Genetic similarity among Cercospora apii-group species and their detection in host plant tissue by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS)

Journal of General and Applied Microbiology

Volumn 46, Issue 2, 2000, Pages 69-78

  Siboe, George M ^a   Murray, Johanne ^b   Kirk, Paul M ^c  

^a UNIVERSITY OF NAIROBI   (Kenya)

^b UNIVERSITY OF SUSSEX   (United Kingdom)

^c CABI   (United Kingdom)

Author keywords

Cercospora; ITS PCR; Pseudocercospora; RDNA; RFLP

Indexed keywords

AGAROSE; DNA FRAGMENT; PLANT DNA; RESTRICTION ENDONUCLEASE; RIBOSOME DNA; SPACER DNA;

AGRICULTURE; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; ELECTROPHORESIS; FUNGAL GENETICS; FUNGUS; FUNGUS CULTURE; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; GENOME; HOST; INFECTION CONTROL; MYCOSIS; NONHUMAN; PLANT LEAF; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SCREENING; SPINACH; STRAIN DIFFERENCE;

CERCOSPORA; CERCOSPORA APII; CERCOSPORA BETICOLA; CERCOSPORA CARICIS; FUNGI; PSEUDOCERCOSPORA; PSEUDOCERCOSPORA CAVARAE; PSEUDOCERCOSPORA CONYZAE; PSEUDOCERCOSPORA PURPUREA; SPINACIA OLERACEA;

EID: 0033841572     PISSN: 00221260     EISSN: None     Source Type: Journal    
DOI: 10.2323/jgam.46.69     Document Type: Article

References (45)

1. RDNA: Evolution over a billion years
2. Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction
3. DNA polymorphism in morels: PCR-RFLP analysis of the ribosomal DNA spacers and microsatellite-primed PCR
4. Species-specific polymorphism in transcribed ribosomal DNA of five Pythium species
5. Species delimitation in the African Armillaria complex by analysis of the rDNA spacers
6. Amplification of fungal rDNA - Its regions from non-fertile specimens of the lichen-forming genus Parmelia
7. Studies on Cercospora and allied genera. V. Mycovellosiella Rangel and a new species of Ramulariopsis
8. Studies on Cercospora and allied genera
9. Studies on Cercospora and allied genera. VII. New species and redisposition
10. Molecular drive: A cohesive model of species evolution
11. Restriction analysis of ITS and the small subunit gene of ribosomal DNA among four Gaeumannomyces species
12. Use of Random Amplified Polymorphic DNA (RAPD) for identification of Gaeumannomyces species
13. ITS-RFLP matching for identification of fungi
14. A PCR based identification method for species of Armillaria
15. Typing truffle species by PCR amplification of ribosomal DNA spacers
16. Ribosomal RNA and fungal systematics
17. Synonymy in some species of Cercospora
18. Purple stain of soybean seeds incited by several Cercospora species
19. Detection of Pythium ultimum using polymerase chain reaction with species-specific primers
20. Inter- and intra-specific variation in the ITS region of rDNA of ectomycorrhizal fungi in Fennoscandia as detected by endonuclease analysis
21. The significance of ITS-RFLPs and coenzyme Q system in determining taxonomic relationships among Candida species
22. Single-strand conformation polymorphism analysis of PCR-amplified rDNA internal transcribed spacers to differentiate species of Aspergillus sect. Flavi
23. Phylogeny of five fungus-like protoctistan Phytophthora species, inferred from the internal transcribed spacers of ribosomal DNA
24. PCR detection, identification to species level, and finger-printing of Campylobacter jejuni and Campylobacter coli direct from diarrheic sample
25. Typing with ITS regions of P. carinii from AIDS patients with recurrent pneumonia
26. Virulence of Rhizoctonia oryzae and R. solani AG-8 on wheat and detection of R. oryzae in plant tissues by PCR
27. Intergenic transcribed spacer PCR ribotyping for differentiation of Saccharomyces species and interspecific hybrids
28. The classification of isolates of Gaeumannomyces graminis from wheat, rye and oats using restriction fragment length polymorphisms in families of repeated DNA sequences
29. Characterization and identification of black alder ectomycorrhizas by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS)
30. PCR amplification of ribosomal DNA for species identification in the plant pathogen genus Phytophthora
31. Ribosomal DNA sequence analysis reveals new species groupings in the genus Colletotrichum
32. Morphological and biochemical inter-relationships among isolates of Cercospora from Kenya
33. A proposed species concept in the genus Cercospora Fres. with reference to Kenyan isolates
34. Morphology and pathogenicity of Cercospora apii f. nicotianae
35. Restriction fragment length polymorphisms in isolates of Aspergillus fumigatus probed with part of the intergenic spacers region from the ribosomal RNA genes complex of Aspergillus nidulans
36. Selective amplification of rDNA internal transcribed spacer region to detect Ophiosphaerella korrae and O. herpotricha
37. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species
38. Identification of fungi in the Gaeumannomyces-Phialophora complex by RFLPs of PCR-amplified ribosomal DNAs
39. Frequency and timing of fungicide applications for the control of gray leaf spot in maize
40. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics