Collagen expression in tissue engineered cartillage of aged human articular chondrocytes in a rotating bioreactor

International Journal of Artificial Organs

Volumn 26, Issue 4, 2003, Pages 319-330

  Marlovits, Stefan ^a,b   Tichy, B ^a   Truppe, M ^b   Gruber, D ^a   Schlegel, W ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b LUDWIG BOLTZMANN INSTITUTE FOR LUNG VASCULAR RESEARCH   (Austria)

Author keywords

Aging; Bioreactor; Cartilage; Chondrocytes; Tissue engineering

Indexed keywords

BIOCONVERSION; BIOREACTORS; BODY FLUIDS; CELL CULTURE; COLLAGEN; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; MOLECULAR BIOLOGY; MONOLAYERS; SCAFFOLDS (BIOLOGY); SHEAR STRESS;

BIOREACTOR; CHONDROCYTES; COLLAGEN EXPRESSION; HUMAN ARTICULAR CHONDROCYTES; IMMUNOHISTOCHEMISTRY; MONOLAYER CULTURE; ROTATING WALL VESSELS; SINGLE-CELL SUSPENSIONS; THREE-DIMENSIONAL ASSEMBLY; TISSUES ENGINEERINGS;

CARTILAGE;

COLLAGEN; COLLAGEN TYPE 1; COLLAGEN TYPE 2;

AGED; ARTICLE; BIOREACTOR; BLOOD VESSEL WALL; CARTILAGE CELL; CARTILAGE MATRIX; CARTILAGINOUS TISSUE; CELL AGGREGATION; CELL AGING; CELL DIFFERENTIATION; CELL STRUCTURE; CELL SUSPENSION; CELL TRANSFORMATION; CLINICAL ARTICLE; ELECTRON MICROSCOPY; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MOLECULAR BIOLOGY; MONOLAYER CULTURE; OXYGENATION; PROTEIN EXPRESSION; RANDOMIZATION; ROTATION; SHEAR STRESS; TISSUE ENGINEERING; TRANSMISSION ELECTRON MICROSCOPY;

EID: 0038784390     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139880302600407     Document Type: Article

References (35)

