Cost-effective method to synthesize fluorescently labeled DNA size standards using cloned AFLP fragments

BioTechniques

Volumn 34, Issue 6, 2003, Pages 1146-1148

  Ueno, Saneyoshi ^a,b   Tsumura, Yoshihiko ^a   Washitani, Izumi ^c  

^a FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE   (Japan)

^b JAPAN WILDLIFE RESEARCH CENTER   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROPLAST DNA; DNA FRAGMENT; GENOMIC DNA; TAQ POLYMERASE;

AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ARTICLE; COST EFFECTIVENESS ANALYSIS; DNA STRUCTURE; ELECTROPHORESIS; FLUORESCENCE; GENOTYPE; MICROSATELLITE MARKER; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; STANDARD; SYNTHESIS;

EID: 0037839774     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03346bm05     Document Type: Article

References (5)

1. Brondani, R.P. and D. Grattapaglia. 2001. Cost-effective method to synthesize a fluorescent internal DNA standard for automated fragment sizing. BioTechniques 31:793-800.
2. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414.
3. Ueno, S., Y. Tsumura, and I. Washitani. Development of microsatellite markers in Primula sieboldii E. Morren, a threatened Japanese perennial herb. Conserv. Genet. (In Press.)
4. Southern, E.M. 1979. Measurement of DNA length by gel electrophoresis. Anal. Biochem. 100:319-323.
5. Magnuson, V.L., D.S. Ally, S.J. Nylund, Z.E. Karanjawala, J.B. Rayman, J.I. Knapp, A.L. Lowe, S. Ghosh, and F.S. Collins. 1996. Substrate nucleotide-determined non-templated addition of adenine by Taq DNA polymerase: implications for PCR-based genotyping and cloning. BioTechniques 21:700-709.