PCR-DGGE and PCR-RFLP analyses of the internal transcribed spacer (ITS) of ribosomal DNA in the genus Rhizopus

Journal of Microbiology

Volumn 41, Issue 2, 2003, Pages 157-160

  Park, You Jung ^a   Choi, Yong Keel ^b   Min, Byung Re ^a  

^a Sangmyung University   (South Korea)

^b Hanyang University   (South Korea)

Author keywords

DGGE; Internal transcribed spacer (ITS); PCR RFLPs; Rhizopus

Indexed keywords

INTERNAL TRANSCRIBED SPACER; RESTRICTION ENDONUCLEASE; RIBOSOME DNA;

AMPLICON; ARTICLE; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; ENZYMATIC DEGRADATION; FUNGAL STRAIN; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC POLYMORPHISM; GENETIC VARIABILITY; HAPLOTYPE; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RHIZOPUS; RHIZOPUS HOMOTHALLICUS; RHIZOPUS MICROSPORUS; RHIZOPUS ORYZAE; RHIZOPUS STOLONIFER; STRAIN DIFFERENCE;

MICROSPORUS; RHIZOPUS; RHIZOPUS HOMOTHALLICUS; RHIZOPUS MICROSPORUS; RHIZOPUS ORYZAE; RHIZOPUS STOLONIFER;

EID: 0038458421     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Dweika, I.M., H. Ohm, S. Mackenzie, F. Patterson, S. Cambron, and R. Ratcliffe. 1994. Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Teor. Appl. Genet. 89, 964-968.
2. Ellis, D.H. 1981. Sporangiospore ornamentation of thermophilic Rhizopus species and some allied genera. Mycologia. 73, 511-523.
3. Ellis, J.J. 1985. Species and varieties in the Rhizopus arrhizus-Rhizopus oryzae group as indicated by their DNA complementarily. Mycologia. 77, 243-247.
4. Hesseltine, C.W. and J.J. Ellis. 1973. Mucorales, p. 187-217. In G.C. Ainsworth, F.K. Sparrow., and A.S. Sussman (eds.), Academic Press. New York and London.
5. Inui, T., Y. Takeda., and H. Iizuka. 1965. Taxonomical studies on genus Rhizopus. J. Gen. Appl. Microbiol. Suppl. 11, 1-121.
6. Kowalchuk, G.A., S. Gerards, and J.W. Woldendorp. 1997. Detection and characterization of fungal infections of Ammophila arenaria (Marram Grass) roots by denturing gradient gel electrophoresis of specifically amplified 18S rDNA. Appl. Environ. Microbiol. 63, 3853-3865.
7. Lee, Y.-M., Y.K. Choi, and B.R. Min. 2000. PCR-RFLP and sequence analysis of the rDNA ITS region in the Fusarium spp. Kor. J. Microbiol. 38, 66-73.
8. Lessa, E.P. 1992. Rapid surveying of DNA sequence variation in natural populations. Mol. Biol. Evol. 9, 323-330.
9. Myers, R.M., S.G. Fischer, L.S. Lerman, and T. Maniatis. 1985a. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res. 13, 3131-3145.
10. Myers, R.M., S.G. Fischer, T. Maniatis, and L.S. Leman. 1985b. Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis. Nucleic Acids Res. 13, 3111-3129.
11. Myers, R.M., T. Maniatis, and L.S. Lerman. 1987. Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol, 155, 501-527.
12. Paul, B. 2001. ITS region of the rDNA of Pythium longandrum, a new species; its taxonomy and its comparison with related species. FEMS Microbiol. Lett. 202, 239-242.
13. Schipper, M.A.A and J.A. Stalpers. 1984. Studies in Mycology. No 25 p. 1-34. In M.A.A. Schipper and J.A. Stalpers (eds.), A revision of the genus Rhizopus, Institute of the Royal Netherlands, Academy of Science and Letters.
14. Scholer, J.J. 1970. Mucormykosenbei Mensch und Tier. Taxomoie der Erreger. Chemotherapie im Tierexperiment und in der Klinik. Habilschr., Basel.
15. Siboe, G.M., M. Johanne, and M.K. Paul. 2000. Genetic similarity among Cercospora apii-group species and their detection in host plant tissue by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS). J. Gen. Appl. Microbiol. 46, 69-78.
16. White, T.J., T.D. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal genes form phylogenetics, p. 315-322. In M.A. Innis, D.H. Gelfrand, J.J. Sninsky, and T.J. White (eds.), Academic Press, San Diego, California.