Cell-associated, non-replicating strand(+) hepatitis C virus-RNA shedding in cervicovaginal secretions from chronically HCV-infected women

Journal of Clinical Virology

Volumn 27, Issue 3, 2003, Pages 247-251

  Bélec, Laurent ^a,b   Legoff, Jérôme ^b   Si Mohamed, Ali ^b   Bloch, Francis ^c   Matta, Mathieu ^b   Mbopi Keou, François Xavier ^d   Payan, Christopher ^e  

^a INSERM   (France)

^b Laboratoire de Virologie   (France)

^c Assistance publique hôpitaux de Paris   (France)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

^e ANGERS UNIVERSITY HOSPITAL   (France)

Author keywords

Female genital tract; HCV

Indexed keywords

BUFFER; HEMOGLOBIN; MARKER; VIRUS RNA;

ADULT; ARTICLE; CELL; CHRONIC DISEASE; CHRONIC HEPATITIS; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; HEPATITIS C; HEPATITIS C VIRUS; HOSPITALIZATION; HUMAN; PATIENT SELECTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUALITATIVE ANALYSIS; SPERM; VAGINA SECRETION; VIRUS DETECTION; VIRUS EXCRETION; VIRUS REPLICATION; VIRUS SHEDDING;

ADOLESCENT; ADULT; CERVIX UTERI; FEMALE; HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMANS; MIDDLE AGED; POLYMERASE CHAIN REACTION; RNA, VIRAL; VAGINA; VIRUS REPLICATION; VIRUS SHEDDING;

EID: 0038826658     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1386-6532(02)00178-6     Document Type: Article

References (27)

