Development and validation of a molecular beacon probe-based real-time polymerase chain reaction assay for rapid detection of methicillin resistance in Staphylococcus aureus

Archives of Pathology and Laboratory Medicine

Volumn 127, Issue 7, 2003, Pages 845-849

  Elsayed, Sameer ^a,c   Chow, Barbara L ^c   Hamilton, Nina L ^c   Gregson, Daniel B ^a,b,c   Pitout, Johann D D ^a,c   Church, Deirdre L ^a,b,c  

^a UNIVERSITY OF CALGARY   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c CALGARY LABORATORY SERVICES   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICON; ARTICLE; ASSAY; BACTERIAL GENOME; BACTERIUM COLONY; BACTERIUM ISOLATE; COAGULASE NEGATIVE STAPHYLOCOCCUS; LABORATORY TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BACTERIAL PROTEINS; CARRIER PROTEINS; COAGULASE; ENDONUCLEASES; ENTEROBACTERIACEAE; ENTEROCOCCUS; FLUORESCENT DYES; HEXOSYLTRANSFERASES; METHICILLIN; METHICILLIN RESISTANCE; MICROCOCCAL NUCLEASE; MOLECULAR PROBES; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PENICILLIN-BINDING PROTEINS; PEPTIDYL TRANSFERASES; PROSPECTIVE STUDIES; PSEUDOMONAS AERUGINOSA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; TIME FACTORS; URBAN HEALTH SERVICES;

EID: 0038236675     PISSN: 00039985     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Ayliffe GA. The progressive intercontinental spread of methicillin-resistant Staphylococcus aureus. Clin Infect Dis. 1997;24(suppl 1):S74-S79.
2. Solberg CO. Spread of Staphylococcus aureus in hospitals: causes and prevention. Scand J Infect Dis. 2000;32:587-595.
3. Martin MA. Methicillin-resistant Staphylococcus aureus: the persistent resistant nosocomial pathogen. Curr Clin Top Infect Dis. 1994;14:170-191.
4. Boyce JM. MRSA patients: proven methods to treat colonization and infection. J Hosp Infect. 2001;48(suppl A):S9-S14.
5. Simor AE. Containing methicillin-resistant S. aureus: surveillance, control, and treatment methods. Postgrad Med. 2001;110:43-48.
6. Hospital Infection Control Practices Advisory Committee. Recommendations for preventing the spread of vancomycin resistance. Infect Control Hosp Epidemiol. 1995;16;105-113.
7. Brown DF. Detection of methicillin/oxacillin resistance in staphylococci. J Antimicrob Chemother. 2001;48(suppl 1):65-70.
8. Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS. Real-time multiplex PCR assays. Methods. 2001;25:430-442.
9. Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol. 1996;14:303-308.
10. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res. 1996;6:986-994.
11. Reischl U, Linde HJ, Metz M, Birgit L, Lehn N. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J Clin Microbiol. 2000;38:2429-2433.
12. Tan TY, Corden S, Barnes R, Cookson B. Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR. J Clin Microbiol. 2001;39:4529-4531.
13. Grisold AJ, Leitner E, Muhlbauer G, Marth E, Kessler HH. Detection of methicillin-resistant Staphylococcus aureus and simultaneous confirmation by automated nucleic acid extraction and real-time PCR. J Clin Microbiol. 2002;40:2392-2397.
14. Shrestha NK, Tuohy MJ, Hall GS, Isada CM, Procop GW. Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler System. J Clin Microbiol. 2002;40:2659-2661.
15. Katayama Y, Ito T, Hiramatsu K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother. 2000;44:1549-1555.
16. Ito T, Katayama Y, Asada K, et al. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2001;1323-1326.
17. Berger-Bachi B, Rohrer S. Factors influencing methicillin resistance in staphylococci. Arch Microbiol. 2002;178:165-171.