A modified method for the determination of plasma total homocysteine by high performance liquid chromatography

Turkish Journal of Medical Sciences

Volumn 33, Issue 2, 2003, Pages 71-76

  Tokgöz, Serhat ^a   Bilgi, Cumhur ^a   Çakir, Erdinç ^a   Erbil, M Kemal ^a   Kutluay, Türker ^a  

^a GULHANE SCHOOL OF MEDICINE   (Turkey)

Author keywords

ABD F; High performance liquid chromatography; Homocysteine; Mercaptoethanol; Sulfamoylbenzofurazan

Indexed keywords

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PLASMAS; REDUCTION;

PLASMA PROTEINS;

PROTEINS;

7 FLUORO 2,1,3 BENZOXADIAZOLE 4 SULFONAMIDE; BENZOFURAN DERIVATIVE; BUFFER; HOMOCYSTEINE; MERCAPTOETHANOL; METHANOL; PHOSPHATE; PLASMA PROTEIN; SULFAMOYLBENZOFURAZAN; SULFONAMIDE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ACCURACY; ADULT; AMINO ACID BLOOD LEVEL; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DERIVATIZATION; FEMALE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MALE; REPRODUCIBILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY; SEX DIFFERENCE; SPECTRUM;

EID: 0038248831     PISSN: 13000144     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Jacobsen DW. Homocysteine and vitamins in cardiovascular disease. Clin Chem 44: 1833-43, 1998.
2. Allen RH, Stabler SP, Savage DG, Lindenbaum J. Diagnosis of cobalamin deficency I: Usefulness of serum methylmalonic acid and total homocysteine concentrations. Am J Hematol 34: 90-8, 1990.
3. Skovby F. Homocystinuria. Clinical, biochemical and genetic aspects of cystathionine beta-synthase and its deficiency in man. Acta Paediatr Scand 321: 1-21, 1985.
4. Kang SS, Wong PWK, Malinow MR. Hyperhomocyst(e)inemia a sa risk factor for occlusive vascular disease. Annu Rev Nutr 12: 279-98, 1992.
5. Okhura Y, Nohta H. Fluorescence derivatization in high-performance liquid chromatography. Adv Chromatogr 29: 221-58, 1989.
6. Refsum H, Helland S, Ueland PM. Radioenzymic determination of homocysteine in plasma and urine. Clin Chem 31: 624-28, 1985.
7. Stabler SP, Marcell PD, Podell ER, Allen RH. Quantitation of total homocysteine, total cysteine, and methionine in normal serum and urine using capillary gas chromatography-mass spectrometry. Anal Biochem 162: 185-96, 1987.
8. Zighetti ML, Chantarangkul V, Tripodi A, Mannucci PM, Cattaneo M. Determination of total homocysteine in plasma: comparison of the Abbott Imx immunoassay with high performance liquid chromatography. Haematologica 87: 89-94, 2002.
9. Garcia AJ, Apitz-Castro R. Plasma total homocysteine quantification: an improvement of the classical high-performance liquid chromatographic method with fluorescence detection of the thiol-SBD derivatives. J Chromatogr B 779: 359-63, 2002
10. Baeyens W, van der Weken G, Lin Ling B, de Moerloose P. HPLC determination of N-acetylcysteine in pharmaceutical preparations after precolumn derivatization with thiolyte MB using fluorescence detection. Anal Lett 21: 741-57, 1988.
11. Jacobsen DW, Gatautis VJ, Green R, Robinson K, Savon SR, Secic M, Ji J, Otto JM, Taylor LM. Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: Sex differences and correlation with cobalamin and folate concentrations in healthy subjects. Clin Chem 40: 873-81, 1994.
12. Vester B, Rasmussen K. High performance liquid chromatography method for rapid and accurate determination of homocysteine in plasma and serum. Eur J Clin Chem Clin Biochem 29: 549-54, 1991.
13. Ueland PM, Refsum H, Stabler SP, Malinow MR, Andersson A. Total homocysteine in plasma or serum: Methods and clinical applications. Clin Chem 39: 1764-79, 1993.
14. Imai K, Uzu S, Toyooka T. Fluorogenic reagents, having benzofurazan structure, in liquid chromatography. J Pharm Biomed Anal 7: 1395-1403, 1990.
15. Ling BL, Dewaele C, Baeyens WRG. Micro liquid chromatography with fluorescence detection of thiols and disuphides. J Chromatogr 553: 433-49, 1991.
16. Kang SS. Treatment of hyperhomocysteinemia: Physiological basis. J Nutr 126: 1273-5, 1996.
17. Ueland PM, Refsum H. Plasma homocysteine, a risk factor for vascular disease: Plasma levels in health, disease, and drug therapy. J Lab Clin Med 114: 473-501, 1989.
18. Refsum H, Ueland PM, Svardal AM. Fully automated fluorescence assay for determining total homocysteine levels in plasma. Clin Chem 35: 1921-7, 1989.
19. Lussier CS, Xhignesse M, Piolot A, Selhub J, Davignon J, Genest J. Plasma total homocysteine in healthy subjects: sex specific relation with biological traits. Am J Clin Nutr 64: 587-93, 1996.
20. Refsum H, Helland S, Ueland PM. Fasting plasma homocysteine as a sensitive parameter to antifolate effect. A study on psoriasis patients receiving low-dose methotrexate treatment. Clin Pharmacol Ther 46: 510-20, 1989.