Amplification of T cells from human cord blood in serum-deprived culture stimulated with stem cell factor, interleukin-7 and interleukin-2

Bone Marrow Transplantation

Volumn 31, Issue 8, 2003, Pages 713-723

  Sanchez, M ^a   Alfani, E ^a   Migliaccio, A R ^a,b   Bonfini, T ^c   Migliaccio, G ^a,b  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b UNIVERSITY OF CHIETI   (Italy)

^c SPIRITO SANTO HOSPITAL   (Italy)

Author keywords

Cord blood; Ex vivo amplification; Interleukin 2; Interleukin 7; Serum free cultures; Stem cell factor; T lymphocytes

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; CYTOKINE; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 7; STEM CELL FACTOR; T LYMPHOCYTE RECEPTOR BETA CHAIN;

ADOPTIVE IMMUNOTHERAPY; ARTICLE; CELL NUCLEUS; CELL SPECIFICITY; CONTROLLED STUDY; GENE REARRANGEMENT; HUMAN; HUMAN CELL; IMMUNOMODULATION; LYMPHOCYTE ACTIVATION; LYMPHOCYTE COUNT; LYMPHOCYTE CULTURE; MONONUCLEAR CELL; NEUTROPHIL; PRIORITY JOURNAL; PROMYELOCYTE; PROTEIN EXPRESSION; SINGLE STRAND CONFORMATION POLYMORPHISM; T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; TRANSPLANTATION; UMBILICAL CORD BLOOD;

CD8-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL DIVISION; CULTURE MEDIA, SERUM-FREE; DELIVERY, OBSTETRIC; FETAL BLOOD; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL MOBILIZATION; HUMANS; IMMUNOPHENOTYPING; INFANT, NEWBORN; INTERLEUKIN-2; INTERLEUKIN-7; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELL FACTOR; STEM CELLS; T-LYMPHOCYTES;

EID: 0038702467     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bmt.1703904     Document Type: Article

References (46)

