Purification and characterization of alginate lyase from marine bacterium Vibrio sp. QY101

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 5, 2003, Pages 473-477

  Song, Kai ^a   Yu, Wen Gong ^a   Han, Feng ^a   Han, Wen Jun ^a   Li, Jing Bao ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Alginate; Alginate lyase; Characterization; Purification; Vibrio sp.

Indexed keywords

ALGINIC ACID; AMMONIUM SULFATE; LYASE;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIOPLANKTON; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; THERMOSTABILITY;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; MOLECULAR WEIGHT; POLYSACCHARIDE-LYASES; SUBSTRATE SPECIFICITY; TEMPERATURE; VIBRIO;

ALGAE; BACTERIA (MICROORGANISMS); PHAEOPHYCEAE; UNCULTURED MARINE BACTERIUM; VIBRIO; VIBRIO SP.;

EID: 0038149179     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Gacesa P. Alginates. Carbohydr Polym, 1988, 8: 161-182
2. Sanford PA, Baird J. Industrial utilization of polysaccharides. In: Aspinall GO ed. The Polysaccharides, Now York: Academic, 1983, 411-490
3. Sutherland IW. Novel and established applications of microbial polysaccharides. Trends Biotechnol, 1998, 16(1): 41-46
4. Heyraud A, Colin-Morel P, Gey C, Chavagnat F, Guinand M, Wallach J. An enzymatic method for preparation of homopolymennuronate blocks and strictly alternating sequences of mannuronic and guluronic units. Carbohyd Res, 1998, 308 (3-4): 417-422
5. Ochi Y, Takeuchi T, Murata K,Kawabata Y,Kusakabe I. A simple method for preparation of poly-mannuronate using polyguluronate lyase. Biosc Biotechno Biochem, 1995, 59: 1560-1561
6. Boyd A, Chakrabarty AM. Role of alginate lyase in cell detachment of Pseudomonas aeruginosa. Appl Environ Microbiol, 1994, 60(7): 2355-2359
7. Linker A, Evans LR. Isolation and characterization of an alginase from mucoid strains of Pseudomonas aeruginosa. J Bacteriol, 1984, 159(3): 958-964
8. Sadoff HL. Encystment and germination in Azotobacter vine-landii. Bacteriol Rev, 1975, 39(4): 516-539
9. Bayer AS, Speert DP, Park S, Tu J, Witt M, Nast CC, Normun DC. Functional role of mucoid exopolysaccharide (alginate) in antibiotic-induced and polymerphonuclear leukocyte-mediated killing of Pseudomonas aeruginosa. Infect Immun, 1991, 59(1): 302-308
10. Bayer AS, Park S, Ramos MC, Nast CC, Eftekhar F, Schiller NL. Effects of alginase on the natural history and antibiotic therapy of experimental endocarditis caused by mucoid Pseudomonas aeruginosa. Infect Immun, 1992, 60(10): 3979-3985
11. Eftekhar F, Speert DP. Alginase treatment of mucoid Pseudomonas aeruginosa enhances phagocytosis by human monocyte-derived macrophages. Infect Immun, 1988, 56 (11): 2788-2793
12. Fuchs HJ, Borowitz DS, Christiansen DH, Morris EM, Nash ML, Ramsey BW, Rosenstein BJ et al. Effect of aerosolized recombinant human DNase on exacerbations of respiratory symptoms and on pulmonary function in patients with cystic fibrosis. The pulmozyma study group. N Engl J Med, 1994, 331 (10): 637-642
13. Hatch RA, Schiller NL. Alginate lyase promotes diffusion of aminoglycosides through the extracellular polysaccharide of mucoid Pseudomonas aeruginosa. Antimicrob Agents Chemother, 1998, 42 (4): 974-977
14. Boyen C, Bertheau Y, Barbeyron T, KloaregB. Preparation of guluronate lyase from Pseudomonas alginovora for protoplast isolation in Laminaria. Enzyme Microb Technol, 1990, 12 (11): 885-890
15. Butler DM, Østgaard K, Boyen C, Evans LV, Jensen A, Kloareg B. Isolation conditions for high yields of protoplasts from Laminaria saccharina and L. digitata. J Exp Bot, 1989, 40 (220): 1237-1246
16. Li JW, Xiao NG, Yu RY, Yuan MX, Chen LR, then YH, Chen LT. Principles and Methods of Biochemistry Experiments. Beijing: Publishing House of Peiking University, 1994, 168-224
17. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature, 1970, 227(259): 680-685
18. Preiss J, Ashwell G. Alginic acid metabolism in bacteria. I. Enzymatic formation of unsaturated oligosaccharides and 4-deoxy-L-erythro-5-hexoseulose uronic acid. J Biol Chem, 1962, 237(2): 309-316
19. Sawabe T, Ohtsuka M, Ezura Y. Novel alginate lyases from marine bacterium Alteromonas sp. strain H-4. Carbohydr Res, 1997, 304(1): 69-76
20. Yoshiko I, Ryoko A, Ken-Ichi I, Tatsuya O, Hisataka F, Shinziro H, Tsuyoshi M. Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates. Biosci Biotechnol Biochem, 2001, 65(1): 133-142
21. Kitamikado M, Tseng CH, Yamaguchi K, Nakamura T. Two types of bacterial alginate lyases. Appl Environ Microbiol, 1992, 58(8): 2474-2478
22. Takeshita S, Sato N, Igarashi M, Muramatsu T. A highly denaturantdurable alginate lyase from a marine bacterium: Purification and properties. Biosci Biotechnol Biochem, 1993, 57(7): 1125-1128
23. Takeshita S, Oda T, Muramatsu T. Spectroscopic studies on denaturants and guluronate lyase from a marine bacterium. Biosci Biotechnol Biochem, 1995, 59: 881-885
24. Tseng CH, Yamaguchi K, Kitamikado M. Two types of alginate lyase from a marine bacterium Vibrio sp. AL-9. Nippon Suisan Gakkaishi, 1992, 58(4): 743-749
25. Nakada HI, Sweeny PC. Alginic acid degradation by eliminases from abalone hepatopancreas. J Biol Chem, 1967, 242 (5): 845-851