Immunocytochemical localization of Na,K-ATPase gamma subunit and CHIF in inner medulla of rat kidney

Annals of the New York Academy of Sciences

Volumn 986, Issue , 2003, Pages 401-409

  Pihakaski Maunsbach, Kaarina ^a   Vorum, Henrik ^a   Løcke, Else Merete ^a   Garty, Haim ^b   Karlish, Steven J D ^b   Maunsbach, Arvid B ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

CHIF; Collecting duct; Gamma subunit; Intercalated cells; Kidney inner medulla; Principal cells

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ANTIBODY; CORTICOSTEROID HORMONE INDUCED FACTOR; PROTEIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; BASOLATERAL MEMBRANE; CARBOXY TERMINAL SEQUENCE; CELL LABELING; CELL MEMBRANE; CELL SURFACE; CELLULAR DISTRIBUTION; CONFERENCE PAPER; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE; KIDNEY MEDULLA; LASER; MALE; NONHUMAN; PROTEIN LOCALIZATION; RAT; STAINING; WESTERN BLOTTING;

ANIMALIA;

EID: 0037564507     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.2003.tb07221.x     Document Type: Conference Paper

References (20)

1. FORBUSH, B., III, J.H. KAPLAN & J.F. HOFFMAN. 1978. Characterization of a new photo-affinity derivative of ouabain: labeling of the large polypeptide and of a proteolipid component of the Na,K-ATPase. Biochemistry 17: 3667-3676.
2. MERCER, R.W., D. BIEMESDERFER, D.P. BLISS, JR., et al. 1993. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase. J. Cell Biol. 121: 579-586.
3. SWEADNER, K.J. & E. RAEL. 2000. The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression. Genomics 68: 41-56.
4. + channel activity in Xenopus oocytes. Proc. Natl. Acad. Sci. USA 92: 6092-6096.
5. GARTY, H., M. LINDZEN, R. SCANZANO, et al. 2002. A functional interaction between CHIF and Na-K-ATPase: implication for regulation by FXYD proteins. Am. J. Physiol. Renal Physiol. 283: F607-F615.
6. KÜSTER, B., A. SHAINSKAYA, H.X. PU, et al. 2000. A new variant of the γ subunit of renal Na,K-ATPase: identification by mass spectrometry, antibody binding, and expression in cultured cells. J. Biol. Chem. 275:18441-18446.
7. Pu, H.X., F. CLUZEAUD, R. GOLDSHLEGER, et al. 2001. Functional role and immunocytochemical localization of the γa and γb forms of the Na,K-ATPase γ subunit. J. Biol. Chem. 276: 20370-20378.
8. WETZEL, R.K. & K.J. SWEADNER. 2001. Immunocytochemical localization of Na-K-ATPase α and γ subunits in rat kidney. Am. J. Physiol. Renal Physiol. 281: F531-F545.
9. ARYSTARKHOVA, E., R.K. WETZEL & K.J. SWEADNER. 2002. Distribution and oligomeric association of splice forms of Na(+)-K(+)-ATPase regulatory gamma-subunit in rat kidney. Am. J. Physiol. Renal Physiol. 282: F393-F407.
10. BÉGUIN, P., X. WANG, D. FIRSOV, et al. 1997. The gamma subunit is a specific component of the Na,K-ATPase and modulates its transport function. EMBO J. 16: 4250-4260.
11. THERIEN, A.G., S.J.D. KARLISH & R. BLOSTEIN. 1999. Expression and functional role of the γ subunit of the Na,K-ATPase in mammalian cells. J. Biol. Chem. 274: 12252-12256.
12. THERIEN, A.G., R. GOLDSHLEGER, S.J.D. KARLISH, et al. 1997. Tissue-specific distribution and modulatory role of the gamma subunit of the Na,K-ATPase. J. Biol. Chem. 272: 32628-32634.
13. + affinity of the renal Na,K-ATPase. J. Biol. Chem. 274 33183-33185.
14. CAPURRO, C., N. COUNTRY, J.P. BONVALET, et al. 1996. Cellular localization and regulation of CHIF in kidney and colon. Am. J. Physiol. 271: C753-C762.
15. SHI, H., R. LEVY-HOLZMAN, F. CLUZEAUD, et al. 2001. Membrane topology and immuno-localization of CHIF in kidney and intestine. Am. J. Physiol. Renal Physiol. 280: F505-F512.
16. BÉGUIN, P., G. CRAMBERT, S. GUENNOUN, et al. 2001. CHIF, a member of the FXYD protein family, is a regulator of Na,K-ATPase distinct from the gamma-subunit. EMBO J. 20: 3993-4002.
17. MAUNSBACH, A.B. 1998. Immunolabeling and staining of ultrathin sections in biological electron microscopy. In A Laboratory Handbook, J.E. Celis, Ed.: 268-276. Academic Press. San Diego.
18. CAPASSO, J., C. RIVARD & T. BERL. 2001. The expression of the γ subunit of Na, K-ATPase is regulated by osmolarity via C-terminal Jun kinase and phosphatidylinositol 3-kinase-dependent mechanisms. Proc. Natl. Acad. Sci. USA 98: 13414-13419.
19. SABOLIC, I., C.M. HERAK-KRAMBERGER, S. BRETON, et al. 1999. Na/K-ATPase in intercalated cells along the rat nephron revealed by antigen retrieval. J. Am. Soc. Nephrol. 10: 913-922.
20. Pu, H.X., R. SCANZANO & R. BLOSTEIN. 2002. Distinct regulatory effects of the Na,K-ATPase gamma subunit. J. Biol. Chem. 277: 20270-20276.