1. Gluckman E. Current status of umbilical cord blood hematopoietic stem cell transplantation. Exp Hematol 2000; 28: 1197-1205.
2. Rubinstein P, Carrier C, Scaradavou A et al. Outcomes among 562 recipients of placental-blood transplants from unrelated donors. N Engl J Med 1998; 339: 1565-1577.
3. Hassan J, Reen DJ. Cord blood CD4+CD45RA+ T cells achieved a lower magnitude of activation when compared with their adult counterparts. Immunology 1997; 90: 397-401.
4. Harris DT, Schumacher MJ, Locascio J et al. Phenotypic and functional immaturity of human umbilical cord blood T lymphocytes. Proc Natl Acad Sci USA 1992; 89: 10006-10010.
5. Risdon G, Gaddy J, Horie et al. Alloantigen priming induces a state of unresponsiveness in human umbilical cord blood T cells. Proc Natl Acad Sci USA 1995; 92: 2413-2417.
6. Kolb HJ, Mittermuller J, Clemm C et al. Donor leukocyte transfusion for treatment of recurrent chronic myelogeneous leukemia in marrow transplant patients. Blood 1990; 76: 2462-2465.
7. Dazzi F, Szydlo RM, Goldman JM. Donor lymphocyte infusion for relapse of chronic myeloid leukemia after allogeneic stem cell transplant: where we now stand. Exp Hematol 1999; 27: 1477-1486.
8. Gross S, Pyatt DW, Shpall EJ et al. Ex vivo expansion of CD3-cells from cord blood for use as donor lymphocyte infusions. Exp Hematol 2000; 28(Suppl. 1): 89 (Abstr. 185).
9. Carlens S, Gilljam M, Remberger M et al. Ex vivo T lymphocyte expansion for retroviral transduction: influence of serum-free media on variations in cell expansion rate and lymphocyte subset distribution. Exp Hematol 2000; 28: 1137-1146.
10. Skea D, Chang NH, Hedge R et al. Large ex vivo expansion of human umbilical cord blood CD4+ and CD8+ T cells. J Hematother 1999; 8: 129-139.
11. Azuma H, Yamada Y, Shibuya-Fujiwara N et al. Functional evaluation of ex vivo expanded cord blood lymphocytes: possible use for adoptive cellular immunotherapy. Exp Hematol 2002; 30: 346-351.
12. Bonini C, Ferrari G, Verzeletti S et al. HSV-TK gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia. Science 1997; 276: 1719-1724.
13. Peschon JJ, Morrissey PJ, Grabstein KH et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med 1994; 180: 1955-1960.
14. Hofmeister R, Khaled AR, Benbernou N et al. Interleukin-7: physiological roles and mechanisms of action. Cytokine Growth Factor Rev 1999; 10: 41-60.
15. Sadlack B, Kuhn R, Schorle H et al. Development and proliferation in lymphocytes in mice deficient for both interleukins-2 and -4. Eur J Immunol 1994; 24: 281-284.
16. Kuhn R, Rajevsky K, Muller W. Generation and analysis of interleukin-4 deficient mice. Science 1991; 254: 707-710.
17. Gluckman E, Rocha V, Boyer-Chammard A et al. Outcome of cord-blood transplantation from related and unrelated donors. N Engl J Med 1997; 337: 373-381.
18. Forte L, Migliaccio G, Sanchez M, Migliaccio AR et al. Effects of cell banking manipulations on ex vivo amplification of umbilical cord blood. Ann Inst Super Sanità 2000; 36: 333-342.
19. Sanchez M, Alfani E, Visconti G et al. Thymus-independent T-cell differentiation in vitro. Br J Haematol 1998; 103: 1198-1205.
20. Migliaccio G, Migliaccio AR, Adamson JW. In vitro differentiation of human granulocyte/macrophage and erythroid progenitors: comparative analysis of the influence of recombinant human erythropoietin, G-CSF, GM-CSF, and IL-3 in serum-supplemented and serum-deprived cultures. Blood 1988; 72: 248-256.
21. Roncarolo MG, Bigler M, Ciuti E et al. Immune responses by cord blood cells. Blood Cells 1994; 20: 573-586.
22. Choi Y, Kotzin B, Herron L et al. Interaction of Staphylococcus aureus toxin 'superantigens' with human T-cells. Proc Natl Acad Sci USA 1989; 86: 8941-8945.
23. Yamamoto K, Masuko-Hongo K, Tanaka A et al. Establishment and application of a novel T-cell clonality analysis using single-strand conformation polymorphism of T-cell receptor messenger signals. H Immunol 1996; 48: 23-31.
24. Maniatis T, Fritsch EF and Sambrook J. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Lab. Press: NY, 1989.
25. + cord blood cells in the presence of recombinant human stem cell factor. Blood 1992; 79: 2620-2627.
26. Migliaccio AR, Migliaccio G, Adamson JW. Expansion of human neonatal progenitor cells in vitro under serum-deprived conditions. Blood Cells 1994; 20: 424-428.
27. Rossmanith T, Schroder B, Bug G et al. Interleukin 3 improves the ex vivo expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of scid repopulating cells. Stem Cells 2001; 19: 313-320.
28. + cells in vitro. Nat Med 1996; 2: 46-51.
29. Plum J, De Smedt M, Defresne M-P et al. Interleukin-7 is a critical growth factor in early human T-cell development. Blood 1996; 88: 4239-4245.
30. Hirayama F, Aiba Y, Ikebuchi K et al. Differentiation in culture of murine primitive lymphohematopoietic progenitors toward T-cell lineage. Blood 1999; 93: 4187-4195.
31. Lima M, Almeida J, dos Ajos Teixeira M et al. The 'ex vivo' pattern of CD2/CD7, CD57/CD11c, CD387CD11b, CD45RA/CD45RO, and CD11a/HLA-DR expression identify acute/early and chronic/late NK-cell activation state. Blood Cells Mol Dis 2002; 28: 181-190.
32. Alfani E, Migliaccio AR, Sanchez M et al. Characterization of the T-cell receptor repertoire of neonatal T cells by RT-PCR and single strand conformation polymorphism analysis. Bone Marrow Transplant 2000; 26: 83-89.
33. Brandt J, Briddell RA, Srour EF et al. Role of c-kit ligand in the expansion of human hematopoietic progenitor cells. Blood 1992; 79: 634-641.
34. Parkin J, Cohen B. An overview of the immune system. Lancet 2001; 357: 1777-1789.
35. Von Freeden-Jeffry U, Vieira P, Lucian LA et al. Lymphopenia in interleukin (IL)-7 gene-delete mice identifies IL-7 as a non-redundant cytokine. J Exp Med 1995; 181: 1519-1526.
36. Wiles MV, Ruiz P, Imhof BA. Interleukin-7 expression during mouse thymus development. Eur J Immunol 1992; 22: 1037-1042.
37. Swain SL, Bradley LM, Croft M et al. Helper T-cell subsets: phenotype, function and the role of lymphokines in their development. Immunol Rev 1991; 123: 115-144.
38. Spellberg B, Edwards Jr JE. Type 1/type 2 immunity in infectious diseases. Clin Infect Dis 2001; 32: 76-102.
39. Chervenak R, Dempsey D, Soloff RS, Smithson G. In vitro growth of bone marrow resident T-cell precursors supported by mast-cell growth factor and IL-3. J Immunol 1992; 149: 2851-2856.
40. Yeoman H, Clark DR, De Luca D. Development of CD4 and CD8 single positive T cells in human thymus organ culture: IL-7 promotes human T-cell production by supporting immature T cells. Dev Comp Immunol 1996; 20: 241-263.
41. Tagoh H, Kishi H, Okumura A et al. Induction of recombination activating gene expression in a human lymphoid progenitor cell line: requirement of two separate signals from stromal cells and cytokines. Blood 1996; 88: 4463-4473.
42. Garcia-Ojeda ME, Dejbakhsh-Jones S, Weissman IL, Strober S. An alternate pathway for T-cell development supported by the bone marrow microenvironment: recapitulation of thymic maturation. J Exp Med 1998; 187: 1813-1823.
43. Takeshita T, Asao H, Ohtani K et al. Cloning of the gamma chain of the human IL-2 receptor. Science 1992; 257: 379-382.
44. Noguchi M, Nakamura Y, Russell SM et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. Science 1993; 262: 1877-1880.
45. Kondo M, Takeshita T, Ishii N et al. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptor for IL-2 and IL-4. Science 1993; 262: 1874-1877.
46. Russell SM, Keegan AD, Harada N et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor. Science 1993; 262: 1880-1883.