Transformation of Mucor miehei results in plasmid deletion and phenotypic instability

FEMS Microbiology Letters

Volumn 224, Issue 1, 2003, Pages 101-106

  Monfort, Aurelia ^a   Cordero, Laura ^a   Maicas, Sergi ^b   Polaina, Julio ^a  

^a INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^b UNIVERSIDAD CEU CARDENAL HERRERA   (Spain)

Author keywords

Aspartic protease; Fungal transformation geneticin resistance; Mucor circinelloides; Mucor miehei

Indexed keywords

ANTIBIOTIC G 418; PLASMID DNA;

ARTICLE; FUNGAL DETECTION; FUNGAL GENETICS; GENE DELETION; GENETIC TRANSFORMATION; MOLECULAR BIOLOGY; MUCOR; MYCELIUM; NONHUMAN; PHENOTYPE; PLASMID; PRESSURE; PRIORITY JOURNAL; PROTOPLAST; RHIZOBIUM;

AGROBACTERIUM; MUCOR; MUCOR CIRCINELLOIDES; MUCOR MIEHEI; RHIZOMUCOR MIEHEI;

EID: 0038647036     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00421-X     Document Type: Article

References (38)

1. Foltman, B. (1987) General and molecular aspects of rennets. In: Cheese: Chemistry, Physics and Microbiology, Vol. 1: General Aspects (Fox, P.F., Ed.), pp. 33-62. Elsevier Applied Science, London, New York.
2. Gray G.L., Hayenga K., Cullen D., Wilson L.J., Norton S. Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate. Gene. 48:1986;41-53.
3. Boel E., Huge-Jensen B., Christensen M., Thim L., Fiil N.P. Rhizomucor miehei lipase is synthesized as a precursor. Lipids. 23:1988;701-706.
4. Brady L., Brzozowski A.M., Derewenda Z.S., Dodson E., Dodson G., Tolley S., Turkenburg J.P., Christiansen L., Huge-Jensen B., Norskov L. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature. 343:1990;767-770.
5. Derewenda Z.S., Derewenda U., Dodson G.G. The crystal and molecular structure of Rhizomucor miehei triacylglyceride lipase at 1.9 Å resolution. J. Mol. Biol. 227:1992;818-839.
6. Yang J., Teplyakov A., Quail W. Crystal structure of the aspartic proteinase from Rhizomucor miehei at 2.15 Å resolution. J. Mol. Biol. 268:1997;449-459.
7. van Heeswijck R., Roncero M.I.G. High frequency transformation of Mucor with recombinant plasmid DNA. Carlsberg Res. Commmun. 49:1984;691-702.
8. van Heeswijck R. Autonomous replication of plasmids in Mucor transformants. Carlsberg Res. Commun. 51:1986;433-443.
9. Arnau J., Jepsen L.P., Strøman P. Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides. Curr. Genet. 225:1991;193-198.
10. Anaya N., Roncero M.I.G. Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild-type gene. Mol. Gen. Genet. 239:1991;449-455.
11. Iturriaga E.A., Díaz-Mínguez J.M., Benito E.P., Alvárez M.I., Eslava A.P. Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leu gene. Curr. Genet. 21:1992;215-223.
12. Benito E.P., Díaz-Minguez J.M., Iturriaga E.A., Campuzano V., Eslava A.P. Cloning and sequence analysis of the Mucor circinelloides pyrG gene encoding orotidine-5′-monophosphate decarboxylase. Use of pyrG for homologous transformation. Gene. 116:1992;59-67.
13. Benito E.P., Campuzano V., López-Matas M.A., De Vicente J.I., Eslava A.P. Isolation, characterization and transformation by autonomous replication of Mucor circinelloides OMPdecase-deficient mutants. Mol. Gen. Genet. 248:1995;126-135.
14. Arnau J., Strøman P. Gene replacement and ectopic integration in the zygomycete Mucor circinelloides. Curr. Genet. 23:1993;542-546.
15. Maicas S., Adam A.C., Polaina J. The ribosomal DNA of the Zygomycete Mucor miehei. Curr. Genet. 37:2000;412-419.
16. Hoekema A., Hirsch P.R., Hooykaas P.J.J., Schilperoort R.A. A binary plant vector strategy based on separation of vir and T region of the Agrobacterium tumefaciens Ti-plasmid. Nature. 303:1983;179-180.
17. Koncz C., Schell J. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector. Mol. Gen. Genet. 204:1986;383-396.
18. Hood E.E., Gelvin S.B., Melchers L.S., Hoekema A. New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2:1993;208-218.
19. Short J.M., Fernández J.M., Sorge J.A., Huse W.D. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 16:1988;7583-7600.
20. Alting-Mees M.A., Short J.M. PBlueSript II: Gene mapping vectors. Nucleic Acids Res. 17:1989;9494.
21. Yanish-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;568-572.
22. Jimenez A., Davies J. Expression of transposable antibiotic resistance elements in Saccharomyces cerevisiae. Nature. 287:1980;869-871.
23. Wach A., Brachat A., Phöhlmann R., Philippsen P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1994;1793-1808.
24. Revuelta J.L., Jayaram M. Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid. Proc. Natl. Acad. Sci. USA. 83:1986;7344-7347.
25. Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12:1984;8711-8721.
26. Hellens R.P., Edwards E.A., Leyland N.R., Bean S., Mullineaux P.M. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 42:2000;819-832.
27. Hooykaas P.J.J., Roobol C., Schilperoort R.A. Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens. J. Gen. Microbiol. 110:1979;99-109.
28. Bundock P., den Dulk-Ras A., Beijersbergen A., Hooykaas P.J.J. Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J. 114:1995;3206-3214.
29. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) In: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. van Heeswijck R. The formation of protoplasts from Mucor species. Carlsberg Res. Commun. 49:1984;597-609.
31. de Groot M.J.A., Bundock P., Hooykaas P.J.J., Beijersbergen A.G.M. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat. Biotechnol. 16:1998;839-842.
32. Thakur M.S., Karanth N.G., Nand K. Production of fungal rennet by Mucor miehei using solid state fermentation. Appl. Microbiol. Biotechnol. 32:1990;409-413.
33. An, G., Ebert, P.R., Mitra, A. and Ha, S.B. (1988) Binary vectors. In: Plant Molecular Biology Manual (Gelvin, S.B., Schilperoort, R. and Verma, D.P., Eds.), A3, pp. 1-19. Kluwer Academic Publishers, Dordrecht.
34. Suárez T., Eslava A.P. Transformation of Phycomyces with a bacterial gene for kanamycin resistance. Mol. Gen. Genet. 212:1988;120-123.
35. Fincham J.R.S. Transformation in fungi. Microbiol. Rev. 53:1989;148-170.
36. Mackenzie D.A., Wongwathanarat P., Carter A.T., Archer D.B. Isolation and use of a homologous histone H4 promoter and a ribosomal DNA region in a transformation vector for the oil-producing fungus Mortierella alpina. Appl. Environ. Microbiol. 66:2000;4655-4661.
37. Adam A.C., Prieto J.A., Rubio-Texeira M., Polaina J. Construction of a lactose-assimilating strain of baker's yeast. Yeast. 15:1999;1299-1305.
38. Cerdá-Olmedo E. Phycomyces and the biology of light and color. FEMS Microbiol. Rev. 25:2001;503-512.