On-line monitoring of cell growth and cytotoxicity using electric cell-substrate impedance sensing (ECIS)

Biotechnology Progress

Volumn 19, Issue 3, 2003, Pages 1000-1005

  Xiao, Caide ^a   Luong, John H T ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOTOXICITY;

CHLORINE COMPOUNDS; ELECTRIC IMPEDANCE; MATHEMATICAL MODELS; ONLINE SYSTEMS;

ELECTRIC BATTERIES;

ANIMALS; BIOSENSING TECHNIQUES; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIVISION; CELLS, CULTURED; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTRIC IMPEDANCE; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FIBROBLASTS; LETHAL DOSE 50; LUNG; MERCURIC CHLORIDE; MODELS, BIOLOGICAL; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TOXICITY TESTS; TRINITROBENZENES;

EID: 0038119635     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp025733x     Document Type: Article

References (16)

1. Heaysman, J. M. E.; Pergum, S. M. In Cell Behavior; Bellairs, R., Curtis, A., Dunn, G., Eds: Cambridge University Press: London, 1982; pp 49-76.
2. Giaever, I.; Keese, C. R. A Morphological Biosensor for Mammalian Cells. Nature 1993, 366, 591-592.
3. Borkholder, D. A.; DeBusschere, B. D.; Kovacs, G. T. A. An Approach to the Classification of Unknown Biological Agents with Cell Based Sensors. Technical Digest of the 1998 Solid-State Sensor and Actuator Workshop, Hilton Head Island, SC, June 2-11, 1998.
4. Wegener, J.; Keese, C. R.; Giaever, I. Electric Cell-Substrate Impedance Sensing (ECIS) as a Noninvasive Means to Monitor the Kinetics of Cell Spreading to Artificial Surfaces. Exp. Cell Res. 2000, 259, 158-166
5. Giaever, I.; Keese, C. R. Toxic? Cells Can Tell. CHEMTECH 1992, 116-125.
6. Keese, C. R.; Karra, N.; Dillon, B.; Goldberg, A. M.; Giaever, I. Cell-subtratum Interactions as a Predictor of Cytotoxicity. In Vitro Mol. Toxicol. 1998, 11(2), 183-192.
7. Keese, C. R.; Giaever, I. A Biosensor that Monitors Cell Morphology with Electrical Fields. IEEE Eng. Med. Biol. 1994, 13(3), 402-408.
8. Luong, J. H. T.; Habibi-Razaei; M.; Meghrous, J.; Xiao, C. D.; Male, K. B.; Kamen, A. Monitoring Motility, Spreading, and Mortality of Adherent Insect Cells Using an Impedance Sensor. Anal. Chem. 2001, 73, 1844-1848.
9. Xiao, C. D.; Lachance, B.; Sunahara, G.; Luong, J. H. T. An In-depth Analysis of Electric Cell-Substrate Impedance Sensing to Study the Attachment and Spreading of Mammalian Cells. Anal. Chem. 2002, 74, 1333-1339.
10. Xiao, C. D.; Lachance, B.; Sunahara, G.; Luong, J. H. T. Assessment of Cytotoxicity using Electric Cell-Substrate Impedance Sensing: Concentration and Time Response Function Approach. Anal. Chem. 2002, 74, 5748-5753.
11. http://www.atsdr.cdc.gov/tfacts46.html, ToxFAQs for Mercury, CAS no. 7439-97-6.
12. U.S.E.P.A. Support Document for 1,3,5-Trinitrobenzene (TNB), CAS no. 99-35-4. National Center for Environmental Assessment and National Exposure Research Laboratory: Cincinnati, OH, 1997 (http://www.epa.gov/iris/supdocs/tnb-sup.pdf).
13. Dorsey, A.; Llaods, F. ATSDR (Agency for Toxic Substances and Disease Registry); Toxicological Profiles: 1,3-Dinitrobenzene and 1,3,5-Trinitrobenzene. CRC Press: London, 1999.
14. Freshney, R. Culture of Animal Cells: A Manual of Basic Techniques, A. R. Liss: New York, 1987.
15. Borenfreund, E.; Puerner, J. A. A Simple Quantitative Procedure using Monolayer Cultures for Cytotoxicity Assays (HTD/NR-90). J. Tissue Cult. Methods 1984, 9, 7-9.
16. ATCC, the Global Bioresource Center; http://www.atcc.org.