Fusion deficiency induced by mutations at the dimer interface in the Newcastle disease virus hemagglutinin-neuraminidase is due to a temperature-dependent defect in receptor binding

Journal of Virology

Volumn 77, Issue 12, 2003, Pages 6913-6922

  Corey, Elizabeth A ^a   Mirza, Anne M ^a   Levandowsky, Elizabeth ^a,b   Iorio, Ronald M ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b WORCESTER POLYTECHNIC INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; DIMER; HN PROTEIN; MEMBRANE PROTEIN; MONOMER; MUTANT PROTEIN; PROTEIN SUBUNIT; SIALIC ACID; VIRUS FUSION PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CHIMERA; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE MUTATION; HEMADSORPTION; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; MUTATIONAL ANALYSIS; NEWCASTLE DISEASE PARAMYXOVIRUS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE; X RAY CRYSTALLOGRAPHY;

ANIMALS; CELL LINE; CRICETINAE; DIMERIZATION; GIANT CELLS; HN PROTEIN; MEMBRANE FUSION; MODELS, MOLECULAR; MUTATION; NEWCASTLE DISEASE VIRUS; PROTEIN CONFORMATION; RECEPTORS, VIRUS; TEMPERATURE;

EID: 0038279845     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.12.6913-6922.2003     Document Type: Article

References (26)

1. Connaris, H., T. Takimoto, R. Russell, S. Crennell, I. Moustafa, A. Portner, and G. Taylor. 2002. Probing the sialic acid binding site of the hemagglutinin-neuraminidase of Newcastle disease virus: identification of key amino acids involved in cell binding, catalysis, and fusion. J. Virol. 76:1816-1824.
2. Crennell, S., T. Takimoto, A. Portner, and G. Taylor. 2000. Crystal structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase. Nat. Struct. Biol. 7:1068-1074.
3. Deng, R., Z. Wang, R. L. Glickman, and R. M. Iorio. 1994. Glycosylation within an antigenic site on the HN glycoprotein of Newcastle disease virus interferes with its role in the promotion of membrane fusion. Virology 204:17-26.
4. Deng, R., Z. Wang, P. J. Mahon, M. Marinello, A. M. Mirza, and R. M. Iorio. 1999. Mutations in the NDV HN protein that interfere with its ability to interact with the homologous F protein in the promotion of fusion. Virology 253:43-54.
5. Deng, R., Z. Wang, A. M. Mirza, and R. M. Iorio. 1995. Localization of a domain on the paramyxovirus attachment protein required for the promotion of cellular fusion by its homologous fusion protein spike. Virology 209:457-469.
6. Fuerst, T. R., E. G. Niles, F. W. Studier, and B. Moss. 1986. Eucaryotic transient expression system based on recombinant vaccinia virus virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 83:8122-8126.
7. Iorio, R. M., J. B. Borgman, R. L. Glickman, and M. A. Bratt. 1986. Genetic variation within a neutralizing domain on the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. J. Gen. Virol. 67:1393-1403.
8. Iorio, R. M., and M. A. Bratt. 1983. Monoclonal antibodies to Newcastle disease virus: delineation of four epitopes on the HN glycoprotein. J. Virol. 48:440-450.
9. Iorio, R. M., G. M. Field, J. M. Sauvron, A. M. Mirza, R. Deng, P. J. Mahon, and J. Langedijk. 2001. Structural and functional relationship between the receptor recognition and neuraminidase activities of the Newcastle disease virus hemagglutinin-neuraminidase protein: receptor recognition is dependent on neuraminidase activity. J. Virol. 75:1918-1927.
10. Iorio, R. M., R. L. Glickman, A. M. Riel, J. P. Sheehan, and M. A. Bratt. 1989. Functional and neutralization profile of seven overlapping antigenic sites on the HN glycoprotein of Newcastle disease virus: monoclonal antibodies to some sites prevent viral attachment. Virus Res. 13:245-262.
11. Iorio, R. M., R. J. Syddall, R. L. Glickman, A. M. Riel, J. P. Sheehan, and M. A. Bratt. 1989. Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus. Virology 173:196-204.
12. Iorio, R. M., R. J. Syddall, J. P. Sheehan, M. A. Bratt, R. L. Glickman, and A. M. Riel. 1991. Neutralization map of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: domains recognized by monoclonal antibodies that prevent receptor recognition. J. Virol. 65:4999-5006.
13. Lamb, R. A., and D. Kolakofsky. 1996. Paramyxoviridae: the viruses and their replication, p. 1305-1340. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.), Field's virology, 4th ed. Lippincott-Raven, Philadelphia, Pa.
14. Mahon, P. J., R. Deng, A. M. Mirza, and R. M. Iorio. 1995. Cooperative neuraminidase activity in a paramyxovirus. Virology 213:441-444.
15. Mirza, A. M., R. Deng, and R. M. Iorio. 1994. Site-directed mutagenesis of a conserved hexapeptide in the paramyxovirus hemagglutinin-neuraminidase glycoprotein: effects on antigenic structure and function. J. Virol. 68:5093-5099.
16. Mirza, A. M., J. P. Sheehan, L. W. Hardy, R. L. Glickman, and R. M. Iorio. 1993. Structure and function of a membrane anchorless form of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. J. Biol. Chem. 268:21425-21431.
17. Morrison, T. G., and A. Portner. 1991. Structure, function and intracellular processing of the glycoproteins of Paramyxoviridae, p. 347-382. In D. W. Kingsbury (ed.), The paramyxoviruses. Plenum, New York, N.Y.
18. Murrell, M., M. Porotto, T. Weber, O. Greengard, and A. Moscona. 2003. Mutations in human parainfluenza virus type 3 hemagglutinin-neuraminidase causing increased receptor binding activity and resistance to the transition state sialic acid analog 4-GU-DANA (zanamivir). J. Virol. 77:309-317.
19. Nussbaum, O., C. C. Broder, and E. A. Berger. 1994. Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by novel recombinant vaccinia virus virus-based assay quantitating cell fusion-dependent reporter gene activation. J. Virol. 68:5411-5422.
20. Scheid, A., and P. W. Choppin. 1973. Isolation and purification of the envelope proteins of Newcastle disease virus. J. Virol. 11:263-271.
21. Scheid, A., and P. W. Choppin. 1974. Identification and biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475-490.
22. Sheehan, J. P., R. M. Iorio, R. J. Syddall, R. L. Glickman, and M. A. Bratt. 1987. Reducing agent-sensitive dimerization of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus correlates with the presence of cysteine at residue 123. Virology 161:603-606.
23. Takimoto, T., G. L. Taylor, H. C. Connaris, S. J. Crennell, and A. Portner. 2002. Role of the hemagglutinin-neuraminidase protein in the mechanism of paramyxovirus-cell membrane fusion. J. Virol. 76:13028-13033.
24. Tanabayashi, K., and R. W. Compans. 1996. Functional interaction of paramyxovirus glycoproteins: identification of a domain in HN that promotes cell fusion. J. Virol. 70:6112-6118.
25. Thompson, S. D., W. G. Laver, K. G. Murti, and A. Portner. 1988. Isolation of a biologically active soluble form of the hemagglutinin-neuraminidase protein of Sendai virus. J. Virol. 62:4653-4660.
26. Tsurodome, M., M. Kawano, T. Yuasa, N. Tabata, M. Nishio, H. Komada, and Y. Ito. 1995. Identification of regions on the hemagglutinin-neuraminidase protein of human parainfluenza virus type 2 important for promoting cell fusion. Virology 213:190-203.