1. Hunziker EB. Articular cartilage repair: Basic science and clinical progress. A review of the current status and prospects. Osteoarthr Cartil 2002; 10: 432-63.
2. Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 1994; 331: 889-95.
3. Heinegard D, Oldberg A. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J 1989; 3: 2042-51.
4. Spaulding GF, Jessup JM, Goodwin TJ. Advances in cellular construction. J Cell Biochem 1993; 51: 249-51.
5. Schwarz RP, Goodwin TJ, Wolf DA. Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity. J Tissue Cult Methods 1992; 14: 51-8.
6. Schnabel M, Marlovits S, Eckhoff G, Fichtel I, Gotzen L, Vecsei V, Schlegel J. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthr Cartil 2002; 10: 62-70.
7. Prewett TL, Goodwin TJ, Spaulding GF. Three-dimensional modeling of T-24 human bladder carcinoma cell line: A new simulated microgravity culture vessel. J Tissue Cult Methods 1993; 15: 29-36.
8. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique. Third Edition ed. New York: Wiley-Liss, 1994.
9. Bozzola JJ, Russel LD. Electron Microscopy: Principles and Techniques for Biologists. Boston: Jones and Bartlett Publishers, 1992.
10. Benya PD, Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 1982; 30: 215-24.
11. Bassleer C, Gysen P, Foidart JM, Bassleer R, Franchimont P. Human chondrocytes in tridimensional culture. In Vitro Cell Dev Biol 1986; 22: 113-9.
12. Sittinger M, Schultz O, Keyszer G, Minuth WW, Burmester GR. Artificial tissues in perfusion culture. Int J Artif Organs 1997; 20: 57-62.
13. Koch RJ, Gorti GK. Tissue engineering with chondrocytes. Facial Plast Surg 2002; 18: 59-68.
14. Pazzano D, Mercier KA, Moran JM, Fong SS, Di Biasio DD, Rulfs JX, Kohles SS, Bonassar LJ. Comparison of chondrogenesis in static and perfused bioreactor culture. Biotechnol Prog 2000; 16: 893-6.
15. Hall BK, Van Exan RJ. Induction of bone by epithelial cell products. J Embryol Exp Morphol 1982; 69: 37-46.
16. Dean RC, Jr., Karkare SB, Ray NG, Runstadler PW, Jr., Venkatasubramanian K. Large-scale culture of hybridoma and mammalian cells in fluidized bed bioreactors. Ann N Y Acad Sci 1987; 506: 129-46.
17. Lee DW, Piret JM, Gregory D, Haddow DJ, Kilburn DG. Polystyrene macroporous bead support for mammalian cell culture. Ann N Y Acad Sci 1992; 665: 137-45.
18. Cherry RS, Hulle CT. Cell death in the thin films of bursting bubbles. Biotechnol Prog 1992; 8: 11-8.
19. Prokop A, Rosenberg MZ. Bioreactor for mammalian cell culture. Adv Biochem Eng Biotechnol 1989; 39: 29-71.
20. Minuth WW, Sittinger M, Kloth S. Tissue engineering: Generation of differentiated artificial tissues for biomedical applications. Cell Tissue Res 1998; 291: 1-11.
21. Wu F, Dunkelman N, Peterson A, Davisson T, De La Torre R, Jain D. Bioreactor development for tissue-engineered cartilage. Ann N Y Acad Sci 1999; 875: 405-411.
22. Duray PH, Hatfill SJ, Pellis NR. Tissue culture in microgravity. Sci Med (Phila) 1997; 4: 46-55.
23. Tsao YD, Goodwin TJ, Wolf DA, Spaulding GF. Responses of gravity level variations on the NASA/JSC bioreactor system. Physiologist 1992; 35 (suppl 1): S49-50.
24. Jessup JM, Goodwin TJ, Spaulding G. Prospects for use of microgravity-based bioreactors to study three-dimensional host-tumor interactions in human neoplasia. J Cell Biochem 1993; 51: 290-300.
25. Hughes-Fulford M, Lewis ML. Effects of microgravity on osteoblast growth activation. Exp Cell Res 1996; 224: 103-109.
26. Goodwin TJ, Jessup JM, Wolf DA. Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels. In Vitro Cell Dev Biol 1992; 28A: 47-60.
27. Becker JL, Prewett TL, Spaulding GF, Goodwin TJ. Three-dimensional growth and differentiation of ovarian tumor cell line in high aspect rotating-wall vessel: Morphologic and embryologic considerations. J Cell Biochem 1993; 51: 283-9.
28. Tschopp A, Cogoli A, Lewis ML. Bioprocessing in space: Human cells attach to beads in microgravity. J Biotechnol 1984; 1: 287-94.
29. Lelkes PI, Galvan DL, Hayman GT, Goodwin TJ, Chatman DY, Cherian S, Garcia RM, Unsworth BR. Simulated microgravity conditions enhance differentiation of cultured PC12 cells towards the neuroendocrine phenotype. In Vitro Cell Dev Biol Anim 1998; 34: 316-25.
30. Vunjak-Novakovic G, Martin I, Obradovic B, Treppo S, Grodzinsky AJ, Langer R, Freed LE. Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. J Orthop Res 1999; 17: 130-8.
31. Goldring MB. Human chondrocyte cultures as models of cartilage-specific gene regulation. In: Jones GE, ed. Human Cell Culture Protocols. Totowa, New Jersey: Humana Press, 1996; 217-232.
32. Green WT, Jr. Behavior of articular chondrocytes in cell culture. Clin Orthop 1971; 75: 248-60.
33. Shepard N, Mitchell N. The localization of proteoglycan by light and electron microscopy using safranin O. A study of epiphyseal cartilage. J Ultrastruct Res 1976; 54: 451-60.
34. Christensen SB, Reimann I. Differential histochemical staining of glycosaminoglycans in the matrix of osteoarthritic cartilage. Acta Pathol Microbiol Scand [A] 1980; 88: 61-8.
35. Poole CA, Glant TT, Schofield JR. Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons. J Histochem Cytochem 1991; 39: 1175-87.