A model with original conditions and materials to test potential inhibitors of cyclooxygenase-2 versus cyclooxygenase-1 in isolated human blood cells

Methods and Findings in Experimental and Clinical Pharmacology

Volumn 25, Issue 3, 2003, Pages 175-181

  Siomboing, X ^a   D'Hondt, O ^a   Luyckx, M ^a   Gressier, B ^a   Dine, T ^a   Brunet, C ^a  

^a UNIV LILLE   (France)

Author keywords

Arachidonic acid; Cyclooxygenase; Inhibition; Lymphocytes; Monocytes

Indexed keywords

ACETYLSALICYLIC ACID; ARACHIDONIC ACID; CALCIUM IONOPHORE; CYCLOOXYGENASE 1; CYCLOOXYGENASE 1 INHIBITOR; CYCLOOXYGENASE 2; CYCLOOXYGENASE 2 INHIBITOR; IBUPROFEN; ISOENZYME; LIPOPOLYSACCHARIDE; NIMESULIDE; THROMBOXANE B2;

ARTICLE; DRUG MECHANISM; DRUG SPECIFICITY; ENZYME ACTIVATION; ENZYME INHIBITION; ENZYME SYNTHESIS; HUMAN; HUMAN CELL; IC 50; INFLAMMATION; LYMPHOCYTE; MONOCYTE; RADIOIMMUNOASSAY;

ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; ARACHIDONIC ACID; BLOTTING, WESTERN; CYCLOOXYGENASE 1; CYCLOOXYGENASE 2; HUMANS; ISOENZYMES; LIPOPOLYSACCHARIDES; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; PROSTAGLANDIN-ENDOPEROXIDE SYNTHASES;

EID: 0037986445     PISSN: 03790355     EISSN: None     Source Type: Journal    
DOI: 10.1358/mf.2003.25.3.769636     Document Type: Article

References (37)

