Detection of viable toxigenic Vibrio cholerae and virulent Shigella spp. in environmental waters by pit-stop seminested polymerase chain reaction assays

Water SA

Volumn 29, Issue 2, 2003, Pages 177-182

  Du Preez, M ^a   Venter, S N ^b   Theron, J ^b  

^a DSI CSIR Nanotechnology Innovation Centre   (South Africa)

^b UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

Detection; Drinking water; Environmental waters; Polymerase chain reaction; Shigella; Vibrio cholerae; Water borne pathogens

Indexed keywords

DNA; ELECTROPHORESIS; ENVIRONMENTAL IMPACT; GENES; POTABLE WATER; TOXIC MATERIALS;

POLYMERASE CHAIN REACTIONS (PCR);

BIOASSAY;

BACTERIUM; DETECTION METHOD; DRINKING WATER; WATER QUALITY;

BACTERIA (MICROORGANISMS); SHIGELLA; VIBRIO; VIBRIO CHOLERAE;

EID: 0038518237     PISSN: 03784738     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. ARROYO G and ARROYO JA (1996) Selective action of inhibitors used in different culture media on competetive microflora of Salmonella. J. Appl. Bateriol. 78 281-289.
2. BEJ AK, MAHBUBANI MH, DICESARE JL and ATLAS RM (1991) Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Appl. Environ. Microbiol. 57 3529-3534.
3. BOBB CA, SUMMER JW, MORRIS GK and WELLS JG (1981) Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium. J. Clin. Microbiol. 13 714-719.
4. BYRD JJ, XU H-S and COLWELL RR (1991) Viable but nonculturable bacteria in drinking-water. Appl. Environ. Microbiol. 57 875-878.
5. CASH RA, MUSIC SI, LIBONATI JP, SNYDER MJ, WENTZEL RP and HORNICK RB (1974) Response of man to infection with Vibrio cholerae. Clinical, serologic and bacteriologic responses to a known inoculum. J. Infect. Dis. 129 45-52.
6. CERDÀ-CUÉLLAR M, JOFRE J and BLANCH AR (2000) A selective medium and a specific probe for detection of Vibrio vulnificus. Appl. Environ. Microbiol. 66 855-859.
7. DEPAOLA A and HWANG G (1995) Effect of dilution, incubation time, and temperature of enrichment on cultural and PCR detection of Vibrio cholerae obtained from the oyster Crassostrea virginica. Mol. Cell Probes 9 75-81.
8. DEPARTMENT OF PROVINCIAL AND LOCAL GOVERMENT (2001) National Status Report on Cholera Epidemic in South Africa (http://sandmc.pwv.gov.za/ndmc/cholera/).
9. DE RYCK R, STRUELENS MJ and SERRUYS EJ (1994) Rapid biochemical screening for Salmonella, Shigella, Yersinia and Aeromonas isolates from stool specimens. J. Clin. Microbiol. 32 1583-1585.
10. DONOVAN TJ and VAN NETTEN P (1995) Culture media for the enumeration of pathogenic Vibrio species in foods and environmental samples. Int. J. Food Microbiol. 26 77-91.
11. FARMER JJ and HICKMAN-BRENNER FW (1992) The Genera Vibrio and Photobacterium. In: Balows A, Trüper HG, Dworkin M, Harder W and Schleifer K-H (eds.) The Prokaryotes pp. 2952-3011. Springer-Verlag, New York.
12. FRAHM E and OBST U (1995) Comparison of two rapid tests for the detection of Legionellaceae in water: A microbiological-immunological method and a commercial gene-probing testing. Water Sci. Technol. 31 403-406.
13. GONZALEZ AT, TORRES J, DVORSKY E and TORANZOS GA (1995) Modified culture methods for the detection of Vibrio spp. from estuarine waters. Water Sci. Technol 31 283-290.
14. GRACZYCK TK, CRANFIELD MR and FAYER R (1997) Recovery of waterborne oocysts of Cryptosporidium from water samples by the membrane-filter dissolution method. Parasitol. Res. 83 121-125.
15. HØI L, DALSGAARD I and DALSGAARD A (1998) Improved isolation of Vibrio vulnificus from seawater and sediment with Cellobiose-Colistin agar. Appl. Environ. Microbiol. 64 1721-1724.
