Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry

ChemBioChem

Volumn 4, Issue 5, 2003, Pages 396-405

  Jose, Joachim ^a   Handel, Steffen ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Autodisplay; Fluorescence; Maleimide; Michael addition; Protein engineering

Indexed keywords

BACTERIAL PROTEIN; CYSTEINE; MALEIMIDE; MALEIMIDE DERIVATIVE; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

ANALYTIC METHOD; ARTICLE; CELL LABELING; CELL SURFACE; COVALENT BOND; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; MICHAEL ADDITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; SPECTROPHOTOMETRY; WESTERN BLOTTING;

ADRENODOXIN; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; BIOTIN; CATTLE; CYSTEINE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; FLUORESCEIN; MALEIMIDES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037573639     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200200530     Document Type: Article

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