Characterization of a polysaccharide-protein complex from Ganoderma tsugae mycelium by size-exclusion chromatography combined with laser light scattering

Journal of Biochemical and Biophysical Methods

Volumn 56, Issue 1-3, 2003, Pages 243-252

  Peng, Yanfei ^a   Zhang, Lina ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Conformation; Ganoderma tsugae; Light scattering; Molecular mass; Polysaccharide; Size exclusion chromatography

Indexed keywords

FUNGAL PROTEIN; POLYSACCHARIDE; PROTEIN; SODIUM CHLORIDE; TRYPSIN;

AQUEOUS SOLUTION; ARTICLE; CONFORMATION; FRACTIONATION; GANODERMA; GEL PERMEATION CHROMATOGRAPHY; LASER; LIGHT SCATTERING; MOLECULAR WEIGHT; MYCELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; REFRACTION INDEX; SOLUBILITY; ULTRAVIOLET RADIATION;

GANODERMA TSUGAE;

EID: 0038771206     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(03)00062-9     Document Type: Article

References (21)

1. Zhang L., Xu X., Jiang G., Iijma H., Tsuchiya H. Aggregation of Aeromonas gum in aqueous solution. Polymer Journal. 31:1999;150-153.
2. Zhang L., Xu X., Pan S. Molecular size and aggregation behavior of Erwinia gum in aqueous solution. Journal of Polymer Science, Part B. 38:2000;1352-1358.
3. Zhang L., Zhang X., Zhou Q., Zhang P., Zhang M., Li X. Triple helix of β-D-glucan from Lentinus edodes in 0.5M NaCl aqueous solution characterized by light scattering. Polymer Journal. 33:2001;317-321.
4. Tsukagoshi S., Hashimoto Y., Fujii G., Kobayashi H., Nomoto K., Orita K. Krestin (PSK). Cancer Treatment Reviews. 11:1984;131-155.
5. Matsunaga K., Hosokawa A., Oohara M., Sugita N., Harada M., Nomoto K. Direct action of a protein-bound polysaccharide, PSK, on transforming growth factor-β Immunopharmacology. 40:1998;219-230.
6. Zhang J., Wang G., Li H., Zhuang C., Mizuno T., Ito H.et al. Anti-tumor active protein-containing glycans from the Chinese mushroom Songshan lingzhi, Ganoderma tsugae mycelium. Bioscience, Biotechnology, and Biochemistry. 58:1994;1202-1205.
7. Mizuno T., Saito H., Nishitoba T., Kawagishi H. Antitumor-active substances from mushrooms. Food Reviews International. 11:1995;23-61.
8. Skvortsov I.M., Ignatov V.V. Extracellular polysaccharides and polysaccharide-containing biopolymers from Azospirillum species: properties and the possible role in interaction with plant roots. FEMS Microbiology Letters. 165:1998;223-229.
9. Liu F., Ooi I.V.E.C. Immunomodulation and anti-cancer activity of polysaccharide-protein complex. Current Medicinal Chemistry. 6:1999;1-15.
10. Chen J., Zhang L., Yu D., Zhu R. Chemical components and solution properties of a glycan-protein complex from Ganoderma lucidum mycelium. Chemical Journal of Chinese Universities. 21:2000;961-964.
11. Connolly S., Fenyo J.C., Vandevelde M.C. Heterogeneity and homogeneity of an arabinogalactan-protein: Acacia senegal gum. Food Hydrocolloids. 1:1987;477-480.
12. Connolly S., Fenyo J.C., Vandevelde M.C. Effect of a proteinase on the macromolecular distribution of Acacia senegal gum. Carbohydrate Polymers. 8:1988;23-32.
13. Qi W., Fong C., Lamport D.T.A. Gum-arabic glycoprotein is a twisted hairy rope. A new model based on O-galactosylhydroxyproline as the polysaccharide attachment site. Plant Physiology. 96:1991;848-855.
14. Lucey J.A., Srinivasan M., Singh H., Munro P.A. Characterization of commercial and experimental sodium caseinates by mutiangle laser light scattering and size-exclusion chromatography. Journal of Agricultural and Food Chemisty. 48:2000;1610-1616.
15. Picton L., Bataille G., Muller G. Analysis of a complex polysaccharide (gum Arabic) by muti-angle laser light scattering coupled on-line to size exclusion chromatography and flow field flow fractionation. Carbohydrate Polymers. 42:2000;23-31.
16. Eo S.K., Kim Y.S., Lee C.K., Han S.S. Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. Journal of Ethnopharmacology. 72:2000;475-781.
17. Peng Y., Zhang L., Xu X., Chen L. Chemical components and molecular weight of water-soluble polysaccharides-protein complex from Ganoderma tsugae mycelia. Chinese Journal of Polymer Science. 21:2003;303-310.
18. Staub A.M. Removal of protein - Sevag method. Methods in Carbohydrate Chemistry. 5:1965;5-6.
19. Majdoub H., Roudesli S., Picton L., Cerf D.L., Muller G., Grisel M. Prickly pear nopals pectin from Opuntia ficus-indica physico-chemical study in dilute and semi-dilute solutions. Carbohydrate Polymers. 46:2001;69-79.
20. Ghosh S., Reed W.F. New characteristic signatures from time-dependent static light scattering during polymer depolymerization, with application to proteoglycan subunit degradation. Biopolymers. 35:1995;435-450.
21. Zhang L., Qiu X., Xu J., Du Y., Qian B. Studies on fractionation and molecular weights of Chinese lacquer polysaccharide. Carbohydrate Polymers. 19:1992;161-166.