Role of the Bacillus methanolicus citrate synthase II gene, citY, in regulating the secretion of glutamate in L-lysine-secreting mutants

Applied and Environmental Microbiology

Volumn 69, Issue 7, 2003, Pages 3986-3995

  Brautaset, Trygve ^a   Williams, Mark D ^b   Dillingham, Richard D ^b   Kaufmann, Christine ^b   Bennaars, Assumpta ^b   Crabbe, Edward ^b   Flickinger, Michael C ^b  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTORS; FERMENTATION; GENES; MUTAGENESIS; PROTEINS;

THERMOTOLERANCE;

BACTERIA;

AMMONIA; CARBON; CITRATE SYNTHASE; GLUTAMIC ACID; LYSINE; METHANOL; OXOGLUTARATE DEHYDROGENASE; PYRUVATE DECARBOXYLASE;

MICROBIOLOGY;

ARTICLE; BACILLUS; BACILLUS METHANOLICUS; BACTERIAL GENE; BACTERIUM MUTANT; BIOREACTOR; ENZYME ACTIVITY; ESCHERICHIA COLI; FED BATCH REACTOR; FERMENTATION; FRAMESHIFT MUTATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; CITRATE (SI)-SYNTHASE; CLONING, MOLECULAR; CULTURE MEDIA; GENE EXPRESSION REGULATION, BACTERIAL; GLUTAMIC ACID; LYSINE; METHANOL; MOLECULAR SEQUENCE DATA; MUTATION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

BACILLUS METHANOLICUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA; PROKARYOTA; TRIXIS;

EID: 0038492851     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.7.3986-3995.2003     Document Type: Article

References (56)

