Altered myocardial Gs protein and adenylyl cyclase signaling in rats exposed to chronic hypoxia and normoxic recovery

Journal of Applied Physiology

Volumn 94, Issue 6, 2003, Pages 2423-2432

  Hrbasová, Markéta ^a   Novotny, Jiri ^a,b   Hejnová, Lucie ^a,b   Kolář, František ^b,c   Neckář, Jan ^b,c   Svoboda, Petr ^a,b  

^a CHARLES UNIVERSITY   (Czech Republic)

^b INSTITUTE OF PHYSIOLOGY   (Czech Republic)

^c Ctr for Exp Cardiovasc Research   (Czech Republic)

Author keywords

Beta adrenoceptors; G proteins; Rat myocardium

Indexed keywords

4 (3 TERT BUTYLAMINO 2 HYDROXYPROPOXY) 2 BENZIMIDAZOLONE; ADENYLATE CYCLASE; BETA ADRENERGIC RECEPTOR; FLUORIDE; FORSKOLIN; GUANOSINE TRIPHOSPHATE; ISOPRENALINE; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN; CARDIOTONIC AGENT; ISOPROTEIN;

ADAPTATION; ALTITUDE DISEASE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; DENSITOMETRY; ENZYME ACTIVITY; HEART CONTRACTION; HEART LEFT VENTRICLE FUNCTION; HEART LEFT VENTRICLE HYPERTROPHY; HEART MUSCLE ISCHEMIA; HEART RIGHT VENTRICLE FUNCTION; HEART RIGHT VENTRICLE PRESSURE; HEART WEIGHT; IMMUNOBLOTTING; INOTROPISM; MALE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RAT; ANIMAL; ANOXIA; ATMOSPHERIC PRESSURE; CHRONIC DISEASE; HEART MUSCLE; METABOLISM; ORGAN SIZE; PATHOLOGY; SIGNAL TRANSDUCTION; WISTAR RAT;

ADENYLATE CYCLASE; ANIMALS; ANOXIA; ATMOSPHERIC PRESSURE; BODY WEIGHT; CARDIOTONIC AGENTS; CHRONIC DISEASE; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; ISOPROTERENOL; MALE; MYOCARDIAL CONTRACTION; MYOCARDIUM; ORGAN SIZE; PROTEIN ISOFORMS; RATS; RATS, WISTAR; RECEPTORS, ADRENERGIC, BETA; SIGNAL TRANSDUCTION; VENTRICULAR FUNCTION, RIGHT;

EID: 0037840427     PISSN: 87507587     EISSN: None     Source Type: Journal    
DOI: 10.1152/japplphysiol.00958.2002     Document Type: Article

References (37)

