A miniaturized, parallel, serially diluted immunoassay for analyzing multiple antigens

Journal of the American Chemical Society

Volumn 125, Issue 18, 2003, Pages 5294-5295

  Jiang, Xingyu ^a   Ng, Jessamine M K ^a   Stroock, Abraham D ^a   Dertinger, Stephan K W ^a   Whitesides, George M ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ANTIGEN DETECTION; ARTICLE; CONCENTRATION RESPONSE; DIAGNOSTIC VALUE; ELECTRONICS; IMMUNOASSAY; LIQUID; PROTEIN IMMOBILIZATION; QUANTITATIVE ANALYSIS; SERIAL DILUTION FLUORESCENT IMMUNOASSAY; VOLUMETRY;

ANTIBODIES; ANTIGENS; FLUOROIMMUNOASSAY; HIV ANTIBODIES; IMMUNOGLOBULIN G; MICROCHEMISTRY; MINIATURIZATION; SERUM ALBUMIN, BOVINE;

EID: 0038631842     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja034566+     Document Type: Article

References (17)

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4. Stroock, A. D.; Dertinger, S. K.; Ajdari, A.; Mezic, I.; Stone, H. A.; Whitesides, G. M. Science 2002, 295, 647-651. There are other designs of mixers; this μDN should work with any appropriate mixer, for example: Liu, R. H.; Stremler, M. A.; Sharp, K. V.; Olsen, M. G.; Santiago, J. G.; Adrian, R. J.; Aref, H.; Beebe, D. J. J. Microelectromech. Syst. 2000, 9, 190-197. Rohr, T.; Yu, C.; Davey, M. H.; Svec, F.; Frechet, J. M. J. Electrophoresis 2001, 22, 3959-3967. Thorsen, T.; Maerkl, S. J.; Quake, S. R. Science 2002, 298, 580-584.
5. Stroock, A. D.; Dertinger, S. K.; Ajdari, A.; Mezic, I.; Stone, H. A.; Whitesides, G. M. Science 2002, 295, 647-651. There are other designs of mixers; this μDN should work with any appropriate mixer, for example: Liu, R. H.; Stremler, M. A.; Sharp, K. V.; Olsen, M. G.; Santiago, J. G.; Adrian, R. J.; Aref, H.; Beebe, D. J. J. Microelectromech. Syst. 2000, 9, 190-197. Rohr, T.; Yu, C.; Davey, M. H.; Svec, F.; Frechet, J. M. J. Electrophoresis 2001, 22, 3959-3967. Thorsen, T.; Maerkl, S. J.; Quake, S. R. Science 2002, 298, 580-584.
6. Stroock, A. D.; Dertinger, S. K.; Ajdari, A.; Mezic, I.; Stone, H. A.; Whitesides, G. M. Science 2002, 295, 647-651. There are other designs of mixers; this μDN should work with any appropriate mixer, for example: Liu, R. H.; Stremler, M. A.; Sharp, K. V.; Olsen, M. G.; Santiago, J. G.; Adrian, R. J.; Aref, H.; Beebe, D. J. J. Microelectromech. Syst. 2000, 9, 190-197. Rohr, T.; Yu, C.; Davey, M. H.; Svec, F.; Frechet, J. M. J. Electrophoresis 2001, 22, 3959-3967. Thorsen, T.; Maerkl, S. J.; Quake, S. R. Science 2002, 298, 580-584.
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8. This technology is used for the assays of many diseases that elicit production of antibodies in bodily fluids, for example: Wang, J. J.; Steel, S.; Wisniewolski, R.; Wang, C. Y. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 6159-6163. Wreghitt, T. G.; Morgan-Capner, P. ELISA in the Clinical Microbiology Laboratory; Public Health Laboratory Service: London, 1990.
9. This technology is used for the assays of many diseases that elicit production of antibodies in bodily fluids, for example: Wang, J. J.; Steel, S.; Wisniewolski, R.; Wang, C. Y. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 6159-6163. Wreghitt, T. G.; Morgan-Capner, P. ELISA in the Clinical Microbiology Laboratory; Public Health Laboratory Service: London, 1990.
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15. Other groups have reported comparable values for these antibodies in HIV+ serum, for example: Binley, J. M.; Klasse, P. J.; Cao, Y.; Jones, I.; Markowitz, M.; Ho, D. D.; Moore, J. P. J. Virol. 1997, 71, 2799-2809.
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