellular transport. FEBS Lett., 269, 105-108.
2. + channel activity in Xenopus oocytes. Proc. Natl Acad. Sci. USA, 92, 6092-6096.
3. Beggah, A.T., Jaunin, P. and Geering, K. (1997) Role of glycosylation and disullide bond formation in the β subunit of Na,K-ATPase. J. Biol. Chem., 272, 10318-10326.
4. Béguin, P., Peitsch, M.C. and Geering, K. (1996) α-1 but not α-2 or α-3 isoforms of Na,K-ATPase are efficiently phosphorylated in a novel protein kinase C motif. Biochemistry, 35, 14098-14108.
5. Claeys, D., Karapetian, O., Saraga, E., Schreyer, M., Louis, J., Acha-Orbea, H., Blum, A.L. and Kraehenbuhl, J.P. (1994) Mouse mammary tumor virus superantigens and murine autoimmune gastritis. Gastroenterology, 107, 924-933.
6. +)-ATPase proteolipid labeled with a photoaffinity derivative of ouabain. Biochim. Biophys. Acta. 686, 7-12.
7. Forbush, B., III, Kaplan, J.H. and Hoffman, J.F. (1978) Characterization of a new photoaffinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the Na,K-ATPase. Biochemistry, 17, 3667-3676.
8. Fujii, J., Ueno, A., Kitano, K., Tanaka, S., Kadoma, M. and Tada, M. (1987) Complete complementary DNA-derived amino acid sequence of canine cardiac phospholamban. J. Clin. Invest., 79, 301-304.
9. 2 terminus of the β subunit with the α subunit. J. Cell Biol., 133, 1193-1204.
10. +)-ATPase subunits. Biochem. J., 20, 6684-6691.
11. Hardwicke, P.M. and Freytag, J.W. (1981) A proteolipid associated with Na,K-ATPase is not essential for ATPase activity. Biochem. Biophys. Res. Commun., 102, 250-257.
12. Horisberger, J.-D. (1994) The Na,K-ATPase: Structure-Function Relationship. R.G.,Landes Company, Austin, TX.
13. Jaisser, F., Canessa, C.M, Horisberger, J.D. and Rossier, B.C. (1992) Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase: the β-subunit modulates potassium activation of the Na,K-pump. J. Biol. Chem., 267, 16895-16903.
14. Jaisser, F., Jaunin, P., Geering, K., Rossier, B.C. and Horisberger, J.D. (1994) Modulation of the Na,K-pump function by βsubunit isoforms. J. Gen. Physiol., 103, 605-623.
15. Jaunin, P., Horisberger, J.D., Richter, K., Good, P.J., Rossier, B.C. and Geering, K. (1992) Processing, intracellular transport, and functional expression of endogenous and exogenous α-β3 Na,K-ATPase complexes in Xenopus oocytes. J. Biol. Chem., 267, 577-585.
16. Kolodziej, P.A. and Young, R.A. (1991) Epitope tagging and protein surveillance. Methods Enzymol., 194, 508-519.
17. Kozak, M. (1987) An analysis of 5′ non-coding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res., 15, 8125-8148.
18. Levenson, R. (1994) Isoforms of the Na,K-ATPase: family members in search of function. Rev. Physiol. Biochem. Pharmacol., 123, 1-45.
19. 125I]iodoazidocymarin. J. Biol. Chem., 259, 10533-10538.
20. Lutsenko, S. and Kaplan, J.H. (1993) An essential role for the extracellular domain of the Na,K-ATPase β-subunit in cation occlusion. Biochemistry, 32, 6737-6743.
21. +-ATPase. Am. J. Physiol., 37, C1207-C1214.
22. Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. and Green, M.R. (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res., 12, 7035-7056.
23. Mercer, R.W., Biemesderfer, D., Bliss, D.P., Collins, J.H. and Forbush, B. (1993) Molecular cloning and immunological characterization of the γ-polypeptide, a small protein associated with the Na,K-ATPase. J. Cell Biol., 121, 579-586.
24. Moorman, J.R., Palmer, C.J., John, J.E., III, Durieux, M.E. and Jones, L.R. (1992) Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes. J. Biol. Chem., 267, 14551-14554.
25. Morrison, B.W., Moorman, J.R., Kowdley, G.C., Kobayashi, Y.M., Jones, L.R. and Leder, P. (1995) Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes. J. Biol. Chem., 270, 2176-2182.
26. +-ATPase of Saccharomyces cerevisiae. J. Biol. Chem., 267, 6425-6428.
27. Puoti, A., May, A., Canessa, C.M., Horisberger, J.D., Schild, L. and Rossier, B.C. (1995) The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells. Am. J. Physiol., 38, C188-C197.
28. +. J. Membr. Biol., 121, 177-187.
29. Reeves, A.S., Collins, J.H. and Schwartz, A. (1980) Isolation and characterization of (Na,K)-ATPase proteolipid. Biochem. Biophys. Res. Commun., 95, 1591-1598.
30. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
31. Scheiner-Bobis, G. and Farley, R.A. (1994) Subunit requirements for expression of functional sodium pumps in yeast cells. Biochim. Biophys. Acta. 1193, 226-234.
32. + ions. J. Biol. Chem., 271, 10309-10316.
33. Smith, D.B. and Johnson, K.S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene, 67, 31-40.
34. +-ATPase and its localization in A6 kidney cells. Am. J. Physiol., 256, F1034-F1043.
35. von Heijne, G. and Gavel, Y. (1988) Topogenic signals in integral membrane proteins. Eur. J. Biochem., 174, 671-678.
36. + pump of Bufo. J. Physiol., 491, 579-594.