Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology

Veterinarni Medicina

Volumn 48, Issue 4, 2003, Pages 108-112

  Tomanová, Katarína ^a   Klimes J ^a   Smola, J ^a   Husnik R ^a  

^a UNIVERSITY OF VETERINARY AND PHARMACEUTICAL SCIENCES   (Czech Republic)

Author keywords

Chronic diarrhoea; Dog; Indirect immunofluorescence; Lawsonia intracellularis; Nested PCR

Indexed keywords

DNA;

ANIMAL TISSUE; ARTICLE; BACTERIUM DETECTION; BLOOD SAMPLING; CHRONIC DIARRHEA; CLINICAL FEATURE; DNA DETERMINATION; DOG; ENTERITIS; HISTOPATHOLOGY; INTESTINE BIOPSY; LABORATORY TEST; LAWSONIA INTRACELLULARIS; NONHUMAN; POLYMERASE CHAIN REACTION; RECTUM; SEROLOGY; SPECIES DIFFERENCE; SYMPTOM;

ANIMALIA; BACTERIA (MICROORGANISMS); CANIS FAMILIARIS; LAWSONIA INTRACELLULARIS;

EID: 0037819643     PISSN: 03758427     EISSN: None     Source Type: Journal    
DOI: 10.17221/5757-VETMED     Document Type: Article

References (11)

1. Boom R., Sol C.J.A, Salimans M.M.M., Jansen C.L., Wertheim-Van Dillen P.M.E., Van Der Noordaa J. (1990): Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol., 28, 495-503
2. Collins J.E., Libal M.C. (1983): Proliferative enteritis in two pups. J. Am. Vet. Med. Assoc., 183, 886-889
3. Husník R., Klimeš J., Tomanová K., Smola J., Halouzka R., Tichý F., Brázdil J. (2003): Lawsonia intracellularis in a dog with inflammatory bowel disease. Vet. Med.-Czech., 48, 141-146
4. Jones G.F., Ward G.E., Murtaugh M.P., Lin G., Gebhart C.J. (1993): Enhanced detection of intracellular organism of swine proliferative enteritis, Ileal symbiont intracellularis, in feces by Polymerase chain reaction. J. Clin. Microbiol., 31, 2611-2615
5. Jordan D.M., Knittel J.P., Roof M.B., Schwartz K., Larson D., Hoffman L.J. (1999): Detection of Lawsonia intracellularis in swine using polymerase chain reaction methodology. J. Vet. Diagn. Invest., 11, 45-49
6. Knittel J.P., Jordan D.M., Schwarz K.J., Janke B.H., Roof M.B., McOrist S., Harris D.L. (1998): Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs. Am. J. Vet. Res., 59, 722-726
7. Lavoie J.P., Drolet R., Parsons D., Leguillette R., Sauvageau R., Shapiro J., Houle L., Hallé G., Gebhart C.J. (2000): Equine proliferative enteropathy: a cause of weight loss, colic, diarrhoea and hypoproteinaemia in foals on three breeding farms in Canada. Equine Vet. J., 32, 418-425
8. Lawson G.H.K., Gebhart C.J. (2000): Proliferative enteropathy. J. Comp. Pathol., 122, 77-100
9. Leblanc B., Fox J.G., Le Net J.L., Masson M.T., Picard A. (1993): Hyperplastic gastritis with intraepithelial Campylobacter-like organisms in a Beagle dog. Vet. Pathol., 30, 391-394
10. McOrist S., Gebhart C.J., Boid R., Barns S.M. (1995): Characterization of Lawsonia intracellularis gen. nov., sp. nov., the obligately intracellular bacterium of porcine proliferative enteropathy. Int. J. Syst. Bacteriol., 45, 820-825
11. Zhang P., Gebhart C.J., Burden D., Duhamel G.E. (2000): Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay (PCR-ELOSA). Mol. Cell Probes, 14, 101-108