Characterization of single-tryptophan mutants of histidine-containing phosphocarrier protein: Evidence for local rearrangements during folding from high concentrations of denaturant

Biochemistry

Volumn 42, Issue 17, 2003, Pages 4883-4895

  Azuaga, Ana I ^a   Canet, Denis ^b,f   Smeenk, Gerard ^c   Berends, Roel ^c   Titgemeijer, Fritz ^d   Duurkens, Ria ^c   Mateo, Pedro L ^e   Scheek, Ruud M ^c   Robillard, George T ^c   Dobson, Christopher M ^b,g   Van Nuland, Nico A J ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

^d UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^e UNIVERSITY OF GRANADA   (Spain)

^f Geneva Proteomics Inc   (Switzerland)

^g UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ANISOTROPY; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE; QUENCHING;

FLUORESCENCE ANISOTROPY;

PROTEINS;

CARRIER PROTEIN; GLOBULAR PROTEIN; MUTANT PROTEIN; PHENYLALANINE; PHOSPHOPROTEIN; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; ANISOTROPY; ARTICLE; CIRCULAR DICHROISM; DIFFERENTIAL SCANNING CALORIMETRY; FLUORESCENCE; HYDROPHOBICITY; KINETICS; MODEL; MOLECULAR PROBE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; FLUORESCENT DYES; GUANIDINE; HISTIDINE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; TRYPTOPHAN;

EID: 0038031767     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi027182p     Document Type: Article

References (44)

1. Radford, S. E., and Dobson, C. M. (1999) Cell 97, 291-298.
2. Pain, R. H. (1999) Mechanisms in Protein Folding, 2nd ed., Oxford University Press, Oxford.
3. Myers, J. K., and Oas, T. G. (2002) Annu. Rev. Biochem. 71, 783-815.
4. Jackson, S. E. (1998) Folding Des. 3, R81-R91.
5. Gunasekaran, K., Eyles, S. J., Hagler, A. T., and Gierasch, L. M. (2001) Curr. Opin. Struct. Biol. 11, 83-93.
6. Fersht, A. R. (1999) Structure and Mechanism in Protein Science, Freeman, New York.
7. Chiti, F., Taddei, N., White, P. M., Bucciantini, M., Magherini, F., Stefani, M., and Dobson, C. M. (1999) Nat. Struct. Biol. 11, 1005-1009.
8. Plaxco, K. W., Simons, K. T., and Baker, D. (1998) J. Mol. Biol. 277, 985-994.
9. Postma, P. W., Lengeler, J. W., and Jacobson, G. R. (1993) Microbiol. Rev. 57, 543-594.
10. Van Nuland, N. A. J., Hangyi, I. W., Van Schaik, R. C., Berendsen, H. J. C., Van Gunsteren, W. F., Scheek, R. M., and Robillard, G. T. (1994) J. Mol. Biol. 237, 544-559.
11. Jia, Z., Quail, J. W., Waygood, E. B., and Delbaere, L. T. J. (1993) J. Biol. Chem. 268, 22490-22501.
12. Swindells, M. B., Orengo, C. A., Jones, D. T., Pearl, L. H., and Thornton, J. M. (1993) Nature 362, 299.
13. Van Nuland, N. A. J., Meijberg, W., Warner, J., Forge, V., Scheek R. M., Robillard, G. T., and Dobson, C. M. (1998) Biochemistry 37, 622-637.
14. Landt, O., Grunert, H. P., and Hahn, U. (1990) Gene 96, 125-128.
15. Van Nuland, N. A. J., Van Dijk, A. A., Dijksrtra, K., Van Hoesel, F. H. J., Scheek R. M., and Robillard, G. T. (1992) Eur. J. Biochem. 203, 483-491.
16. Robillard, G. T., and Blaauw, M. (1987) Biochemistry 26, 5796-5803.
17. Gill, S. C., and Von Hippel, P. H. (1989) Anal. Biochem. 182, 319-326.
18. Pace, C. N. (1986) Methods Enzymol. 131, 266-280.
19. Visser, A. J. W. G., Van Engelen, J., Visser, N. V., Van Hoek, A., Hilhorst, R., and Freedman, R. B. (1994) Biochim. Biophys. Acta 1204, 225-234.
20. Hwang, T.-L, and Shaka, A. J. (1995) J. Magn. Reson. A112, 275-279.
21. Privalov, P. L., and Plotnikov, S. A. (1989) Thermochim. Acta 139, 257-277.
22. López-Mayorga, O., and Freire, E. (1987) Biophys. Chem. 27, 87-96.
23. Privalov, P. L., and Potekhin, S. V. (1986) Methods Enzymol. 114, 4-51.
24. Vigera, A. R., Martinez, J. C., Filimonov, V. V., Mateo, P. L., and Serrano, L. (1994) Biochemistry 33, 2142-2150.
25. Canet, D., Doering, K., Dobson, C. M., and Dupont, Y. (2001) Biophys. J. 80, 1996-2003.
26. Otto, M. R., Lillo, M. P., and Beechem, J. M. (1994) Biophys. J. 67, 2511-2521.
27. Live, D. H., Davis, D. G., Agosta, W. C., and Cowburn, D. (1984) J. Am. Chem. Soc. 106, 1939-1941.
28. Redfield, A. G., and Kunz, S. D. (1975) J. Magn. Reson. 19, 250-254.
29. Lolkema, J. S., Ten Hoeve-Duurkens, R. H., and Robillard, G. T. (1993) J. Biol. Chem. 268, 17844-17849.
30. Van Nuland, N. A. J., Kroon, G. J. A., Dijkstra, K., Wolters, G. K., Scheek, R. M., and Robillard, G. T. (1993) FEBS Lett. 315, 11-15.
31. Eftink, M. R. (1991) Methods Biochem. Anal. 35, 127-207.
32. Ross, J. B. A., Wyssbrod, H. R., Porter, A. R., Schwartz, G. P., Michaels, C. A., and Laws, W. R. (1992) Biochemistry 31, 1585-1594.
33. Lakowicz, J. R. (1983) Principles of fluorescence spectroscopy, Plenum Press, New York.
34. Van Nuland, N. A. J., Boelens, R., Scheek, R. M., and Robillard, G. T. (1995) J. Mol. Biol. 246, 180-193.
35. Canet, D., Lyon, C., Robillard, G. T., Scheek, R. M., Dobson, C. M., Hore, P. J., and Van Nuland, N. A. J. (2002) (submitted for publication).
36. Privalov, P. L., and Gill, S. J. (1988) Adv. Protein Chem. 39, 191-234.
37. Alexander, P., Fahnestock, S., Lee, T., Orban, J., and Bryan, P. (1992) Biochemistry 31, 3597-3603.
38. Eftink, M. R. (2000) Methods Enzymol. 323, 459-473.
39. Henry, E. R. (1997) Biophys. J. 72, 652-673.
40. Itzhaki, L. S., Evans, P. A., Dobson, C. M., and Radford, S. E. (1994) Biochemistry 33, 5212-5220.
41. Villegas, V., Martinez, J. C., Aviles, F. X., and Serrano, L. (1998) J. Mol. Biol. 283, 1027-1036.
42. Chiti, F., Taddei, N., Giannoni, E., Van Nuland, N. A. J., Ramponi, G., and Dobson, C. M. (1999) J. Biol. Chem. 274, 20151-20158.
43. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
44. Bundi, A., and Wüthrich, K. (1979) Biopolymers 18, 285-297.