Binding of the Escherichia coli MelR protein to the melAB promoter: Orientation of MelR subunits and investigation of MelR-DNA contacts

Molecular Microbiology

Volumn 48, Issue 2, 2003, Pages 335-348

  Grainger, David C ^a   Belyaeva, Tamara A ^a   Lee, David J ^a   Hyde, Eva I ^a   Busby, Stephen J W ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CYSTEINE; EDETIC ACID DERIVATIVE; GENE PRODUCT; HELIX LOOP HELIX PROTEIN; MELIBIOSE; PROTEIN MELAB; PROTEIN MELR; PROTEIN SUBUNIT; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; DNA CLEAVAGE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN ENGINEERING; PROTEIN FAMILY; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN TARGETING; TRANSCRIPTION INITIATION; TRANSCRIPTION INITIATION SITE;

AMINO ACID MOTIFS; BASE SEQUENCE; DNA; DNA FOOTPRINTING; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MELIBIOSE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; SYMPORTERS; TRANS-ACTIVATORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0038687706     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2003.t01-1-03434.x     Document Type: Article

References (33)

1. Belyaeva, T., Wade, J., Webster, C., Howard, V., Thomas, M., Hyde, E., and Busby, S. (2000) Transcription activation at the Escherichia coli melAB promoter: the role of MelR and the cyclic AMP receptor protein. Mol Microbiol 36: 211-222.
2. Bhende, P., and Egan, S. (1999) Amino acid-DNA contacts by RhaS: an AraC transcription activator. J Bacteriol 181: 5185-5192.
3. Bhende, P., and Egan, S. (2000) Genetic evidences that transcription activation by RhaS involves specific amino acid contacts with sigma 70. J Bacteriol 182: 4959-4969.
4. Bourgerie, S., Michán, C., Thomas, M., Busby, S., and Hyde, E. (1997) DNA binding and DNA bending by the MelR transcription activator protein. Nucleic Acids Res 25: 1685-1693.
5. 70 subunit by a transcription activator. J Bacteriol 182: 2982-2984.
6. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72: 248-254.
7. Datwyler, S., and Meares, C. (2000) Protein-protein interactions mapped by artificial proteases: where σ factors bind to RNA polymerase. Trends Biol Sci 25: 408-414.
8. Egan, S. (2002) Growing repertoire of AraC/XylS activators. J Bacteriol 184: 5529-5532.
9. Gallegos, M.-T., Schleif, R., Bairoch, A., Hofmann, K., and Ramos, J.-L. (1997) AraC/XylS family of transcriptional regulators. Microbiol Mol Biol Rev 61: 393-410.
10. Ghosaini, L., Brown, A., and Sturtevant, J. (1988) Scanning calorimetric study of the thermal unfolding of catabolite activator protein from Escherichia coli: the absence and presence of cyclic mononucleotides. Biochemistry 27: 5257-5261.
11. Greiner, D., Miyake, R., Moran, J., Jones, A., Negishi, T., Ishihama, A., and Meares, C. (1997) Synthesis of the protein cutting reagent Iron (S)-1-(p-bromoacetamidobenzyl) ethylendediaminetetraacetate and conjugation to cysteine side chains. Bioconj Chem 8: 44-48.
12. Griffith, K., and Wolf, R. (2002) A comprehensive alanine scanning mutagenesis of the Escherichia coli transcriptional activator SoxS: identifying amino acids important for DNA binding and transcription activation. J Mol Biol 322: 237-257.
13. Howard, V., Belyaeva, T., Busby, S., and Hyde, E. (2002) DNA binding of the transcription activator protein MelR from Escherichia coli and its C-terminal domain. Nucleic Acids Res 30: 2692-2700.
14. Ishihama, A. (2000) Molecular anatomy of RNA polymerase using protein-conjugated metal probes with nuclease and protease activities. Chem Commun 2000: 1091-1094.
15. Kelsall, A., Evans, C., and Busby, S. (1985) A plasmid vector that allows fusion of the E. coli galactokinase gene to the translation startpoint of other genes. FEBS Lett 180: 155-159.
16. Kwon, H., Bennik, M., Demple, B., and Ellenberger, T. (2000) Crystal structure of the Escherichia coli Rob transcription factor in complex with DNA. Nature Struct Biol 7: 424-430.
17. Lodge, J., Fear, J., Busby, S., Gunasekaran, P., and Kamini, N. (1992) Broad-host-range plasmids carrying the E. coli lactose and galactose operons. FEMS Lett 95: 271-276.
18. Martin, R., and Rosner, J. (2001) The AraC transcriptional activators. Curr Opin Microbiol 4: 132-137.
19. Martin, R., Gillette, W., Rhee, S., and Rosner, J. (1999) Structural requirements for marbox function in transcription activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter. Mol Microbiol 34: 431-441.
20. Michán, C., Busby, S., and Hyde, E. (1995) The Escherichia coli MelR transcription activator: production of a stable fragment containing the DNA-binding domain. Nucleic Acids Res 23: 1518-1523.
21. Miller, J. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
22. Murakami, K., Kimura, M., Owens, J., Meares, C., and Ishihama, A. (1997) The two α subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact different halves of the DNA upstream element. Proc Natl Acad Sci USA 94: 1709-1714.
23. Niland, P., Huhne, R., and Muller-Hill, B. (1996) How AraC interacts specifically with its target DNAs. J Mol Biol 264: 667-674.
24. 70 in an Escherichia coli RNA polymerase-/acUV5 open promoter complex. Biochemistry 37: 7670-7675.
25. Reeder, T., and Schleif, R. (1993) AraC protein can activate transcription from only one position and when pointed in only one direction. J Mol Biol 231: 205-218.
26. Rhee, S., Martin, R., Rosner, J., and Davies, D. (1998) A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator. Proc Natl Acad Sci USA 95: 10413-10418.
27. Savery, N., Belyaeva, T., and Busby, S. (1996) Introduction to Protein: DNA interactions, DNase I footprinting, hydroxyl radical footprinting, permanganate footprinting and supplementary protocols. In Essential Techniques: Gene Transcription. Docherty, K. (ed.). Colchester, UK: BIOS Scientific Publishers. Chap. 1, pp. 1-5 and 21-33.
28. Savery, N., Lloyd, G., Kainz, M., Gaal, T., Ross, W., Ebright, R., et al. (1998) Transcription activation at Class II CRP-dependent promoters: identification of determinants in the C-terminal domain of the RNA polymerase alpha subunit. EMBO J 17: 3439-3447.
29. Studier, W. (1991) Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J Mol Biol 219: 37-44.
30. Wade, J., Belyaeva, T., Hyde, E., and Busby, S. (2001) A simple mechanism for co-dependence on two activators at an Escherichia coli promoter: the role of MelR and the cyclic AMP receptor protein. EMBO J 20: 7160-7167.
31. Webster, C., Kempsell, K., Booth, I., and Busby, S. (1987) Organisation of the regulatory region of the Escherichia coli melibiose operon. Gene 59: 253-263.
32. Webster, C., Gardner, L., and Busby, S. (1989) The E. coli melR gene encodes a DNA binding protein with affinity for specific sequences located in the melibiose-operon regulatory region. Gene 83: 207-213.
33. Williams, J., Michán, C., Webster, C., and Busby, S. (1994) Interactions between the E coli MelR transcription activator protein and operator sequences at the melAB promoter. Biochem J 300: 757-763.