Inhibition of rho-associated kinase reduces MLC20 phosphorylation and contractility of intact myometrium and attenuates agonist-induced Ca2+ sensitization of force of permeabilized rat myometrium

Journal of Veterinary Medical Science

Volumn 65, Issue 1, 2003, Pages 43-50

  Oh, Jae Hwan ^b   You, Sung Kyung ^a   Hwang, Mi Kyung ^a   Ahn, Duck Sun ^a   Kwon, Seong Chun ^a   Taggart, Michael J ^b   Lee, Young Ho ^a  

^a Yonsei University   (South Korea)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

MLC20 phosphorylation; Rat myometrium; Rho associated kinase (ROK); rhoA; Y 27632

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; AMIDE; CALCIUM; CALPHOSTIN B; CARBACHOL; CHOLINERGIC RECEPTOR STIMULATING AGENT; ENZYME INHIBITOR; MYOSIN LIGHT CHAIN; NAPHTHALENE DERIVATIVE; PROTEIN SERINE THREONINE KINASE; PYRIDINE DERIVATIVE; RHO KINASE; RHO-ASSOCIATED KINASE; SIGNAL PEPTIDE;

ANIMAL; ARTICLE; DRUG ANTAGONISM; DRUG EFFECT; FEMALE; METABOLISM; MYOMETRIUM; PERMEABILITY; PHOSPHORYLATION; PHYSIOLOGY; PREGNANCY; RAT; SPRAGUE DAWLEY RAT; UTERUS CONTRACTION;

AMIDES; ANIMALS; CALCIUM; CARBACHOL; CHOLINERGIC AGENTS; ENZYME INHIBITORS; FEMALE; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MYOMETRIUM; MYOSIN LIGHT CHAINS; NAPHTHALENES; PERMEABILITY; PHOSPHORYLATION; PREGNANCY; PROTEIN-SERINE-THREONINE KINASES; PYRIDINES; RATS; RATS, SPRAGUE-DAWLEY; UTERINE CONTRACTION;

EID: 0037643529     PISSN: 09167250     EISSN: None     Source Type: Journal    
DOI: 10.1292/jvms.65.43     Document Type: Article

References (26)