16. JOSEPHSON KL, GERBA CP and PEPPER IL (1993) Polymerase chain reaction of nonviable bacterial pathogens. Appl. Environ. Microbiol. 59 3513-3515.
17. JUCK D, INGRAM J, PRÉVOST M, COALLIER J and GREER C (1996) Nested PCR protocol for the rapid detection of Escherichia coli in potable water. Can. J. Microbiol. 42 862-866.
18. KANG DH and SIRAGUSA GR (1999) Agar underlay method for recovery of sublethally heat-injured bacteria. Appl. Environ. Microbiol. 65 5334-5337.
19. KREADER, CA (1996) Relief on amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl. Environ. Microbiol. 62 1102-1106.
20. LINDQVIST R (1999) Detection of Shigella spp. in food with a nested PCR method - sensitivity and performance compared with a conventional culture method. J. Appl. Microbiol 86 971-978.
21. McDONALD IR, KENNA EM and MURRELL JC (1995) Detection of methanotrophic bacteria in environmental samples with the PCR. Appl. Environ. Microbiol. 61 116-121.
22. NILSSON L, OLIVER ID and KJELLEBERG S (1991) Resuscitation of Vibrio vulnificus from the viable but nonculturable state. J. Bacteriol. 173 5054-5059.
23. OYOFO BA and ROLLINS DM (1993) Efficacy of filter types for detecting Campylobacter jejuni and Campylobacter coli in environmental water samples by polymerase chain reaction. Appl. Environ. Microbiol. 59 4090-4095.
24. PALMER CJ, TSAI Y-L, PASZKO-KOLVA C, MAYER C and SANGERMANO LR (1993) Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods. Appl. Environ. Microbiol. 59 3618-3624.
25. PEGRAM GC, ROLLINS N and ESPREY Q (1998) Estimating the costs of diarrhoea and epidemic dysentery in KwaZulu-Natal and South Africa. Water SA 24 11-20.
26. REISSBRODT R, HEIER H, TSCHÄPE H, KINGSLEY RA and WILLIAMS PH (2000) Resuscitation by ferrioxamine E of stressed Salmonella enterica serovar typhimurium from soil and water microcosms. Appl. Environ. Microbiol. 66 4128-4130.
27. ROWE B and GROSS RJ (1984) Facultatively anaerobic gram negative rods. Genus II. Shigella. In: Krieg NR and Holt JG (eds.) Bergey's Manual of Systematic Bacteriology pp. 423-427. Williams & Wilkins, Baltimore, MD.
28. SMIBERT RM and KRIEG NR (1994) Phenotypic characterisation. In: Gerhardt P, Murray RGE, Wood WA and Krieg NR (eds.) General and Molecular Bacteriology pp. 614-651. American Society for Microbiology, Washington DC.
29. th edn) American Public Health Association, Washington DC.
30. THERON J, CILLIERS J, DU PREEZ M, BRÖZEL VS and VENTER SN (2000) Detection of toxigenic Vibrio cholerae from environmental water samples by an enrichment broth cultivation-pit-stop seminested PCR procedure. J. Appl. Microbiol. 89 539-546.
31. THERON J, MORAR D, DU PREEZ M, BRÖZEL VS and VENTER SN (2001) A sensitive seminested PCR method for the detection of Shigella in spiked environmental water samples. Water. Res. 35 869-875.
32. TORO A, GONZALES N, TORRES J, DVORSKY E and TORANZOS GA (1995) Modified culture methods for detectionof Vibrio spp. from estuarine water. Water. Sci. Technol. 31 283-290.
33. WEAVER JW and ROWE MT (1997) Effect of non-target cells on the sensitivity of the PCR for Escherichia coli O157:H7. Lett. Appl Microbiol. 25 109-112.
34. WILSON IG (1997) Inhibition and facilitation iof nucleic acid amplification. Appl. Environ. Microbiol. 63 3741-3751.
35. YOKOMAKU D, YAMAGUCHI N and NASU M (2000) Improved direct viable count procedure for quantitative estimation of bacterial viability in freshwater environments. Appl. Environ. Microbiol. 66 5544-5548.