Effects of Ocimum gratissimum L. essential oil at subinhibitory concentrations on virulent and multidrug-resistant Shigella strains from Lagos, Nigeria

APMIS

Volumn 111, Issue 4, 2003, Pages 477-482

  Iwalokun, B A ^a   Gbenle, G O ^b   Adewole, T A ^c   Smith, S I ^c   Akinsinde, K A ^c   Omonigbehin, E O ^c  

^a LAGOS STATE UNIVERSITY   (Nigeria)

^b UNIVERSITY OF LAGOS   (Nigeria)

^c NIGERIAN INSTITUTE OF MEDICAL RESEARCH   (Nigeria)

Author keywords

Antibiotic susceptibility; Mechanism; Ocimum gratissimum essential oil (EO); Shigellae; Virulence

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; OCIMUM GRATISSIMUM EXTRACT; PROTEINASE; RHAMNOSE;

ANIMAL MODEL; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; GUINEA PIG; INHIBITION KINETICS; KERATOCONJUNCTIVITIS; MEDICINAL PLANT; MINIMUM INHIBITORY CONCENTRATION; MULTIDRUG RESISTANCE; NIGERIA; NONHUMAN; OCIMUM GRATISSIMUM; PRIORITY JOURNAL; SHIGELLA; SHIGELLOSIS; STATISTICAL ANALYSIS;

ANIMALS; COLORING AGENTS; CONGO RED; DRUG RESISTANCE, BACTERIAL; DYSENTERY, BACILLARY; ENDOPEPTIDASES; GUINEA PIGS; KERATOCONJUNCTIVITIS, INFECTIOUS; MICROBIAL SENSITIVITY TESTS; NIGERIA; OCIMUM; PLANT OILS; RHAMNOSE; SHIGELLA;

EID: 0037702776     PISSN: 09034641     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0463.2003.1110405.x     Document Type: Article

References (22)

1. Olukoya DK, Oni O. Plasmid profile analysis and antimicrobial susceptibility patterns of Shigella isolates from Nigeria. Epidemiol Infect 1990;105:59-64.
2. Niemogha MT, Alabi SA, Uzoma KL, Odugbemi TO, Adegbola RA, Coker AO, The incidence of Salmonella, Shigella and other enteric bacterial pathogens in stool specimens of diarrhoeic patients. Niger Med J 1995;28:70-4.
3. Iwalokun BA, Gbenle GO, Smith SI, Ogunledun A, Akinsinde KA, Omonigbehin EA. Epidemiology of shigellosis in Lagos, Nigeria: trends in antimicrobial resistance. J Health Popul Nutr 2001;19:183-90.
4. Janssen AM, Scheffer JJ, Ntezurubanza L, Baerheim Svendsen A. Antimicrobial activities of Ocimum species grown in Rwanda. J Ethnopharmacol 1989;26:57-63.
5. Jedlickova Z, Mottl O, Sery V. Antibacterial properties of the Vietnamese cajeput oil and ocimum oil in combination with antibacterial agents. J Hyg Epidemiol Microbiol Immunol 1992;36:303-9.
6. Ilori M, Sheteolu AO, Omonigbehin EA, Adeneye AA. Antidiarrhoeal activities of Ocimum gratissimum (Lamiaceae). J Diarhoeal Dis Res 1996;14:283-5.
7. Aziba PI, Bass D, Elegbe Y. Pharmacological investigation of Ocimum gratissimum in rodents. Phytother Res 1999;13:427-9.
8. Iwalokun BA, Gbenle GO, Adewole TA, Akinsinde KA. Shigellocidal properties of three Nigerian medicinal plants: Ocimum gratissimum, Terminalia avicennoides, Momordica balsamina. J Health Popul Nutr 2001;19:331-5.
9. Iwalokun BA, Gbenle GO, Adewole TA, Smith SI. Purification and partial characterization of 56 and 102 kDal proteases from a virulent Shigella flexneri strain isolated in Lagos, Nigeria. Afr J Med Pharm Sci 2001;3:102-7
10. Iwalokun BA, Gbenle GO, Adewole TA, Smith SI. A comparative study of virulence in Shigella isolates from Lagos, Nigeria. Bioscience Res Commun 2000;16:214-8.
11. Cowan ST Manual for identification of medical bacteria. 2nd ed. London: Cambridge University Press, 1974:104-5.
12. Nakamura CV, Ueda-Nakamura T, Bando E, Melo AF, Cortez DA, DiasFilho BP. Antibacterial activity of Ocimum gratissimum L. essential oil. Mem Inst Oswaldo Cruz 1999;5:675-8
13. Li E, Yousten A. A metalloprotease from Bacillus thuringiensis. Appl Microbiol 1975;30:354-5.
14. Quadri F, Hossain SA, Cisznar I, Haider K, Ljungh A, Wadstrom T, Sack DA. Congo red binding and salt aggregation as indicator of virulence in Shigella species. J Clin Microbiol 1988; 26:1343-8.
15. Sereny B. Experimental Shigella conjunctivitis. Acta Microbiological Academica Scientoforum Hungaricae 1955;2:293-6.
16. Westphal O, Jann K. Bacterial lipopolysaccharide extraction with phenol. Methods Carbohydr Chem 1965;5:83-91.
17. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric methods for determination of sugars and related substances. Anal Chem 1956;28:350.
18. National Committee for Clinical Laboratory Standards Methods for dilution. Antimicrobial susceptibility tests for bacteria that grow aerobically. Second edition 1990; Approved Standard. M7-A2. NCCLS, Villanova, PA, USA.
19. Sansonetti PJ. Molecular mechanisms of cell and tissue invasion by Shigella flexneri. Infect Agents Dis 1993;2:201-6.
20. Khaleda H, Hossain A, Wanke C, Qadri F, Ali S, Nahar S. Production of mucinase and neuraminidase and binding of Shigella to intestinal mucin. J Diarrhoeal Dis Res 1993;11:88-92.
21. AL-Hasani K, Henderson RJ, Sakellaris H, Rajakumar K, Grant T, Nataro JP, Robins-Browne R, Adler B. The sig A gene which is borne on the she pathogenicity island of Shigella flexneri 2a encodes an exported cytopathic protease involved in epithelial fluid accumulation. Infect Immun 2000;68:2457-63.
22. Fakae BB, Campbell AM, Barrett J, Scott IM, Teesdale-Spittle PH, Liebau E, Brophy PM. Inhibition of glutathione-s-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants. Phytother Res 2000; 14:630-4.