1. Baker JE, Curry BD, Olinger GN, and Gross GJ. Increased tolerance of the chronically hypoxic immature heart to ischemia. Contribution of the KATP channel. Circulation 95: 1278-1285, 1997.
2. Cunningham WL, Becker EJ, and Kreuzer F. Catecholamines in plasma and urine at high altitude. J Appl Physiol 20: 607-610, 1965.
3. Gierschik P, Milligan G, Pines M, Goldsmith P, Codina J, Klee W, and Spiegel A. Use of specific antibodies to quantitate the guanine nucleotide-binding protein Go in brain. Proc Natl Acad Sci USA 83: 2258-2262, 1986.
4. Giesberts AN, van Ginneken M, Gorter G, Lapetina EG, Akkerman JW, and van Willigen G. Subcellular localization of alpha-subunits of trimeric G-proteins in human platelets. Biochem Biophys Res Commun 234: 439-444, 1997.
5. Ihnatovych I, Hejnova L, Kostrnova A, Mares P, Svoboda P, and Novotny J. Maturation of rat brain is accompanied by differential expression of the long and short splice variants of G(s)alpha protein: identification of cytosolic forms of G(s)alpha. J Neurochem 79: 88-97, 2001.
6. Kacimi R, Richalet JP, Corsin A, Abousahl I, and Crozatier B. Hypoxia-induced downregulation of beta-adrenergic receptors in rat heart. J Appl Physiol 73: 1377-1382, 1992.
7. Kacimi R, Moalic JM, Aldashev A, Vatner DE, Richalet JP, and Crozatier B. Differential regulation of G protein expression in rat hearts exposed to chronic hypoxia. Am J Physiol Heart Circ Physiol 269: H1865-H1873, 1995.
8. Kolar F. Cardioprotective effects of chronic hypoxia: relation to preconditioning. In: Myocardial Preconditioning, edited by Wainwright CL and Parratt JR. Austin, TX: Landes, 1996, p. 261-275.
9. Kolar F and Ostadal B. Right ventricular function in rats with hypoxic pulmonary hypertension. Pfügers Arch 419: 121-126, 1991.
10. Leon-Velarde F, Bourin MC, Germack R, Mohammadi K, Crozatier B, and Richalet JP. Differential alterations in cardiac adrenergic signaling in chronic hypoxia or norepinephrine infusion. Am J Physiol Regul Integr Comp Physiol 280: R274-R281, 2001.
11. Li HT, Honbo NY, and Karliner JS. Chronic hypoxia increases beta 1-adrenergic receptor mRNA and density but not signaling in neonatal rat cardiac myocytes. Circulation 94: 3303-3310, 1996.
12. Mader SL, Downing CL, and Van Lunteren E. Effect of age and hypoxia on beta-adrenergic receptors in rat heart. J Appl Physiol 71: 2094-2098, 1991.
13. Maher JT, Manchanda SC, Cymerman A, Wolfe DL, and Hartley LH. Cardiovascular responsiveness to beta-adrenergic stimulation and blockade in chronic hypoxia. Am J Physiol 228: 477-481, 1975.
14. Mardon K, Merlet P, Syrota A, and Maziere B. Effects of 5-day hypoxia on cardiac adrenergic neurotransmission in rats. J Appl Physiol 85: 890-897, 1998.
15. Marsh JD and Sweeney KA. Beta-adrenergic receptor regulation during hypoxia in intact cultured heart cells. Am J Physiol Heart Circ Physiol 256: H275-H281, 1989.
16. Mazzeo RS, Wolfel EE, Butterfield GE, and Reeves JT. Sympathetic response during 21 days at high altitude (4,300 m) as determined by urinary and arterial catecholamines. Metabolism 43: 1226-1232, 1994.
17. Mitchell FM, Griffiths SL, Saggerson ED, Houslay MD, Knowler JT, and Milligan G. Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3. Biochem J 262: 403-408, 1989.
18. Nielsen TB, Lad PM, Preston MS, and Rodbell M. Characteristics of the guanine nucleotide regulatory component of adenylate cyclase in human erythrocyte membranes. Biochim Biophys Acta 629: 143-155, 1980.
19. Novotny J, Bourova L, Kolar F, and Svoboda P. Membranebound and cytosolic forms of heterotrimeric G proteins in young and adult rat myocardium: influence of neonatal hypo- and hyperthyroidism. J Cell Biochem 82: 215-224, 2001.
20. Novotny J, Bourova L, Malkova O, Svoboda P, and Kolar F. G proteins, beta-adrenoreceptors and beta-adrenergic responsiveness in immature and adult rat ventricular myocardium: influence of neonatal hypo- and hyperthyroidism. J Mol Cell Cardiol 31: 761-772, 1999.
21. Novotny J, Gustafson B, Kvapil P, and Ransnas LA. Adenovirus infection of myocardial cells induces an enhanced sensitivity to beta-adrenergic agonists by increasing the concentration of the stimulatory G-protein. Biochem Mol Biol Int 34: 993-1001, 1994.
22. Novotny J and Svoboda P. The long (Gsα-L) and short (Gsα-S) variants of the stimulatory guanine nucleotide-binding protein. Do they behave in an identical way? J Mol Endocrinol 20: 163-173, 1998.
23. Ostadal B, Ostadalova I, Kolar F, Pelouch V, and Dhalla NS. Cardiac adaptation to chronic hypoxia. Adv Org Biol 6: 43-60, 1998.
24. sα and adenylyl cyclase cause β-adrenoceptor desensitization in chronically hypoxic rat hearts. Am J Physiol Cell Physiol 279: C1455-C1463, 2000.
25. i responses to κ-opioid receptor stimulation in the heart of chronically hypoxic rats. Am J Physiol Cell Physiol 279: C1483-C1494, 2000.
26. Pelouch V, Kolar F, Ost'adal B, Milerova M, Cihak R, and Widimsky J. Regression of chronic hypoxia-induced pulmonary hypertension, right ventricular hypertrophy, and fibrosis: effect of enalapril. Cardiovasc Drugs Ther 11: 177-185, 1997.
27. Poupa O, Krofta K, Prochazka J, and Turek Z. Acclimation to simulated high altitude and acute cardiac necrosis. Fed Proc 25: 1243-1246, 1966.
28. Ressl J, Urbanova D, Widimsky J, Ostadal B, Pelouch V, and Prochazka J. Reversibility of pulmonary hypertension and right ventricular hypertrophy induced by intermittent high altitude hypoxia in rats. Respiration 31: 38-46, 1974.
29. Rocha-Singh KJ, Honbo NY, and Karliner JS. Hypoxia and glucose independently regulate the beta-adrenergic receptor-adenylate cyclase system in cardiac myocytes. J Clin Invest 88: 204-213, 1991.
30. Roth DA, Urasawa K, Leiber D, Insel PA, and Hammond HK. A substantial proportion of cardiac Gs is not associated with the plasma membrane. FEBS Lett 296: 46-50, 1992.
31. Roth DA, White CD, Hamilton CD, Hall JL, and Stanley WC. Adrenergic desensitization in left ventricle from streptozotocin diabetic swine. J Mol Cell Cardiol 27: 2315-2325, 1995.
32. Rudolph U, Koesling D, Hinsch KD, Seifert R, Bigalke M, Schultz G, and Rosenthal W. G-protein alpha-subunits in cytosolic and membranous fractions of human neutrophils. Mol Cell Endocrinol 63: 143-153, 1989.
33. Sethi R, Dhalla KS, Shah KR, and Dhalla NS. Characterization of adenylyl cyclase in heart sarcolemma in the absence or presence of alamethicin. Mol Cell Biochem 119: 185-193, 1993.
34. Sternweis PC and Gilman AG. Reconstitution of catecholamine-sensitive adenylate cyclase. Reconstitution of the uncoupled variant of the S40 lymphoma cell. J Biol Chem 254: 3333-3340, 1979.
35. Thandroyen FT, Muntz K, Rosenbaum T, Ziman B, Willerson JT, and Buja LM. Beta-receptor-adenylate cyclase coupling in hypoxic neonatal rat ventricular myocytes. Am J Physiol Heart Circ Physiol 256: H1209-H1217, 1989.
36. Voelkel NF, Hegstrand L, Reeves JT, McMurty IF, and Molinoff PB. Effects of hypoxia on density of beta-adrenergic receptors. J Appl Physiol 50: 363-366, 1981.
37. White AA. Separation and purification of cyclic nucleotides by alumina column chromatography. Methods Enzymol 38: 41-46, 1974.