Molecular basis for fungal selectivity of novel antimitotic compounds

Antimicrobial Agents and Chemotherapy

Volumn 47, Issue 7, 2003, Pages 2273-2282

  Lila, Thomas ^a,b   Renau, Thomas E ^a,c   Wilson, Lori ^a   Philips, Jay ^a,d   Natsoulis, Georges ^a,e   Cope, M Jamie ^a,f   Watkins, W ^a   Buysse, Jerry ^a,f  

^a Essential Therapeutics Inc   (United States)

^b Cytokinetics Inc   (United States)

^c ROCHE BIOSCIENCE   (United States)

^d EPIGENOMICS AG   (Germany)

^e Entelos Inc   (United States)

^f Symyx Therapeutics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ANTIMITOTIC AGENT; BENOMYL; BETA TUBULIN; CERULENIN; COLCHICINE; CYCLOHEXIMIDE; FLUCONAZOLE; MC 05905; MC 05991; MC 06011; MC 06285; MC 06307; MC 06341; MC 06380; MC 226728; MC 239300; MC 247136; MC 253166; MC 253637; MC 291734; MC 305904; NOCODAZOLE; TIABENDAZOLE; TUBULIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIFUNGAL ACTIVITY; ARTICLE; BINDING SITE; DRUG SELECTIVITY; GROWTH INHIBITION; HUMAN; HUMAN CELL; MICROTUBULE ASSEMBLY; MINIMUM INHIBITORY CONCENTRATION; MITOSIS INHIBITION; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEST CONTROL; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION; SYSTEMIC MYCOSIS;

ANTIFUNGAL AGENTS; BENZIMIDAZOLES; BINDING SITES; DRUG DESIGN; MICROBIAL SENSITIVITY TESTS; MUTATION; POLYMERS; SACCHAROMYCES CEREVISIAE; TUBULIN;

EID: 0037636479     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.47.7.2273-2282.2003     Document Type: Article

References (26)

1. Adams, A., D. Gottschling, C. Kaiser, and T. Stearns. 1997. Methods in yeast genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
2. Amos, L. A., and J. Lowe. 1999. How Taxol stabilises microtubule structure. Chem. Biol. 6:R65-R69.
3. Bai, R., D. G. Covell, X. F. Pei, J. B. Ewell, N. Y. Nguyen, A. Brossi, and E. Hamel. 2000. Mapping the binding site of colchicinoids on beta-tubulin. 2-Chloroacetyl-2-demethylthiocolchicine covalently reacts predominantly with cysteine 239 and secondarily with cysteine 354. J. Biol. Chem. 275:40443-40452.
4. Barnes, G., K. A. Louie, and D. Botstein. 1992. Yeast proteins associated with microtubules in vitro and in vivo. Mol. Biol. Cell 3:29-47.
5. Bode, C. J., M. L. Gupta, Jr., E. A. Reiff, K. A. Suprenant, G. I. Georg, and R. H. Himes. 2002. Epothilone and paclitaxel: unexpected differences in promoting the assembly and stabilization of yeast microtubules. Biochemistry 41:3870-3874.
6. Davis, A., C. R. Sage, L. Wilson, and K. W. Farrell. 1993. Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae. Biochemistry 32:8823-8835.
7. Fonzi, W. A., and M. Y. Imin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
8. Fujimura, M., K. Oeda, H. Inoue, and T. Kato. 1992. A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity. Curr. Genet. 21:399-404.
9. Haggarty, S. J., T. U. Mayer, D. T. Miyamoto, R. Fathi, R. W. King, T. J. Mitchison, and S. L. Schreiber. 2000. Dissecting cellular processes using small molecules: identification of colchicine-like, taxol-like and other small molecules that perturb mitosis. Chem. Biol. 7:275-286.
10. Jung, M. K., and B. R. Oakley. 1990. Identification of an amino acid substitution in the benA, beta-tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity. Cell Motil. Cytoskeleton 17:87-94.
11. Jung, M. K., I. B. Wilder, and B. R. Oakley. 1992. Amino acid alterations in the benA (beta-tubulin) gene of Aspergillus nidulans that confer benomyl resistance. Cell Motil. Cytoskeleton 22:170-174.
12. Kilmartin, J. V. 1981. Purification of yeast tubulin by self-assembly in vitro. Biochemistry 20:3629-3633.
13. Li, J., S. K. Katiyar, and T. D. Edlind. 1996. Site-directed mutagenesis of Saccharomyces cerevisiae beta-tubulin: interaction between residue 167 and benzimidazole compounds, FEBS Lett. 385:7-10.
14. Lowe, J., H. Li, K. H. Downing, and E. Nogales. 2001. Refined structure of alpha beta-tubulin at 3.5 A resolution. J. Mol. Biol. 313:1045-1057.
15. Machin, N. A., J. M. Lee, and G. Barnes. 1995. Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol. Biol. Cell 6:1241-1259.
16. Mayer, T. U., T. M. Kapoor, S. J. Haggarty, R. W. King, S. L. Schreiber, and T. J. Mitchison. 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 286:971-974.
17. Nogales, E., S. G. Wolf, I. A. Khan, R. F. Luduena, and K. H. Downing. 1995. Structure of tubulin at 6.5 A and location of the taxol-binding site. Nature 375:424-427.
18. Paull, K. D., C. M. Lin, L. Malspeis, and E. Hamel. 1992. Identification of novel antimitotic agents acting at the tubulin level by computer-assisted evaluation of differential cytotoxicity data. Cancer Res. 52:3892-3900.
19. Prichard, R. 2001. Genetic variability following selection of Haemonchus contortus with anthelmintics. Trends Parasitol. 17:445-453.
20. Rai, S. S., and J. Wolff. 1996. Localization of the vinblastine-binding site on beta-tubulin. J. Biol. Chem. 271:14707-14711.
21. Reijo, R. A., E. M. Cooper, G. J. Beagle, and T. C. Huffaker. 1994. Systematic mutational analysis of the yeast beta-tubulin gene. Mol. Biol. Cell 5:29-43.
22. Richards, K. L., K. R. Anders, E. Nogales, K. Schwartz, K. H. Downing, and D. Botstein. 2000. Structure-function relationships in yeast tubulins. Mol. Biol. Cell 11:1887-1903.
23. Sali, A., and T. L. Blundell. 1990. Definition of general topological equivalence in protein structures. A procedure involving comparison of properties and relationships through simulated annealing and dynamic programming. J. Mol. Biol. 212:403-428.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
26. Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.