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-6 torr) and contained salt (EDTA, ratio of DNA to EDTA = 1:1 by weight) during electron irradiation; the latter was reported to shield the DNA toward low-energy electrons
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-6 torr) and contained salt (EDTA, ratio of DNA to EDTA = 1:1 by weight) during electron irradiation; the latter was reported to shield the DNA toward low-energy electrons.
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L. Sanche, unpublished data.
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0343203297
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note
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-3, assuming minimal clustering of the plasmids in the solid.
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17
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0026600950
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with the plasmid solids containing a mixture of A and C conformations. Removal of this intrinsic hydration water is believed to lead to substantial conformational changes and DSBs, which are not observed in our unirradiated control samples held under UHV
-
Desiccation leaves plasmid DNA with its structural water of about 2.5 water molecules per base pair [S. G. Swarts, M. D. Sevilla, D. Becker, C. J. Tokar, K. T. Wheeler, Radiat. Res. 129, 333 (1992)], with the plasmid solids containing a mixture of A and C conformations. Removal of this intrinsic hydration water is believed to lead to substantial conformational changes and DSBs, which are not observed in our unirradiated control samples held under UHV.
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Sevilla, M.D.2
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Wheeler, K.T.5
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20
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0343203296
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B. D. Michael et al., in (3), pp. 251-258
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B. D. Michael et al., in (3), pp. 251-258.
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23
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_, J. Phys. Chem. 95, 4902 (1991).
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24
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26
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0000896264
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For vertical electron attachment energies in gas-phase nucleobases below 5 eV, see K. Aflatooni, G. A. Gallup, P. D. Burrow, J. Phys. Chem. A 102, 6205 (1998); for detachment energies in solvated uracil cluster anions, see J. H. Hendricks, S. A. Lyapustina, H. L. de Clercq, K. H. Bowen, J. Chem. Phys. 108, 8 (1998).
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27
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11344270521
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For vertical electron attachment energies in gas- phase nucleobases below 5 eV, see K. Aflatooni, G. A. Gallup, P. D. Burrow, J. Phys. Chem. A 102, 6205 (1998); for detachment energies in solvated uracil cluster anions, see J. H. Hendricks, S. A. Lyapustina, H. L. de Clercq, K. H. Bowen, J. Chem. Phys. 108, 8 (1998).
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Hendricks, J.H.1
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29
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0003397857
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Plenum, New York
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30
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0342768677
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Sample exposure-response curves can be seen at Science Online
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Sample exposure-response curves can be seen at Science Online (www.sciencemag.org/feature/data/1044957.shl).
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31
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0342768676
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note
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We thank A. J. Waker for helpful comments and suggestions. Supported by the Medical Research Council of Canada.
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