Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment

Biophysical Journal

Volumn 84, Issue 6, 2003, Pages 4078-4086

  Rocheleau, Jonathan V ^a   Edidin, Michael ^b   Piston, David W ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; T LYMPHOCYTE RECEPTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ANISOTROPY; ANTIBODY SPECIFICITY; ANTIGEN PRESENTATION; ARTICLE; CELL MEMBRANE; COMPLEX FORMATION; CONTROLLED STUDY; DIFFUSION; ENDOPLASMIC RETICULUM; FLUORESCENCE; MEASUREMENT; MICROENVIRONMENT; MOUSE; NONHUMAN; POLARIZATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN TRANSPORT; STEADY STATE;

ANIMALIA; MAIZE CHLOROTIC MOTTLE VIRUS;

EID: 0037763890     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(03)75133-9     Document Type: Article

References (30)

1. Axelrod, D. 1979. Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys. J. 26:557-573.
2. Axelrod, D. 1989. Fluorescence polarization microscopy. Methods Cell Biol. 30:333-352.
3. Bai, A., and J. Forman. 1997. The effect of the proteasome inhibitor lactacystin on the presentation of transporter associated with antigen processing (TAP)-dependent and TAP-independent peptide epitopes by class I molecules. J. Immunol. 159:2139-2146.
4. Blackman, S. M., C. E. Cobb, A. H. Beth, and D. W. Piston. 1996. The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy. Biophys. J. 71:194-208.
5. Blackman, S. M., D. W. Piston, and A. H. Beth. 1998. Oligomeric state of human erythrocyte band 3 measured by fluorescence resonance energy homotransfer. Biophys. J. 75:1117-1130.
6. Clayton, A. H., Q. S. Hanley, D. J. Arndt-Jovin, V. Subramaniam, and T. M. Jovin. 2002. Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM). Biophys. J. 83:1631-1649.
7. Cresswell, P., N. Bangia, T. Dick, and G. Diedrich. 1999. The nature of the MHC class I peptide loading complex. Immunol. Rev. 172:21-28.
8. Day, P. M., J. W. Yewdell, A. Porgador, R. N. Germain, and J. R. Bennink. 1997. Direct delivery of exogenous MHC class I molecule-binding oligopeptides to the endoplasmic reticulum of viable cells. Proc. Natl. Acad. Sci. USA. 94:8064-8069.
9. Gautier, I., M. Tramier, C. Durieux, J. Coppey, R. B. Pansu, J. C. Nicolas, K. Kemnitz, and M. Coppey-Moisan. 2001. Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFT-tagged proteins. Biophys. J. 80:3000-3008.
10. Hink, M. A., R. A. Griep, J. W. Borst, A. van Hoek, M. H. Eppink, A. Schots, and A. J. Visser. 2000. Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein. J. Biol. Chem. 275:17556-17560.
11. Lewis, J. W., A. Neisig, J. Neefjes, and T. Elliott. 1996. Point mutations in the α2 domain of HLA-A2.1 define a functionally relevant interaction with TAP. Curr. Biol. 6:873-883.
12. Lewis, J. W., and T. Elliott. 1998. Evidence for successive peptide binding and quality control stages during MHC class I assembly. Curr. Biol. 8:717-720.
13. Marguet, D., E. T. Spiliotis, T. Pentcheva, M. Lebowitz, J. Schneck, and M. Edidin. 1999. Lateral diffusion of GFP-tagged H2Ld molecules and of GFP-TAP1 reports on the assembly and retention of these molecules in the endoplasmic reticulum. Immunity. 11:231-240.
14. Neisig, A., C. J. Melief, and J. Neefjes. 1998. Reduced cell surface expression of HLA-C molecules correlates with restricted peptide binding and stable TAP interaction. J. Immunol. 160:171-179.
15. Pamer, E., and P. Cresswell. 1998. Mechanisms of MHC class I-restricted antigen processing. Annu. Rev. Immunol. 16:323-358.
16. Patterson, G. H., D. W. Piston, and B. G. Barisas. 2000. Forster distances between green fluorescent protein pairs. Anal. Biochem. 284:438-440.
17. Pentcheva, T., and M. Edidin. 2001. Clustering of peptide-loaded MHC class I molecules for endoplasmic reticulum export imaged by fluorescence resonance energy transfer. J. Immunol. 166:6625-6632.
18. Pentcheva, T., E. T. Spiliotis, and M. Edidin. 2002. Cutting edge: tapasin is retained in the endoplasmic reticulum by dynamic clustering and exclusion from endoplasmic reticulum exit sites. J. Immunol. 168: 1538-1541.
19. Reddehase, M. J., J. B. Rothbard, and U. H. Koszinowski. 1989. A pentapeptide as minimal antigenic determinant for MHC class I-restricted T-lymphocytes. Nature. 337:651-653.
20. Saffman, P. G., and M. Delbrük. 1975. Brownian motion in biological membranes. Proc. Natl. Acad. Sci. USA. 72:3111-3113.
21. Spiliotis, E. T., H. Manley, M. Osorio, M. C. Zuniga, and M. Edidin. 2000. Selective export of MHC class I molecules from the ER after their dissociation from TAP. Immunity. 13:841-851.
22. Suh, W. K., M. F. Cohen-Doyle, K. Fruh, K. Wang, P. A. Peterson, and D. B. Williams. 1994. Interaction of MHC class I molecules with the transporter associated with antigen processing. Science. 264:1322-1326.
23. Suh, W. K., E. K. Mitchell, Y. Yang, P. A. Peterson, G. L. Waneck, and D. B. Williams. 1996. MHC class I molecules form ternary complexes with calnexin and TAP and undergo peptide-regulated interaction with TAP via their extracellular domains. J. Exp. Med. 184:337-348.
24. Swaminathan, R., C. P. Hoang, and A. S. Verkman. 1997. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys. J. 72:1900-1907.
25. Tank, D. W., E. S. Wu, and W. W. Webb. 1982. Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints. J. Cell Biol. 92:207-212.
26. Timbs, M. M., and N. L. Thompson. 1990. Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery. Biophys. J. 58:413-428.
27. van der Meer, B. W. 1999. Orientational aspects in pair energy transfer. In Resonance Energy Transfer. D. L. Andrews, and A. A. Demidov, editors. Wiley, New York.
28. Velez, M., and D. Axelrod. 1988. Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes. Biophys. J. 53:575-591.
29. Volkmer, A., V. Subramaniam, D. J. Birch, and T. M. Jovin. 2000. One-and two-photon excited fluorescence lifetimes and anisotropy decays of green fluorescent proteins. Biophys. J. 78:1589-1598.
30. Yuan, Y., and D. Axelrod. 1995. Subnanosecond polarized fluorescence photobleaching: rotational diffusion of acetylcholine receptors on developing muscle cells. Biophys. J. 69:690-700.