1. Taggart, M.J. and Wray, S. 1998. Contribution of sarcoplasmic reticular calcium to smooth muscle contractile activation: gestational-dependence in isolated rat uterus. J. Physiol. 511: 133-144.
2. i, myosin light chain phosphorylation and contraction in human myometrium. Am. J. Physiol. 260: C861-C867.
3. 2+, phosphorylation of myosin regulatory light chain and force in isolated rat smooth muscle. J. Physiol. 499: 485-496.
4. Khromov, A., Somlyo, A.V., Trentham, D.R., Zimmermann, B. and Somlyo, A.P. 1995. The role of MgADP in force maintenance by dephosphorylated cross-bridge in smooth muscle: a flash photolysis study. Biophys. J. 69: 2611-2622.
5. 2+ in pregnant rat myometrium. Am. J. Obstet. Gynecol. 175: 199-206.
6. Somlyo, A.P. and Somlyo, A.V. 1998. From pharmacomechanical coupling to G-proteins and myosin phosphatase. Acta Physiol. Scand. 164: 437-448.
7. Somlyo, A.P, and Somlyo, A.V. 2000. Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II. J. Physiol. 522: 177-185.
8. Kimura, K., Ito, M., Amano, M., Chihara, K., Fukata, Y., Nakafuku, M., Yamamori, B., Feng, J., Nakano, T., Okawa, K., Iwamatsu, A. and Kaibuchi, K. 1996. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248.
9. Izumi, H., Byam-Smith, M. and Garfield, R.E. 1995. Gestational changes in oxytocin- and endothelin-1-induced contractility of pregnant rat myometrium. Eur. J. Pharmacol. 278: 187-194.
10. Lee, Y.-H., Hwang, M.-K., Morgan, K.G. and Taggart, M.J. 2001. Receptor-coupled contractility of uterine smooth muscle: from membrane to myofilaments. Exp. Physiol. 86: 283-288.
11. Niiro, N., Nishimura, J., Sakiha, C., Nakano, H. and Kanaide, H. 1997. Up-regulation of rho A and rho-kinase mRNAs in the rat myometrium during pregnancy. Biochem. Biophys. Res. Commun. 230: 356-359.
12. Moore, F., Da Silva, C., Wilde, J.L, Smarason, A., Watsom, S.P. and Lopez Bernal, A. 2000. Up-regulation of p21- and Rho a-activated protein kinases in human pregnant Myometrium. Biochem. Biophys. Res. Comm. 269: 322-326.
13. Taggart, M.J., Lee, Y.-H. and Morgan, K.G. 1999. Cellular redistribution of PKCα, rhoA, and ROKα following smooth muscle agonist stimulation. Exp. Cell. Res. 251: 92-101.
14. 2+, and tension in intact smooth muscles. Am. J. Physiol. 260: C917-C925.
15. Lee, Y.-H., Kim, I., Laporte, R., Walsh, M.P. and Morgan, K.G. 1999. Isozyme-specific inhibitors of protein kinase C translocation: effects on contractility of single permeabilized vascular muscle cells of the ferret. J. Physiol. 517: 709-720.
16. Brozovich, F.V., Yates, L.D. and Gordon, A.M. 1988. Muscle force and stiffness during activation and relaxation. Implications for actomyosin ATPase. J. Gen. Physiol. 91: 399-420.
17. Uehata, M., Ishizaki, T., Satoh, H., Ono, T., Kawahara, T., Morishita, T., Tamakawa, H., Yamagami, K., Inui, J., Maekawa, M. and Narumiya, S. 1997. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature (Lond.) 389: 990-994.
18. Crichton, C.A., Taggart, M.J., Wray, S. and Smith, G.L. 1993. The effect of pH and inorganic phosphate on force production in alpha-toxin permeabilised isolated rat uterine smooth muscle. J. Physiol. 465: 629-646.
19. 2+ sensitisation of smooth muscle. Proc. Natl. Acad. Sci. 93: 1340-1345.
20. Fujihara, K., Walker, L.A., Gong, M.C., Lemichez, E., Boquet, P., Somlyo, A.V. and Somlyo, A.P. 1997. Inhibition of rhoA translocation and calcium sensitization by in vivo ADP-ribosylation with the chimeric toxin DC3B. Mol. Biol. Cell 8: 2437-2447.
21. 2+ sensitisation of myosin phosphorylation and force in guinea-pig ileum. J. Physiol. 522: 33-49.
22. Otto, B., Steusloff, A., Scherer, P.E. and Pfitzer, G. 1996. Role of Rho proteins in carbachol-induced contraction in intact and permeabilized guinea-pig intestinal smooth muscle. J. Physiol. 496: 317-329.
23. Yu J.T.H. and Lopez Bernal, A. 1996. The cytoskeleton of human myometrial cells. J. Reprod. Fertil. 112: 185-198.
24. Taggart, M.J., Leavis, P., Feron, O. and Morgan, K.G. 2000. Inhibition of PKCα and rhoA translocation in differentiated smooth muscle by a caveolin scaffolding domain peptide. Exp. Cell Res. 258: 72-81.
25. Amano, M., Ito, M., Kimura, K., Fukata, Y., Chihara, K., Nakano, T., Matsuura, Y. and Kaibuchi, K. 1996. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase). J. Biol. Chem. 271: 20246-20249.
26. Kureishi, Y., Kobayashi, S., Amano, M., Kimura, K., Kanaide, H., Nakano, T., Kaibuchi, K. and Ito, M. 1997. Rho-associated kinase directly induces smooth muscle contraction through myosin light chain phosphorylation. J. Biol. Chem. 272: 12257-12260.