Catalytic mechanism of Escherichia coli isopentenyl diphosphate isomerase involves Cys-67, Glu-116, and Tyr-104 as suggested by crystal structures of complexes with transition state analogues and irreversible inhibitors

Journal of Biological Chemistry

Volumn 278, Issue 14, 2003, Pages 11903-11908

  Wouters, J ^a,b   Oudjama, Y ^a   Barkley, Sam J ^c   Tricot, C ^a   Stalon, V ^a,b   Droogmans, L ^b   Poulter, C Dale ^c  

^a Inst de Rech Microbiol JM Wiame   (Belgium)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^c UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CATALYSIS; CRYSTAL STRUCTURE; ESCHERICHIA COLI; ISOMERIZATION;

DEPROTONATION;

ENZYMES;

3,4 EPOXY 3 METHYL 1 BUTYL DIPHOSPHATE; CYSTEINE; DIMETHYLALLYL DIPHOSPHATE ISOMERASE; ENZYME INHIBITOR; GLUTAMIC ACID; ISOMERASE; ISOPENTENYL DIPHOSPHATE DELTA ISOMERASE; ISOPRENOID; N,N DIMETHYL 2 AMINO 1 ETHYL DIPHOSPHATE; PYROPHOSPHORIC ACID DERIVATIVE; TYROSINE; UNCLASSIFIED DRUG; 3 METHYL 3,4 EPOXYBUTYL DIPHOSPHATE; 3-METHYL-3,4-EPOXYBUTYL DIPHOSPHATE; EPOXIDE; ISOPENTENYLDIPHOSPHATE DELTA-ISOMERASE; ORGANOPHOSPHORUS COMPOUND;

ARTICLE; BIOSYNTHESIS; COMPLEX FORMATION; COVALENT BOND; CRYSTAL STRUCTURE; ENZYME INACTIVATION; ENZYME MECHANISM; ESCHERICHIA COLI; ISOMERISM; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; STRUCTURE ANALYSIS; CHEMISTRY; CRYSTALLOGRAPHY; DRUG ANTAGONISM; ENZYME ACTIVE SITE; ENZYMOLOGY; KINETICS; METABOLISM; PROTEIN SECONDARY STRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

CARBON-CARBON DOUBLE BOND ISOMERASES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY; CYSTEINE; EPOXY COMPOUNDS; ESCHERICHIA COLI; GLUTAMIC ACID; KINETICS; ORGANOPHOSPHORUS COMPOUNDS; PROTEIN STRUCTURE, SECONDARY; TYROSINE;

EID: 0038485617     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M212823200     Document Type: Article

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