The structure of the helically perturbed flagellar filament of Pseudomonas rhodos: Implications for the absence of the outer domain in other complex flagellins and for the flexibility of the radial spokes

Molecular Microbiology

Volumn 48, Issue 5, 2003, Pages 1305-1316

  Cohen Krausz, Sara ^a   Trachtenberg, Shlomo ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

FLAGELLIN;

ARTICLE; BACTERIAL FLAGELLUM; ELECTRON MICROSCOPY; HYDRODYNAMICS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; PSEUDOMONAS RHODOS; RHIZOBIUM; SALMONELLA TYPHIMURIUM;

FLAGELLA; FLAGELLIN; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, ELECTRON, SCANNING; MODELS, MOLECULAR; PSEUDOMONAS;

BACTERIA (MICROORGANISMS); METHYLOBACTERIUM RHODINUM; NEGIBACTERIA; PSEUDOMONAS; RHIZOBIUM; RHIZOBIUM LUPINI; SALMONELLA TYPHIMURIUM;

EID: 0038120891     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2003.03466.x     Document Type: Article

References (50)

1. Acker, G., Schmitt, R., and Mayer, F. (1975) Elektronenmikroskopische untersuchung alternder zellen von Pseudomonas rhodos. Feinstruktur nativer und isolierter tubularer membranen. Arch Microbiol 104: 215-223.
2. Calladine, C.R. (1975) Construction of bacterial flagella. Nature 255: 121-124.
3. Calladine, C.R. (1976) Design requirements for the construction of bacterial flagella. J Theor Biol 57: 469-489.
4. Calladine, C.R. (1978) Change of waveform in bacterial flagella: the role of mechanics at the molecular level. J Mol Biol 118: 457-479.
5. Cohen-Krausz, S., and Trachtenberg, S. (1998) Helical perturbations of the flagellar filament: Rhizobium lupini H13-3 at 13 Å resolution. J Struct Biol 122: 267-282.
6. Cohen-Krausz, S., and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol 321: 383-395.
7. DeRosier, D.J., and Moore, P.B. (1970) Reconstruction of three-dimensional images from electron micrographs of structures with helical symmetry. J Mol Biol 52: 355-369.
8. Finch, J.T., and Klug, A. (1972) The helical surface lattice of bacterial flagella. In The Generation of Subcellular Structures, Vol. 1. Markham, R., Bancroft, J.B., Davies, D.R., Hopwood, D.A., and Home, R.W. (eds). Amsterdam: North Holland Publishing Co., pp. 167-177.
9. Götz, R., and Schmitt, R. (1987) Rhizobium meliloti swims by unidirectional, intermittent rotation of right-handed flagellar helices. J Bacteriol 169: 3146-3150.
10. Götz, R., Limmer, N., Ober, K., and Schmitt, R. (1982) Motility and chemotaxis in two strains of Rhizobium with complex flagella. J Gen Microbiol 128: 789-798.
11. Homma, M., DeRosier, D.J., and Macnab, RM. (1990) Flagellar hook and hook-associated proteins of Salmonella typhimurium and their relationship to other axial components of the flagellum. J Mol Biol 213: 819-832.
12. Hyman, H.C., and Trachtenberg, S. (1991) Point mutations that lock Salmonella typhimurium flagellar filaments in the straight right-handed and left-handed forms and their relation to filament superhelicity. J Mol Biol 220: 79-88.
13. Krupski, G., Götz, R., Ober, K., Pleier, E., and Schmitt, R. (1985) Structure of complex flagellar filaments in Rhizobium meliloti. J Bacteriol 1162: 361-366.
14. Kuwajima, G. (1988) Construction of a minimum-size functional flagellin of Escherichia coli. J Bacteriol 170: 3305-3309.
15. Kuwajima, G., Asaka, J.-I., Fujiwara, T., Fujiwara, T., Nakano, K., and Kondo, E. (1988) Presentation of an antigenic determinant from hen egg-white lysozyme on the flagellar filament of Escherichia coli. Biotechniques 6: 1080-1083.
16. Lotz, W., Acker, G., and Schmitt, R. (1977) Bacteriophage 7-7-1 adsorbs to the complex flagella of Rhizobium lupini H13-3. J Gen Virol 34: 9-17.
17. Lowy, J., and Hanson, J. (1965) Electron microscope studies of bacterial flagella. J Mol Biol 11: 293-313.
18. Maruyama, M., Lodderstaedt, G., and Schmitt, R. (1978) Purification and biochemical properties of complex flagella isolated from Rhizobium lupini H13-3. Biochim Biophys Acta 535: 110-124.
19. Marx, R., and Heumann, W. (1962) Ueber Geisselfeinstrukturen und Fimbrien bei zwei Pseudomonas-staemmen. Arch Mikrobiol 43: 245-254.
20. Mimori, Y., Yamashita, I., Murata, K., Fujiyoshi, Y., Yonekura, K., Toyoshima, C., and Namba, K. (1995) The structure of the R-type straight flagellar filament of Salmonella at 9 Å resolution by electron cryomicroscopy. J Mol Biol 249: 69-87.
21. Mimori-Kiyosue, Y., Yamashita, I., Fujiyoshi, Y., Yamaguchi, S., and Namba, K. (1998) Role of outermost subdomain of Salmonella flagellin in the filament structure revealed by electron cryomicroscopy. J Mol Biol 284: 521-530.
22. Morgan, D.G., Owen, C., Melanson, L.A., and DeRosier, D.J. (1995) Structure of bacterial flagellar filaments at 11 Å resolution: packing of the α-helices. J Mol Biol 249: 88-110.
23. Owen, C., and DeRosier, D.J. (1993) A 13 Å map of the actin-scruin filament from the Limulus acrosomal process. J Cefi Biol 123: 337-344.
