Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells

Methods in Molecular Biology

Volumn 215, Issue , 2003, Pages 387-395

  Tsuji, Kohichiro ^a   Ueda, Takahiro ^a   Ebihara, Yasuhiro ^a  

^a THE UNIVERSITY OF TOKYO   (Japan)

Author keywords

Mouse Bone Marrow Cell; Severe Combine Immune Deficiency; Soybean Lecithin; Stem Cell Activity; Stem Cell Factor

Indexed keywords

CD34 ANTIGEN; CYTOKINE;

ANIMAL; ARTICLE; CELL DIVISION; CELL SEPARATION; CULTURE TECHNIQUE; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; METABOLISM; METHODOLOGY; MOUSE; MOUSE MUTANT; NONOBESE DIABETIC MOUSE; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

ANIMALS; ANTIGENS, CD34; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL SEPARATION; CYTOKINES; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; MICE; MICE, INBRED NOD; MICE, SCID; POLYMERASE CHAIN REACTION;

EID: 0038687285     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-345-3:387     Document Type: Chapter

References (17)

1. – cells with preserved ability to engraft SCID-hu bone. Blood 91, 1206–1215.
2. Kobayashi, M., Laver, J. H., Kato, T., Miyazaki, H., and Ogawa, M. (1996) Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3. Blood 88, 429–436.
3. Sui, X., Tsuji, K., Tanaka, R., Tajima, S., Muraoka, K., Ebihara, Y., et al. (1995) gp130 and c-Kit signallings synergize for ex vivo expansion of human primitive hematopoietic progenitor cells. Proc. Natl. Acad. Sci. USA 92, 2859–2863.
4. Yoshida, M., Tsuji, K., Ebihara, Y., Muraoka, K., Tanaka, R., Miyazaki, H., et al. (1996) Thrombopoietin alone stimulates the early proliferation and survival of human eryhtroid, myeloid and multipotential progenitors in serum-free culture. Br. J. Haematol. 98, 254–264.
5. Larochelle, A., Vormoor, J., Helmut, H., Wang, J. C., Bhatia, M., Lapidot, T., Moritz, T., et al. (1996) Identification of primitive human hematopoieitc cells capable of repopulating NOD/SCID mouse bone marrow: implications of gene therapy. Nat. Med. 2, 1329–1337.
6. Bhatia, M., Wang, J.C., Kapp, U., Bonnet, D., and Dick, J. E. (1997) Purification of primitive human hematopoietic cells capable of repopulating immune-deficient mice. Proc. Natl. Acad. Sci. USA 94, 5320–5325.
7. Gan, O. I., Murdoch, B., Larochelle, A., and Dick, J. E. (1997) Differential maintenance of primitive human SCID-repopulating cells, clonogenic progenitors, and long-term culture-initiating cells after incubation on human bone marrow stromal cells. Blood 90, 641–650.
8. Shultz, L. D., Schweitzer, P. A., Chritianson, S. W., Gott, B., Schweitzer, I. B., Tennet, B., et. al. (1997) Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J. Immunol. 154, 180–191.
9. Pflumio, F., Izac, B., Katz, A., Shultz, L. D., Vainchenker, W., and Coulombel, L. (1996) Phenotype and function of human hematopoietic cells engrafting immune-deficient CB17-severe combined immunodeficiency mice and nonobese diabetic-severe combined immnodeficiency mice after transplantation of human cord blood mononuclear cells. Blood 88, 3731–3740.
10. + adult bone marrow cells. Int. J. Hematol. 73, 457–462.
11. Ueda, T., Tsuji, K., Yoshino, H., Ebihara, Y., Yagasaki, H., Hisakawa, H., et al. (2000) Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6 and soluble IL-6 receptor. J. Clin. Invest. 105, 1013–1021.
12. Ebihara, Y., Tsuji, K., Lyman, S. D., Sui, X., Yoshida, M., Muraoka, K., et al. (1997) Synergistic action of Flt3 and gp130 signalings in human hematopoiesis. Blood 90, 4363–4368.
13. Bhatia, M., Bonnet, D., Kapp, U., Wang, J. C. Y., Murdoch, B., and Dick, J. E. (1997) Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture. J. Exp. Med. 186, 619–624.
14. Conneally, E., Cashman, J., Petzer, A., and Eaves, C. (1997) Expansion in vitro of transplantable human cord blood stem cells demonstrated using a quantitative assay of their lympho-myeloid repopulating activity in nonobese diabetic-scid/scid mice. Proc. Natl. Acad. Sci. USA 94, 9836–9841.
15. Yoshino, H., Ueda, T., Kawahata, M., Kobayashi, K., Ebihara, Y., Manabe, A., et al. (2000) Natural killer cell depletion by anti-asialo GM1 antiserum treatment enhances human hematopoietic stem cell engraftment in NOD/Shi-scid mice. Bone Marrow Transplant 26, 1211–1216.
16. Habu, S., Fukui, H., Shimamura, K., Kasai, M., Nagai, Y., Okumura, K., et al. (1981) In vitro effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice. J. Immunol. 127, 34–38.
17. Ueda, T., Yoshino, H., Kobayashi, K., Kawahata, M., Ebihara, Y., Ito, M., et al. (2000) Hematopoietic repopulating ability of cord blood CD34+ cells in NOD/ Shi-scid mice. Stem Cells 18, 204–213.