Drug-induced haploinsufficiency of fission yeast provides a powerful tool for identification of drug targets

Journal of Microbiology and Biotechnology

Volumn 13, Issue 2, 2003, Pages 317-320

  Park, Jo Young ^d   Jang, Young Joo ^d   You, Seog Jong ^a   Kil, Young Sook ^d   Kang, Eun Jung ^d   Ahn, Jee Hee ^d   Ryoo, Young Kwon ^d   Lee, Min Youn ^d   Park, Sun Yong ^b   Lee, Hyun Jee ^b   Kim, Jee Youn ^b   Kim, Sun Hye ^b   Yang, Wan Soo ^b   Song, Kyung Bin ^b   Park, Hee Moon ^b   Chung, Young Jin ^b   Kim, Hyong Bai ^c   Hoe, Kwang Lae ^d   Chung, Kyung Sook ^d   Kim, Dong Uk ^d   Yoo, Hyang Sook ^d   Won, Misun ^d   more..

^a Bioneer Corporation   (South Korea)

^b Chungnam National University   (South Korea)

^c Korea University   (South Korea)

^d Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Drug target; Haploinsufficiency; PCR based targeted mutagenesis; Schizosaccharomyces pombe

Indexed keywords

MICROTUBULE PROTEIN; TIABENDAZOLE;

ARTICLE; CONTROLLED STUDY; DRUG RESEARCH; DRUG SENSITIVITY; DRUG TARGETING; FUNGUS MUTANT; GENE DELETION; GENE MUTATION; GENE TARGETING; GENETIC ANALYSIS; GENETIC CODE; HAPLOIDY; HETEROZYGOSITY; INHIBITION KINETICS; MICROTUBULE ASSEMBLY; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SCHIZOSACCHAROMYCES POMBE;

FUNGI; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE;

EID: 0038025773     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Adachi, Y., T. Toda, O. Niwa, and M. Yanagida. 1986. Differential expressions of essential and nonessential alpha-tubulin genes in Schizosaccharomyces pombe. Mol. Cell. Biol. 6: 2168-2178.
2. Bahler, J., J. Q. Wu, M. S. Longtine, N. G. Shah, A. McKenzie, A. B. Steever, A. Wach, P. Philippsen, and J. R. Pringle. 1998. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951.
3. Fisher, E. and P. Scambler. 1994. Human haploinsufficiency - one for sorrow, two for joy. Nat. Genet. 7: 5-75.
4. Giaever, G., D. D. Shoemaker, T. W. Jones, H. Liang, E. A. Winzeler, A. Astromoff, and R. W. Davis. 1999. Genomic profiling of drug sensitivities via induced haploinsufficiency. Nat. Genet. 21: 278-283.
5. Kwon, H. J., M. Kim, and S. Y. Kim. 2002. New yeast cell-based assay system for screening histone deacetylase I complex disruptor. J. Microbiol. Biotechnol. 12: 286-291.
6. Lindsley, D. L., L. Sandler, B. S. Baker, A. T. Carpenter, R. E. Denell, J. C. Hall, P. A. Jacobs, G. L. Miklos, B. K. Davis, R. C. Gethmann, R. W. Hardy, A. H. Steven, M. Miller, H. Nozawa, D. M. Parry, M. Gould-Somero, and M. Gould-Somero. 1972. Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics 71: 157-184.
7. Longtine, M. S., A. McKenzie, D. J. Demarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen, and J. R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in S. cerevisiae. Yeast 14: 953-961.
8. Moreno, S., A. Klar, and P. Nurse. 1991. Molecular genetic analysis of fission yeast Schizosccharomyces pombe. Methods Enzymol. 194: 795-823.
9. Wach, A., A. Brachat, R. Pohlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
10. Winzeler, E. A., D. D. Shoemaker, A. Astromoff, H. Liang, K. Anderson, B. Andre, R. Bangham, R. Benito, J. D. Boeke, H. Bussey, A. M. Chu, C. Connelly, K. Davis, F. Dietrich, S. W. Dow, M. El Bakkoury, F. Foury, S. H. Friend, E. Gentalen, G. Giaever, J. H. Hegemann, T. Jones, M. Laub, H. Liao, and R. W. Davis. 1999. Functional characterization of S. cerevisiae genome by gene deletion and parallel analysis. Science 285: 901-906.
11. Won, M., Y.-J. Jang, K.-W. Hoe, J.-Y. Park, K.-S. Chung, D.-U. Kim, N.-K. Sun, S.-A. Kim, K. B. Song, and H-S. Yoo. 2002. Rkp1/CPC2, a RACK1 homolog, interacts with Pkc1 to regulate PKC-mediated signaling in Schizosacchromyces pombe. J. Microbiol. Biotechnol. 12: 592-597.
12. Wood, V., R. Gwilliam, M. A. Rajandream, M. Lyne, R. Lyne, A. Stewart, J. Sgouros, N. Peat, J. Hayles, S. Baker, D. Basham, S. Bowman, K. Brooks, D. Brown, S. Brown, T. Chillingworth, C. Churcher, M. Collins, R. Connor, A Cronin, P. Davis, T. Feltwell, A. Fraser, S. Gentles, A. Goble, N. Hamlin, D. Harris, J. Hidalgo, G. Hodgson, S. Holroyd, T. Hornsby, S. Howarth, E. J. Huckle, S. Hunt, K. Jagels, K. James, L. Jones, M. Jones, S. Leather, S. McDonald, J. McLean, P. Mooney, S. Moule, K. Mungall, L. Murphy, D. Niblett, C. Odell, K. Oliver, S. O'Neil, D. Pearson, M. A. Quail, E. Rabbinowitsch, K. Rutherford, S. Rutter, D. Saunders, K. Seeger, S. Sharp, J. Skelton, M. Simmonds, R. Squares, S. Squares, K. Stevens, K. Taylor, R. G. Taylor, A. Tivey, S. Walsh, T. Warren, S. Whitehead, J. Woodward, G. Volckaert, R. Aert, J. Robben, B. Grymonprez, I. Weltjens, E. Vanstreels, M. Rieger, M. Schafer, S. Muller-Auer, C. Gabel, M. Fuchs, C. Fritzc, E. Holzer, D. Moestl, H. Hilbert, K. Borzym, I. Langer, A. Beck, H. Lehrach, R. Reinhardt, T. M. Pohl, P. Eger, W. Zimmermann, H. Wedler, R. Wambutt, B. Purnelle, A. Goffeau, E. Cadieu, S. Dreano, S. Gloux, et al. 2002. The genome sequence of Schizosaccharomyces pombe. Nature 415: 871-880.