24. Owen, C., Morgan, D.G., and DeRosier, D.J. (1996) Image analysis of helical objects: the Brandeis helical package. J Struct Biol 116: 167-175.
25. Platzer, J., Sterr, W., Hausmann, M., and Schmitt, R. (1997) Three genes of a motility operon and their role in flagellar rotary speed variation in Rhizobium meliloti. J Bacteriol 179: 6391-6399.
26. Pleier, E., and Schmitt, R. (1989) Identification and Sequence analysis of two related flagellin genes in Rhizobium meliloti. J Bacteriol 171: 1467-1475.
27. Pleier, E., and Schmitt, R. (1991) Expression of two Rhizobium meliloti flagellin genes and their contribution to the complex filament structure. J Bacteriol 173: 2077-2085.
28. Raska, I., Mayer, F., Edelbluth, C., and Schmitt, R. (1976) Structure of plain and complex flagellar hooks of Pseudomonas rhodos. J Bacteriol 125: 679-688.
29. Salmon, E.D., and DeRosier, D.J. (1981) A surveying optical diffractometer. J Microsc 123: 239-247.
30. Samatey, F.A., Imada, K., Nagashima, S., Vonderviszt, F., Yamamoto, M., and Namba, K. (2001) Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature 410: 331-337.
31. Scharf, B., Schuster-Wolff-Buhring, H., Rachel, R., and Schmitt, R. (2001) Mutational analysis of the Rhizobium lupini H13-3 and Sinorhizobium meliloti flagetlin genes: importance of flagellin A for flagellar filament structure and transcriptional regulation. J Bacteriol 183: 5334-5342.
32. Schmitt, R., Raska, I., and Mayer, F. (1972) [Fine structure and biochemistry of complex flagella and their flagellum hook in Pseudomonas rhodos]. Hoppe Seylers Z Physiol Chem 353: 1564-1565.
33. Schmitt, R., Bamberger, I., Acker, G., and Mayer, F. (1974a) Feinstrukturanalyse der komplexen geisseln von Rhizobium lupini H13-3. Arch Microbiol 100: 145-162.
34. Schmitt, R., Raska, I., and Mayer, F. (1974b) Plain and complex flagella of Pseudomonas rhodos: Analysis of fine structure and composition. J Bacteriol 117: 844-857.
35. Sourjik, V., and Schmitt, R. (1996) Different roles of CheY1 and CheY2 in the chemotaxis of Rhizobium meliloti. Mol Microbiol 22: 427-436.
36. Sourjik, V., Sterr, W., Platzer, J., Bos, I., Haslbeck, M., and Schmitt, R. (1998) Mapping of 41 chemotaxis, flagellar and motility genes to a single region of the Sinorhizobium meliloti chromosome. Gene 223: 283-290.
37. Sourjik, V., Muschler, P., Scharf, B., and Schmitt, R. (2000) VisN and VisR are global regulators of chemotaxis, flagellar, and motility genes in Sinorhizobium (Rhizobium) meliloti. J Bacteriol 182: 782-788.
38. Toyoshima, C., and Unwin, N. (1988) Contrast transfer for frozen-hydrated specimens: determination from pairs of defocused images. Ultramicroscopy 25: 279-292.
39. Trachtenberg, S. (1993) Fast-freezing devices for cryo-electron-microscopy. Micron 24: 1-12.
40. Trachtenberg, S. (1998) A fast-freezing device with a retractable environmental chamber, suitable for kinetic cryo-electron microscopy studies. J Struct Biol 123: 45-55.
41. Trachtenberg, S., and DeRosier, D.J. (1987) Three-dimensional structure of the frozen-hydrated flagellar filament. The left-handed filament of Salmonella typhimunum. J Mol Biol 197: 581-601.
42. Trachtenberg, S., and DeRosier, D.J. (1988) Three-dimensional reconstruction of the flagellar filament of Caulobacter crescentus. A flagellin lacking the outer domain and its amino acid sequence lacking an internal sequence. J Mol Biol 202: 787-808.
43. Trachtenberg, S., and DeRosier, D.J. (1991) A molecular switch: subunit rotations involved in the right-handed to left-handed transitions of Salmonella typhimurium flagellar filaments. J Mol Biol 220: 67-77.
44. Trachtenberg, S., and DeRosier, D.J. (1992) Conformational switching in the flageltar filament of Salmonella typhimurium. J Mol Biol 226: 447-454.
45. Trachtenberg, S., and Hammel, I. (1992) The rigidity of bacterial flagellar filaments and its relation to filament polymorphism. J Struct Biol 109: 18-27.
46. Trachtenberg, S., DeRosier, D.J., Aizawa, S.-I., and Macnab, R.M. (1986) Pairwise perturbation of flagellin subunits. The structural basis for the differences between plain and complex bacterial flagellar filaments. J Mol Biol 190: 569-576.
47. Trachtenberg, S., DeRosier, D.J., and Macnab, R.M. (1987) Three-dimensional structure of the complex flageltar filament of Rhizobium lupini and its relation to the structure of the plain filament. J Mol Biol 195: 603-620.
48. Trachtenberg, S., DeRosier, D.J., Zemlin, F., and Beckman, E. (1998) Non-helical perturbations of the flagellar filament: Salmonella typhimurium SJW117 at 9.6 A resolution. J Mol Biol 276: 759-773.
49. Trachtenberg, S., Fishelov, D., and Ben Artzi, M. (2003) Bacterial flagellar microhydrodynamics: laminar flow over complex flagellar filaments, analog Archimedean screws and cylinders and its pertubations. Biophys J (in press).
50. Yoshioka, K., Aizawa, S.-I., and Yamaguchi, S. (1995) Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin. J Bacteriol 177: 1090-1093.