1. Ackermann, J.-U., and W. Babel. 1994. Glutamic acid synthesis form methanol: theoretical considerations. J. Basic Microbiol. 4:211-216.
2. Anthony, C. 1991. Assimilation of carbon by methylotrophs, p. 79-109. In I. Goldberg and J. S. Rokem (ed.), Biology of methylotrophs. Butterworth-Heinemann, Boston, Mass.
3. Arfman, N., E. M. Watling, W., Clement, R. J. van Oosterwijk, G. E. de Vries, W. Harder, M. M. Attwood, and L. Dijkhuizen. 1989. Methanol metabolism in thermotolerant methylotrophic Bacillus strains involving a novel catabolic NAD-dependent methanol dehydrogenase as a key enzyme. Arch. Microbiol. 152:280-288.
4. Arfman, N., L. Dijkhuizen, G. Kirchhof, W. Ludwig, K.-H. Schleifer, E. S. Bulygina, K. M. Chumakov, N. I. Govorukhina, Y. A. Totsenko, D. White, and R. J. Sharp. 1992. Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria. Int. J. Syst. Bacteriol. 42:439-445.
5. Belitsky, B. R. 2002. Biosynthesis of amino acids of the glutamate and aspartate families, alanine, and polyamines, p. 203-231. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and its closest relatives. ASM Press, Washington, D.C.
6. Cnovas, J. L., and H. L. Kornberg. 1969. Phosphoenol pyruvate carboxylase from Escherichia coli. Methods Enzymol. 13:288-292.
7. Chistoserdova, L., L. Gomelsky, J. A. Vorholt, M. Gemelsky, Y. D. Tsyganov, and M. Lidstrom. 2000. Analysis of the formaldehyde oxidation pathways in Methylobacillus flagellatus KT, a ribulose monophosphate cycle methylotroph. Microbiology 146:233-238.
8. Connis, H., S. M. West, D. W. Hough, and M. J. Danson. 1998. Cloning and overexpression in Escherichia coli of the gene encoding citrate synthase from the hyperthermophilic archaeon Sulfolobus solfataricus. Extremophiles 2:61-66.
9. Cue, D., H. Lam, R. L. Dillingham, R. S. Hanson, and M. C. Flickinger. 1997. Genetic manipulation of Bacillus methanolicus, a gram-positive thermotolerant methylotroph. Appl. Environ. Microbiol. 63:1406-1420.
10. Danson, M. J., and D. W. Hough. 1998. Structure, function and stability of enzymes from the Archaea. Trends. Microbiol. 6:307-315.
11. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
12. De Vries, G. E., U. Kües, and U. Stahl. 1990. Physiology and genetics of methylotrophic bacteria. FEMS Microbiol. Rep. 75:57-102.
13. De Vries, G. E., N. Arfman, P. Terpstra, and L. Dijkhuizen. 1992. Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene. J. Bacteriol. 174:5346-5353.
14. Dijkhuizen, L., P. R. Levering, and G. E. de Vries. 1992. The physiology and biochemistry of aerobic methanol-utilizing gram negative and gram positive bacteria, p. 149-181. In J. C. Murrell and H. Dalton (ed.), Methane and methanol utilizers. Plenum Press, New York, N.Y.
15. Donohue, T. J., and D. A. Bernlohr. 1978. Carbon and nitrogen catabolite repression, metabolite pools, and the regulation of sporulation in Bacillus licheniformis, p. 293-298. In G. Chambliss and J. C. Vary (ed.), Spores VII. American Society for Microbiology, Washington, D.C.
16. Eikmanns, B. J., N. Thum-Schmitz, L. Eggeling, K. U. Ludtke, and H. Sahm. 1994. Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase. Microbiology 140:1817-1828.
17. Gerike, U., D. W. Hough, N. J. Russell, M. L. Dyall-Smith, and M. J. Danson. 1998. Citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships. Microbiology 144:929-935.
18. 1 compounds. Kluywer Academic Publishers, Dordrecht, The Netherlands.
19. Hodgson, J. A., P. N. Lowe, and R. N. Perham. 1983. Wild type and mutant forms of pyruvate dehydrogenase multienzyme complex from Bacillus subtilis. Biochem. J. 211:463-472.
20. Jagusztyn-Krynicki, E. K., and A. Malashewska-Keough. 1989. Cloning and expression of Thiobacillus versutus aspartate-semialdehyde dehydrogenase gene in E. coli. FEMS Microbiol. Lett. 59:21-26.
21. Jeong, J. W., J. Snay, and M. M. Ataai. 1990. Mathematical model for examining growth and sporulation processes of Bacillus subtilis. Biotechnol. Bioeng. 25:160-184.
22. Jin, S., and A. L. Sonenshein. 1994. Identification of two distinct Bacillus subtilis citrate synthase genes. J. Bacteriol. 176:4669-4679.
23. Jin, S., and A. L. Sonenshein. 1994. Transcriptional regulation of Bacillus subtilis citrate synthase genes. J. Bacteriol. 176:4680-4690.
24. Jin, S., and A. L. Sonenshein. 1996. Characterization of the major citrate synthase of Bacillus subtilis. J. Bacteriol. 178:3658-3660.
25. Johnson, D. E., and R. S. Hanson. 1974. Bacterial citrate synthase: purification, molecular weight and kinetic mechanism. Biochim. Biophys. Acta 350:336-353.
26. Jourlin-Castelli, C., N. Mani, M. M. Nakano, and A. L. Sonensheim. 2000. CcpC, a novel regulator of the LysR family required for glucose repression of the citB gene in Bacillus subtilis. J. Mol. Biol. 295:865-878.
27. Kuramitsu, S., K. Hiromo, H. Hayashi, Y. Morino, and H. Kagamiyama. 1990. Pre-steady-state kinetics of Escherichia coli aspartate aminotransferase catalyzed reactions and thermodynamic aspects of its substrate specificity. Biochemistry 29:5469-5476.
28. Kurz, L. C., G. Drysdale, M. Riley, M. A. Tomar, J. Chen, R. J. Russell, and M. J. Danson. 2000. Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum. Biochemistry 39:2283-2296.