1. Ackerman Z., Paltiel O., Glikberg F., Ackerman E. Hepatitis C virus in various human body fluids: a systematic review. Hepatol. Res. 11:1998;26-40.
2. Bélec L., Meillet D., Lévy M., Georges A., Tévi-Bénissan C., Pillot J. Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays. Clin. Diagn. Lab. Immunol. 2:1995;57-61.
3. Bélec L., Authier J., Chazaud B., Piedouillet C., Balovatz-Meinon G., Gherardi K. Interleukin (IL)-1β and IL-1β mRNA production in normal and diseased skeletal muscle assessed by immunocytochemistry, immunoblotting and reverse transcriptase-nested polymerase chain reaction. J. Exp. Appl. Neurobiol. 56:1997;651-663.
4. Boom R., Sol J., Salimans M., Lansen C., Wertheim Van Dillen P., Van des Noordaa. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:1990;495-503.
5. Cadwell S.H., Sue M., Bowden J.H., Dickson R.C., Driscoll C.J., Yeaton P., Stevenson W.C., Ishitani M.B., McCullough C.S., Pruett T.L., Lovell M.A. Hepatitis C virus in body fluids after liver transplantation. Liver Transpl. Surg. 2:1996;124-129.
6. Chen M., Yun Z.-B., Sällberg M., Schvarcz, Bergquist I., Berglund H.-B., Sönnerborg A. Detection of hepatitis C virus RNA in the cell fraction of saliva before and after oral surgery. J. Med. Virol. 43:1995;223-226.
7. Chomont N., Gresenguet G., Levy M., Hocini H., Becquart P., Matta M., Tranchot-Diallo J., Andreoletti L., Carreno M.P., Kazatchkine M.D., Bélec L. Detection of Y chromosome DNA as evidence of semen in cervicovaginal secretions of sexually active women. Clin. Diagn. Lab. Immunol. 8:2001;955-958.
8. Fabris P., Infantolino D., Biasin M.R., Marchelle G., Venza E., Terribile Wiel Marin V., Benedetti P., Tositti G., Manfrin V., de Lalla F. High prevalence of HCV-RNA in the saliva cell fraction of patients with chronic hepatitis C but no evidence of HCV transmission among sexual partners. Infection. 2:1999;86-91.
9. Gameiro R., Venneno T., Prieto E., Castro R., Carnall V., Canas-Ferreira W., Exposto F. Vaginal shedding of hepatitis C virus. Int. J. STD AIDS. 12:2001;717-721.
10. Hsu H.H., Wright T.L., Luba D., Martin M., Feinstone S.M., Garcia G., Greenberg H.B. Failure to detect hepatitis C virus genome in human secretions with the polymerase chain reaction. Hepatology. 14:1991;763-767.
11. Imhof B.A., Dunon D. Leukocyte migration and adhesion. Adv. Immunol. 58:1995;345-416.
12. Johansson E.L., Rudin A., Wassen L., Holmgren J. Distribution of lymphocytes and adhesion molecules in human cervix and vagina. Immunology. 96:1999;272-277.
13. Kurauchi O., Furui T., Itakura A., Ishiko H., Sugiyama M., Ohno Y., Ando H., Tanamura A., Ishida T., Nawa A., Nomura S., Mizutani S., Tomoda Y. Studies on transmission of hepatitis C virus from mother to child in the perinatal period. Arch. Gynecol. Obstet. 253:1993;121-126.
14. Lanford R.E., Sureau C., Jacob J.R., White R., Fuerst T. Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT-PCR. Virology. 202:1994;606-614.
15. Leruez-Ville M., Kunstmann J.-M., de Almeida M., Rouzioux C., Chaix M.-L. Detection of hepatitis C virus in the semen of infected men. Lancet. 356:2000;42.
16. Liou T.-C., Chang T.-T., Young K.-C., Lin X.-Z., Wu H.-L. Detection of HCV RNA in saliva, urine, seminal fluids and ascites. J. Med. Virol. 37:1992;197-202.
17. Muller H., Pfaff E., Goeser T., Kallinowski B., Solbach C., Theillmann L. Peripheral blood leukocytes serve as a possible extrahepatic site for hepatitis C virus replication. J. Gen. Virol. 74:1993;669-676.
18. Payan C., Bloch F., Gaultier I., Bélec L. Single-step reverse transcription-polymerase chain reaction for hepatitis C virus RNA with DNA enzyme immunoassay hybridization. J. Virol. Meth. 53:1995;167-175.
19. Payan C., Dupré T., Bélec L. Detection of hepatitis C virus RNA by a reliable, optimized single-step reverse transcription polymerase chain reaction. Res. Virol. 146:1995;363-370.
20. Payan C., Véal N., Crescenzo-Chaigne B., Bélec L., Pillot J. New quantitative assay of hepatitis B and C viruses by competitive PCR using alternative internal sequences. J. Virol. Meth. 65:1997;299-305.
21. Quian C., Camps J., Maluenda M., Cieveira M., Prieto J. Replication of hepatitis C in peripheral blood mononuclear cells: effect of alpha-interferon therapy. J. Hepatol. 16:1992;380-383.
22. Saarinen J., Saarelainen R., Lindros K.O. A rapid method to study heterogeneous gene expression in liver direct assay of messenger RNA from periportal and perivenous cell lysates. Hepatology. 17:1993;466-469.
23. Sanderink G.J., Van Rijn H.J. Quantitative measurement of plasma hemoglobin by second derivative spectrophotometry. Clin. Chim. Acta. 28:1985;65-73.
24. Tang Z., Yang D., Hao L., Tang Z., Huang Y., Wang S. Detection and significance of HCV RNA in saliva and seminal fluid and vaginal discharge in patients with hepatitis C. J. Tongji Med. Univ. 16:1996;11-13.
25. Wejstal R. Sexual transmission of hepatitis C virus. J. Hepatol. 13:1999;S92-S95.
26. Young K.-C., Chuang T.-T., Liou T.-C., Wu H.-L. Detection of hepatitis C virus RNA in peripheral blood mononuclear cells in saliva. J. Med. Virol. 41:1993;55-60.
27. Zanetti A.R., Tanzi E., Paccagnini S., Principi N., Pizzocolo G., Caccamo M.L., D'Amico E., Gambie G., Vecchi L. Mother-to-child transmission of hepatitis C virus. Lancet. 345:1995;289-291.