Generation and characterization of species-specific anti-Bcl-XL monoclonal antibodies

Hybridoma and Hybridomics

Volumn 22, Issue 2, 2003, Pages 91-95

  Hsu, Yi Te ^a   Hou, Qi ^a   Cymbalyuk, Eugene ^a   Youle, Richard ^b  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY BCL XL; PROTEIN BCL XL; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING;

ANIMALIA; MURINAE;

EID: 0038173579     PISSN: 15368599     EISSN: None     Source Type: Journal    
DOI: 10.1089/153685903321948012     Document Type: Article

References (11)

1. Boise LH, Gonzalez-Garcia M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nunez G, and Thompson CB: bcl-X, a Bcl-2 related gene that functions as a dominant regulator of apoptotic cell death. Cell 1993; 74:597-608.
2. L and Bcl-2 repress a common pathway of cell death. J Exp Med 1995;182:821-828.
3. Motoyama N, Wang F, Roth KA, Sawa H, Nakayama K-I, Nakaya K, Negishi I, Senju S, Zhang Q, Fujii S, and Loh DY: Massive cell death of immature hematopoietic cells and neurons in Bcl-X-deficient mice. Science 1995;267:1506-1510.
4. L is the major bcl-X mRNA form expressed during murine development and its product localizes to mitochondria. Development 1994;120:3033-3042.
5. L during apoptosis. Proc Natl Acad Sci USA 1997;94:3668-3672.
6. Wolter KG, Hsu Y-T, Smith CL, Nechushtan A, Xi X-G, and Youle RJ: Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 1997;139:1281-1292.
7. Muchmore SW, Sattler M, Liang H, Meadows RP, Harlan JE, Yoon HS, Nettesheim D, Chang BS, Thompson CB, Wong S-L, Ng S-C, and Fesik SW: X-ray and NMR structure of human Bcl-XL, an inhibitor of programmed cell death. Nature 1996;381:335-341.
8. Yin X-M, Oltvai ZN, and Korsmeyer SJ: BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 1994;369:321-323.
9. Huang DC, Adams JM, and Cory S: The conserved N-terminal BH4 domain of Bcl-2 homologues is essential for inhibition of apoptosis and interaction with CED-4. EMBO J 1998;17:1029-1039.
10. Hsu Y-T, and Youle RJ: Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 1997;272: 13829-13834.
11. Hsu Y-T, and Youle RY: Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J Biol Chem 1998;273:10777-10783.