A protocol for isolation and biochemical characterization of stigmoid bodies from rat brain

Journal of Neuroscience Methods

Volumn 125, Issue 1-2, 2003, Pages 27-32

  Torre, Enrique R ^a   Coleman, Sarah ^a   Yi, Hong ^a   Gutekunst, Claire Anne ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Huntingtin associated protein 1 (HAP1); LR11; Nucleolus like bodies; SorLA

Indexed keywords

BRAIN ANTIBODY; BRAIN PROTEIN; HUNTINGTIN; ISOPROTEIN; LIPOPROTEIN RECEPTOR; SUCROSE;

ANIMAL TISSUE; ARTICLE; BIOCHEMISTRY; BRAIN NERVE CELL; BRAIN TISSUE; CELL ULTRASTRUCTURE; CENTRIFUGATION; CLINICAL PROTOCOL; ELECTRON MICROSCOPY; LIGHT; MAGNETIC SEPARATION; NEUROBIOLOGY; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; RAT; SAMPLING; SUCROSE GRADIENT; TECHNIQUE;

EID: 0037902128     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00026-8     Document Type: Article

References (20)

1. Chan E., Nassir J., Gutekunst C.A., Coleman S., Maclean A., Mass A., Metzler M., Gentsenstein M., Ross C., Nagy A., Hayden M. Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Human Molecular Genetics. 11:2002;945-959.
2. Fechner J. Nucleolus-like bodies in the pineal gland of the Djungarian hamster (Phodopus sungorus). Cell Tissue Research. 243:1986;441-443.
3. Gutekunst C., Li S., Yi H., Ferrante R., Li X., Hersch S. The cellular and subcellular localization of huntingtin-associated protein 1 (HAP1): comparison with huntingtin in rat and human. Journal of Neuroscience. 18:1998;7674-7686.
4. Gutekunst C, Torre E, Sheng Z, Yi H, Coleman S, Riedel IB, Bujo H. Stigmoid bodies contain type I receptor proteins SorLA/LR11 and sortilin: new perspectives on their function. Journal of Histochemistry and Cytochemistry 2003;51:in press.
5. Henley J.R., McNiven M.A. Association of a dynamin-like protein with the Golgi apparatus in mammalian cells. Journal of Cell Biology. 133:1996;761-775.
6. Katoh Y., Shimizu N. Identity of holmes positive bodies with electron microscopically demonstrate nucleolus-like bodies in neuronal cytoplasm. Stain Technology. 57:1982;83-89.
7. Kedersha N.L., Miquel M.C., Bittner D., Rome L.H. Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes. Journal of Cell Biology. 110:1990;895-901.
8. Kishi K. Fine structural and cytochemical observations on cytoplasmic nucleolus like bodies in nerve cells of rat medulla oblongata. Zeitschrift Zellforschung. 132:1972;523-532.
9. Larriva-Sahd J., Gorski R. Ultrastructual characterization of the central component of the medial preoptic nucleus. Experimental Neurology. 98:1987;370-387.
10. Leranth C., Shanabrough M., Naftolin F. Estrogen induces ultrastructural changes in progesterone receptor-containing GABA neurons of the primate hypothalamus. Neuroendocrinology. 54:1991;571-579.
11. Li X., Li S., Sharp A., Nucifora F., Schilling G., Lanahan A., Worley P., Snyder S., Ross C. A huntingtin-associated protein enriched in brain with implications for pathology. Nature. 378:1995;398-402.
12. Luers G.H., Hartig R., Mohr H., Hausmann M., Fahimi H.D., Cremer C., Volkl A. Immuno-isolation of highly purified peroxisomes using magnetic beads and continuous immunomagnetic sorting. Electrophoresis. 19:1998;1205-1210.
13. Navarro A., Rey C.G., Tolivia J., Alvarez-Uria M. Ultrastructural and quantitative study of atypical age-related bodies in the hamster brain. Mechanisms of Ageing and Development. 90:1996;75-90.
14. Page K., Potter L., Aronni S., Everitt B., Dunnett S. The expression of Huntingtin-associated protein (HAP1) mRNA in developing, adult and ageing rat CNS: implications for Huntington's disease neuropathology. European Journal of Neuroscience. 10:1998;1835-1845.
15. Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates. 2nd ed:1986;Academic Press, Harcourt Brace Jovanovich, San Diego.
16. Santolaya R. Nucleolus-like bodies in the neuronal cytoplasm of the mouse arcuate nucleus. Zeitschrift Zellforschung. 146:1973;319-328.
17. Shinoda K., Nagano M., Osawa Y. An aromatase-associated cytoplasmic inclusion, the 'Stigmoid Body', in the rat brain: II. Ultrastructure (with a review of its history and nomenclature). The Journal of Comparative Neurology. 329:1993;1-19.
18. Taira K., Bujo H., Hirayama S., Yamazaki H., Kanaki T., Takahashi K., Ishii I., Miida T., Schneider W.J., Saito Y. LR11, a mosaic LDL receptor family member, mediates the uptake of ApoE-rich lipoproteins in vitro. Arterioscler Thrombosis Vascular Biology. 21:2001;1501-1506.
19. Takamori S., Riedel D., Jahn R. Immunoisolation of GABA-specific synaptic vesicles defines a functionally distinct subset of synaptic vesicles. Journal of Neuroscience. 20:2000;4904-4911.
20. Tasso F., Rua S. Ultrastructural observations on the hypothalamo-posthypophysial complex of the brattleboro rat. Cell and Tissue Research. 191:1978;267-286.