1. Copeland R.A., Williams J.M., Giannaras J. et al. Mechanism of selective inhibition of the inductible of prostaglandin G/H synthase. Proc Natl Acad Sci USA 1994, 91: 11202-6.
2. De Witt D.L., Prostaglandin endoperoxide synthase: Regulation of enzyme expression. Biochim Biophys Acta 1991, 1083: 121-34.
3. Xie W., Chapman J.G., Robertson D.L., Erikson R.L., Simmons D.L. Expression or a mitogen responsive gene encoding prostaglandin synthase is regulated mRNA splicing. Proc Natl Acad Sci USA 1991, 88: 2692-6.
4. Vane J.R., Mitchell J.A., Appleton I., Inducible isoforms of cyclo-oxygenase and synthase in inflammation. Proc Natl Acad Sci USA 1994, 91: 2046-50.
5. Frolich J.C., A classification of NSAIDs according to the relative inhibition of cyclooxygenase enzyme. Trends Pharmacol Sci 1997, 18:30-4.
6. Habib A., Creminon C., Frobert Y., Grassi J., Pradelles P., Maclouf J. Demonstration of an inducible cyclooxygenase in human endothelial cells using antibodies raised against the carboxyl-terminal region of the cyclooxygenase-2. J Biol Chem 1993, 268: 23448-54.
7. Patrigani P., Panara M.R., Greco A. et al. Biochemical and pharmacological characterization of the cyclooxygenase activity of human blood prostaglandin endoperoxide synthases. J Pharmacol Exp Ther 1994, 271: 1705-12.
8. Wilborn J., De Witt D.L., Peters-Goldin M., Expression and role of cyclooxygenase isoforms in alveolar and peritoneal macrophages. Am J Physiol 1995, 268: 294-301.
9. Grossman C.J., Wiseman J., Lucas, F.S., Trevethick, M.A., Birch P.J. Inhibition of constitutive and cyclooxygenase activity in human platelets and mononuclear cells by NSAIDs and Cox2 inhibitors. Inflamm Res 1995, 44: 256-7.
10. Fletcher, B.S., Kubuju, D.A., Perrin, D.M., Herscham, H.R. Structure of the mitogen-inducible TIS 10 gene and demonstration that the TIS 10-encoded protein is a functional prostaglandin G/H synthase. J Biol Chem 1992, 267: 4334-48.
11. Kraemer, S.A., Meade, S.A., De Witt, D.L. Prostaglandin endoperoxide synthase gene structure: Identification of the transcriptional start site and 5′-flanfing regularity sequences. Arch Biochem, Biophys 1992, 293: 391-400.
12. Meade, E.A., Smith, W.L., De Witt, D.L. Differential inhibition of prostaglandin endoperoxide synthase (cyclooxygenase) isozymes by aspirin and other non-steroidal anti-inflammatory drugs. J Biol Chem 1993, 268: 6610-4.
13. Mitchell, J.A., Akarasereenont, P., Thiemermann, C., Flower, R.J., Vane J.R. Selectivity of nonsteroidal antiflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase. Proc Natl Acad Sci USA 1993, 90: 11693-7.
14. Hla, T, Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 1992, 89: 7384-8.
15. Reitz, D.B., Isakson, P.C. Cyclooxygenase-2 inhibitors. Current Pharm Design 1995, 1: 211-20.
16. Reitz D.B., Seibert, K. Selective cyclooxygenase inhibitors. Annual Report Med Chem 1995, 30: 179-86.
17. Kuehl F.A., Egan R.W., Prostaglandins, arachidonic acid and inflammation. Science 1980, 210: 978-83.
18. 2 synthase-1. Nature 1994, 367: 243-9.
19. Cabanis, A., Gressier, B., Lebègue, S. et al. A rapid density gradient technique for separating polyinorphonuclear granulocytes. AMPIS 1994, 102: 119-21.
20. Brideau, C., Kargman, S., Liu, S. et al. A human blood assay for clinical evaluation of biochemical efficacy of cyclooxygenase inhibitors. Inflamm Res 1996, 45: 68-74.
21. Vane, J.R., Botting, R.M. Antiinflammatory drugs and their mechanism of action. Inflamm Res 1998, 47(2): S78-87.
22. Salmon, J.A., Flower, R.J. Extraction and thin-layer chomatography of arachidonic acid metabolites. Methods Enzymol 1986, 86: 477-93.
23. 2 synthase (cyclooxygenase) in human monocytes. J Biol Chem 1990, 265: 16737-40.
24. Tomlinson, A., Appleton, I., Moore, A.R. et al. Cyclo-oxygenase and nitric oxide synthase isoforms in rat carrageenin-induced pleurisy. Br J Pharmacol 1994, 113: 693-8.
25. Barry O.P., Kazanietz, M.G., Pratico, D., Fitzgerald G.A. Arachidonic acid in platelet microparticules up-regulates cyclooxy-gelutse-2 dependent prostaglandin formation via a protein kinase C/mitogen-activated protein kinase-dependent pathway. J Biol Chem 1999, 274: 7545-56.
26. Young, J.M., Panah, C., Satchawatcharaphong, Cheung P.S., Human whole blood assays for inhibition of prostaglandin G/H synthases-1 and -2 using A23187 and lipopolysaccharide stimulation of thromboxane B2 production. Inflamm Res 1996, 45: 246-53.
27. O'Neill, G.P., Mancini, J.A., Kargman, S., Yergey, J., Kwan, M.Y., Falgueyret J.P. Overexpression of human prostaglandin G/H synthase-1 and -2 recombinant vaccinia virus: Inhibition by nonsteroidal anti-inflammatory drugs and biosynthesis of 15-hydroxye-icosatetraenoic acid. Mol Pharmacol 1994, 45: 245-54.
28. Riendeau, D., Percival M.D., Boyce S., Brideau C., Charleson S., Cromlish W., Biochemical and pharmacological profile of a tetrasubstitued furanone as a highly selective COX-2 inhibiton. Br J Pharmacol 1997, 121: 105-17.
29. Tavares, I.A., Bishai, P.M., Bennett, A. Activity of niniesulide on constitutive and inductible cyclooxygenases. Arzneimittelforschung 1995, 45: 1093-5.
30. Famaey, J.P. In vitro and in vivo pharmacological evidence of selective cyclooxygenase-2 inhibition by nimesulide: An overview. Inflamm Res 1997, 46: 437-46.
31. Greyn J., Lauwers, M.H., Vanlersberghe, C., Camu, F. COX-1 and COX-2 inhibition: Current status and future perspective. Acta Anaesth Belg 1998, 49: 175-83.
32. Pairet, M., Van Ryn, J. Experimental models used to investigate the differential inhibition of cyclooxygenase-1 and cycloxygenase-2 by non-steroidal anti-inflammatory drugs. Inflamm Res 1998, 47: 93-101.
33. Barnett, J., Chow, J., Ives, D., Chiou, M., MacKenzie, R., Osen E. Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus system. Biochim Biophys Acta 1994, 1209: 130-9.
34. Laneuville, O., Breuer, D.K., DeWitt, D.L., Hla, T., Funf, C.D., Smith, W.L. Differential inhibition of human prostaglandin endoperoxide H synthases-1 and -2 by nonsteroidal Antiinflammatory drugs. J Pharmacol Exp Therap 1994, 271: 927-34.
35. Katori, M., Majima, M. CyclooAygenase-2: Its rich diversity of roles and possible application of its selective inhibitors. Inflamm Res 2000, 49: 367-92.
36. , Cullen, L., Kelly, L., Connor, S.O., Fitzgerald, D.J. Selective cyclooxygenase-2 inhibition by nimesulide in man. J Pharmacol Exp Therap 1998, 28: 578-82.
37. Kawai, S. Cyclooxygenase selectivity and the risk of gastro-intestinal complications of various non-steroidal anti-inflammatory drugs: A clinical consideration. Inflamm Res 1998, 2: S102-6.