29. Lee, G. H., W. Hur, C. E. Bremmon, and M. C. Flickinger. 1996. L-lysine production from methanol at 50°C with Bacillus methanolicus: modeling volume control, L-lysine concentration, and productivity with a three-phase continuous simulation. Biotechnol. Bioeng. 49:639-653.
30. Libor, S. M., T. K. Sundaram, and M. C. Scrutton. 1978. Pyruvate carboxylase from a thermophilic Bacillus. Biochem. J. 169:543-558.
31. Marchenko, G. N., N. D. Marchenko, Y. D. Tsyganov, and A. Y. Chistoserdov. 1999. Organization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus. Microbiology 145:3273-3282.
32. Mills, D. A., and M. C. Flickinger. 1992. Cloning and sequencing of the meso-diaminopimelate decarboxylase gene from Bacillus methanolicus MGA3 and comparison to other decarboxylase genes. Appl. Environ. Microbiol. 59:2927-2937.
33. Motoyama, H., K. Maki, H. Anazawa, S. Ishino, and S. Teshiba. 1994. Cloning and nucleotide sequences of the homoserine dehydrogenase genes (hom) and the threonine synthase genes (thrC) of the gram-negative obligate methylotroph Methylobacillus flagellatus. Appl. Environ. Microbiol. 60:111-119.
34. Mukherjee, B. B., J. Matthews, D. L. Horney, and L. J. Reed. 1965. Resolution and reconstitution of Escherichia coli α-ketoglutarate dehydrogenase complex. J. Biol. Chem. 240:2268-2269.
35. Nakayama, K., M. Kobata, Y. Tanaka, T. Nomura, and R. Katsumata. 1975. Verfahren zur biotechnischen Herstellung von L-Glutaminsäure und Mikroorgansimen zur Durchführung des Verfahrens. DE 2438206.
36. Paulus, H. 1993. Biosynthesis of the aspartate family of amino acids, p. 237-267. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria. American Society for Microbiology, Washington, D.C.
37. Peters-Wendisch, P. G., B. Schiel, V. F. Wendisch, E. Katsoulidis, B. Mockel, H. Sahm, and B. J. Eikmanns. 2001. Pyruvate carboxylase is a major bottleneck for glutamate and L-lysine production by Corynebacterium glutamicum. J. Microbiol. Biotechnol. 3:295-300.
38. Pluschkell, S. B. 1999. Characterization and mathematical modeling of growth and glutamic acid production by Bacillus methanolicus MGA3. Ph.D. thesis, University of Minnesota, Minneapolis, Minn.
39. 13C NMR and isotope ratio mass spectrometry. Microbiology 148:3223-3233.
40. Reed, L. J., and B. B. Mukherjee. 1969. α-Ketoglutarate dehydrogenase complex from Escherichia coli. Methods Enzymol. 13:55-61.
41. Renner, E. D., and R. W. Bernlohr. 1972. Characterization and regulation of pyruvate carboxylase of Bacillus licheniformis. J. Bacteriol. 213:53-59.
42. Russell, R. J. M., U. Gerike, M. J. Danson, D. W. Hough, and G. L. Taylor. 1998. Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium. Structure 6:351-361.
43. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
44. Schendel, F. J., C. E. Bremmon, M. C. Flickinger, M. Guettler, and R. S. Hanson. 1990. L-Lysine production at 50°C by mutants of a newly isolated and characterized Bacillus sp. Appl. Environ. Microbiol. 56:963-970.
45. Schendel, F. J., P. R. August, C. R., Andersen, R. S. Hanson, and M. C. Flickinger. 1992. Cloning and nucleotide sequence of the gene coding for citrate synthase from a thermotolerant Bacillus sp. Appl. Environ. Microbiol. 58:335-345.
46. Schendel, F. J., and M. C. Flickinger. 1992. Cloning and nucleotide sequence of the gene encoding aspartokinase II from a thermophilic methylotrophic Bacillus sp. Appl. Environ. Microbiol. 58:2806-2814.
47. Schreier, H. J., T. M. Smith, T. J. Donohue, and R. W. Bernlohr. 1981. Regulation of nitrogen metabolism and sporulation in Bacillus licheniformis, p. 138-141. In H. S. Levinson, A. L. Sonenshein, and D. J. Tipper (ed.), Sporulation and germination. American Society for Microbiology, Washington, D.C.
48. Seubert, W., and H. Weicker. 1969. Pyruvate carboxylase from Pseudomonas. Methods Enzymol. 13:258-262.
49. Shrishinka, V. N., and Y. A. Trotsenko. 1982. Multiple enzymic lesions in obligate methanotrophic bacteria. FEMS Microbiol. Lett. 13:237-242.
50. Sonenshein, A. L. 2002. The Krebs citric acid cycle, p. 151-162. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and its closest relatives. ASM Press, Washington, D.C.
51. Stackebrandt, E., and B. M. Gobel. 1994. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846-849.
52. Takami, H., K. Nakasone, Y. Takaki, G. Maeno, R. Sasaki, N. Masui, F. Fuji, C. Hirama, Y. Nakamura, N. Ogasawara, S. Kuhara, and K. Horikoshi. 2000. Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis. Nucleic Acids Res. 28:4317-4331.
53. Tobisch, S., D. Zulcke, J. Bernhardt, J. Stulke, and M. Hecker. 1999. Role of CcpA in regulation of central pathways of carbon catabolism in Bacillus subtilis. J. Bacteriol. 181:6996-7004.
54. Vorholt, J. A., L. Chistoserdova, S. M. Stolyar, R. K. Thauer, and M. Lidstrom. 1999. Distribution of tetrahydrometanopterin-dependent enzymes in methylotrophic bacteria and phylogeny of methenyl tetrahydrometanopterin cyclohydrolases. J. Bacteriol. 181:5750-5757.
55. Yagi, T., H. Kagamiyama, M. Nozaki, and K. Soda. 1985. Glutamate-aspartate transaminase from microorganism. Methods Enzymol. 113:83-89.
56. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103-119.