Identification of lysine 122 and arginine 196 as important functional residues of rat CTP:phosphocholine cytidylyltransferase alpha

Biochemistry

Volumn 42, Issue 17, 2003, Pages 5043-5051

  Helmink, Beth Ann ^a   Braker, Jay D ^a   Kent, Claudia ^b   Friesen, Jon A ^a  

^a ILLINOIS STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; MUTAGENESIS; PROTEINS; SYNTHESIS (CHEMICAL);

BUCALOVIRUS EXPRESSION SYSTEM;

AMINO ACIDS;

ARGININE; ARGINYLTHREONYLGLUTAMINYLGLYCYLISOLEUCYLSERYLTHREONINE; CHOLINE PHOSPHATE CYTIDYLYLTRANSFERASE; CHOLINE PHOSPHATE CYTIDYLYLTRANSFERASE ALPHA; CITICOLINE; CYTIDINE TRIPHOSPHATE; LYSINE; PEPTIDE; PHOSPHATIDYLCHOLINE; PHOSPHORYLCHOLINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME SUBSTRATE COMPLEX; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; RAT; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; BASE SEQUENCE; CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE; CONSERVED SEQUENCE; CYTIDINE TRIPHOSPHATE; DNA PRIMERS; KINETICS; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLCHOLINE; PROTEIN SUBUNITS; RATS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

ANIMALIA; UNIDENTIFIED BACULOVIRUS;

EID: 0037657645     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi027431+     Document Type: Article

References (29)

1. Vance, D. E., Trip, E. M., and Paddon, H. B. (1980) J. Biol. Chem. 256, 1064-1069.
2. Wilgram, G. F., and Kennedy, E. P. (1957) J. Biol. Chem. 227, 951-962.
3. Sleight, R., and Kent, C. (1980) J. Biol. Chem. 255, 10644-10650.
4. Kalmar, G. B., Kay, R. J., LaChance, A. C., and Cornell, R. B. (1994) Biochim. Biophys. Acta 1219, 328-224.
5. Lykidis, A., Murti, K. G., and Jackowski, S. (1998) J. Biol. Chem. 273, 14022-14029.
6. Kent, C. (1997) Biochim. Biophys. Acta 1348, 79-90.
7. Nakashima, A., Hosaka, K., and Nikawa, J. (1997) J. Biol. Chem. 272, 9567-9572.
8. Pooley, H. M., Abellan, F. X., and Karamato, D. (1991) J. Gen. Microbiol. 137, 921-928.
9. Park, Y. S., Gee, P., Sanker, S., Schurter, E. J., Zuiderweg, E. R. P., and Kent, C. (1997) J. Biol. Chem. 24, 15161-15166.
10. Veitch, D. P., Gilham, D., and Cornell, R. B. (1998) Eur. J. Biochem. 255, 227-234.
11. Weber, C., Park, Y. S., Sanker, S., Kent, C., and Ludwig, M. (1999) Structure 7, 1113-1124.
12. MacDonald, J. I. S., and Kent, C. (1993) Protein Expr. Purif. 4, 1-7.
13. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R. (1989) Gene 77, 51-59.
14. Friesen, J. A., Campbell, H. A., and Kent, C. (1999) J. Biol. Chem. 274, 13384-13389.
15. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254.
16. Segel, L. H. (1975) Enzyme Kinetics. Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, John Wiley & Sons, New York.
17. Comell, R. (1989) J. Biol. Chem. 264, 9077-9082.
18. Weinhold, P. A., Rounsifer, M. E., Charles, L., and Feldman, D. A. (1989) Biochim. Biophys. Acta 1006, 299-310.
19. Kwak, B. Y., Zhang, Y. M., Yum M., Heath, R. J., Rock, C. O., Jackowski, S., and Park, H. W. (2002) J. Biol. Chem. 277, 4343-4350.
20. Rock, C. O., Heath, R. J., Park, H. W., and Jackowski, S. (2001) J. Bacteriol. 183, 4927-4931.
21. Weinhold, P. A., Rounsifer, M. E., and Feldman, D. A. (1986) J. Biol. Chem. 261, 5104-5110.
22. Luche, M. M., Rock, C. O., and Jackowski, S. (1993) Arch. Biochem. Biophys. 301, 114-118.
23. Yang, W., Boggs, K. P., and Jackowski, S. (1995) J. Biol. Chem. 270, 23951-23957.
24. Bork, P., Holm, L., Koonin, E. V., and Sander, C. (1995) Proteins 22, 259-266.
25. Leatherbarrow, R. J., Fersht, A. R., and Winter, G. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 7840-7844.
26. Fersht, A. R., Knill-Jones, J. W., Bedouelle, H., and Winter, G. (1988) Biochemistry 27, 1581-1587.
27. Nureki, O., Vassylyev, D. G., Katayanagi, K., Shimizu, T., Sekine, S., Kigawa, T., Miyazawa, T., Yokoyama, S., and Morikawa, K. (1995) Science 267, 1958-1965.
28. Xin, Y., Li, W., and First, E. A. (2000) Biochemistry 39, 340-347.
29. Perona, J. J., Rould, M. A., and Steitz, T. A. (1993) Biochemistry 32, 8758-8771.