Cost-effective method to synthesize a fluorescent internal DNA standard for automated fragment sizing

BioTechniques

Volumn 31, Issue 4, 2001, Pages 793-800

  Brondani, R P V ^a   Grattapaglia, D ^a  

^a UNIVERSIDADE CATÓLICA DE BRASÍLIA   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMARKERS; CHLOROPHYLL; DNA;

MICROSATELLITES;

BIOTECHNOLOGY;

PLANT DNA;

ALLELE; AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; ARTICLE; CHLOROPLAST; DNA SEQUENCE; DNA SYNTHESIS; FLUORESCENCE; GENE AMPLIFICATION; GENOTYPE; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; STANDARD;

EID: 0034799089     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314st06     Document Type: Article

References (13)

1. Modification of the GeneScan 2500 fluorescent dye standard for accurate product sizing
2. Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophylla
3. Accurate determination of the number of CAG repeats in the Huntington disease gene using a sequence-specific internal DNA standard
4. A dedicated internal standard in fragment length analysis of hyperpolymorphic short tandem repeats
5. Methods for precise sizing, automated binning of alleles, and reduction of error rates in large-scale genotyping using fluorescently labeled dinucleotide markers
6. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-test-cross: Mapping strategy and RAPD markers
7. A simple method for automated allele binning in microsatellite markers
8. The use of fluorescence detection and internal lane standards to size PCR products automatically
9. Application of multiplex PCR and fluorescence-based, semi-automated allele sizing technology for genotyping plant genetic resources
10. Application of fluorescent dUTP on PCR and automated PCR product detection by using internal lane size standard
11. Universal primers for amplification of three non-coding regions of chloroplast DNA