Stereospecific production of the herbicide phosphinothricin (Glufosinate): Purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process

Applied and Environmental Microbiology

Volumn 62, Issue 10, 1996, Pages 3794-3799

  Bartsch, Klaus ^a   Schneider, Rudolf ^a   Schulz, Arno ^a  

^a SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRATE AMINOTRANSFERASE; 4 METHYLTHIO 2 OXOBUTYRIC ACID; ASPARTATE AMINOTRANSFERASE; ASPARTIC ACID; GLUTAMIC ACID; HERBICIDE; PHOSPHINOTHRICIN; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; CONTROLLED STUDY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR CLONING; NONHUMAN; SEQUENCE HOMOLOGY; STEREOSPECIFICITY; THERMOPHILIC BACTERIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0029782672     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.10.3794-3799.1996     Document Type: Article

References (19)

1. Alfano, J. R., and M. L. Kahn. 1993. Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti. J. Bacteriol. 175:4186-4196.
2. Bartsch, K., R. Dichmann, P. Schmitt, E. Uhlmann, and A. Schulz. 1990. Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: cloning, characterization, and overexpression of the gene encoding a phosphinothricin-specific transaminase from Escherichia coli. Appl. Environ. Microbiol. 56:7-12.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Chen, E. Y., and P. H. Seeburg. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165-170.
5. Cubellis, M. V., C. Rozzo, G. Nitti, M. I. Arnone, G. Marino, and G. Sannia, 1989. Cloning and sequencing of the gene coding for aspartate aminotransferase from the thermoacidophilic archaebacterium Sulfolobus solfataricus. Eur. J. Biochem. 186:375-381.
6. Kubarski, S., T. Kasberg, and J. Distler. 1994. The nusG gene of Streptomyces griseus: cloning of the gene and analysis of the A-factor binding properties of the gene product. FEMS Microbiol. Lett. 119:33-39.
7. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
8. Meade, H. M., S. R. Long, G. B. Ruvkun, S. E. Brown, and F. M. Ausubel. 1982. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J. Bacteriol. 149:114-122.
9. Rastogi, V. K., and R. J. Watson. 1991. Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti. J. Bacteriol. 173:2879-2887.
10. 9a. Rozell, J. D. May 1989. U.S. patent 4826766.
11. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
12. Schulz, A., P. Taggeselle, D. Tripier, and K. Bartsch. 1990. Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli. Appl. Environ. Microbiol. 56:1-6.
13. Sung, M.-H., K. Tanizawa, Y. Masu, H. Tanaka, and K. Soda. 1988. Bacterial aspartate aminotransferase: its occurrence in thermophilic bacteria, and the isolation and identification of new thermophiles. Agric. Biol. Chem. 52:269-270.
14. Sung, M.-H., K. Tanizawa, H. Tanaka, S. Kuramitsu, H. Kagamiyama, K. Hirotsu, A. Okamoto, T. Higuchi, and K. Soda. 1991. Thermostable aspartate aminotransferase from a thermophilic Bacillus species. J. Biol. Chem. 266:2567-2572.
15. Sung, M.-H., K. Tanizawa, H. Tanaka, S. Kuramitsu, H. Kagamiyama, and K. Soda. 1990. Purification and characterization of thermostable aspartate aminotransferase from a thermophilic Bacillus species. J. Bacteriol. 172: 1345-1351.
16. Tanaka, T., S. Yamamoto, T. Moriya, M. Taniguchi, H. Hayashi, H. Kagamiyama, and S. Oi. 1994. Aspartate aminotransferase from a thermophilic formate-utilizing methanogen, Methanobacterium thermoformicicum strain SF-4: relation to serine and phosphoserine aminotransferases, but not to the aspartate aminotransferase family. J. Biochem. 115:309-317.
17. Urdea, M. S., J. P. Merryweather, G. T. Mullenbach, D. Coid, U. Heberlein, P. Valenzuela, and J. Barr. 1983. Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast. Proc. Natl. Acad. Sci. USA 80:7461-7465.
18. Vieira, J., and J. Messing. 1982. The pUC plasmid, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 79:259-268.
19. Walter, J. F., and M. B. Sherwin. January 1986. United Kingdom patent GB2161 159A.