A novel enzyme, D-3-hydroxyaspartate aldolase from Paracoccus denitrificans IFO 13301: Purification, characterization, and gene cloning

Applied Microbiology and Biotechnology

Volumn 62, Issue 1, 2003, Pages 53-60

  Liu, J Q ^a,c   Dairi, T ^a   Itoh, N ^a   Kataoka, M ^b   Shimizu, S ^b  

^a TOYAMA PREFECTURAL UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c Kobe Technical Center   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYASPARTIC ACID; 3,4 DIHYDROXYPHENYLSERINE; BACTERIAL ENZYME; DEXTRO 3 HYDROXYASPARTATE ALDOLASE; PHENYLSERINE; THREONINE; UNCLASSIFIED DRUG;

ENZYME;

ACHROMOBACTER XYLOSOXIDANS; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PARACOCCUS DENITRIFICANS;

AMINO ACID SEQUENCE; ASPARTIC ACID; BASE SEQUENCE; CLONING, MOLECULAR; FRUCTOSE-BISPHOSPHATE ALDOLASE; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PARACOCCUS DENITRIFICANS; SUBSTRATE SPECIFICITY; TEMPERATURE;

ACHROMOBACTER XYLOSOXIDANS; ALCALIGENES; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PARACOCCUS DENITRIFICANS; PROKARYOTA;

EID: 0037560076     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1238-2     Document Type: Article

References (17)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
2. Balcar VJ, Jonston GA, Twitchin B (1977) Stereospecificity of the inhibition of L-glutamate and L-aspartate high affinity uptake in rat brain slices by threo-3-hydroxyaspartate. J Neurochem 28:1145-1146
3. Gibbs RG, Morris JG (1964) Assay and properties of erythro-β-hydroxyasparte aldolase from Micrococcus denitrificans. Biochim Biochem Acta 85:501-503
4. Gibbs RG, Morris JG (1965) Purification and properties of erythro-β-hydroxyasparte dehydratase from Micrococcus denitrificans. Biochem J 97:547-554
5. Higgins DG, Thompson JD, Gibson TJ (1996) Using CLUSTAL for multiple sequence alignments. Methods Enzymol 66:383-402
6. Ishiyama T, Furuta T, Takai M, Okimoto Y (1975) L-threo-β-hydroxyaspartate acid as an antibiotic amino acid. J Antibiotics 23:821-823
7. Kaneko T, Katsura H (1963) The synthesis of four optical isomers of β-hydroxyaspartatic acid. Bull Chem Soc Jpn 36:899-930
8. Kornberg HL, Morris JG (1965) The utilization of glycollate by Micrococcus denitrificans: the β-hydroxyaspartate pathway. Biochem J 95:577-586
9. Lebrun B, Sakitani M, Shimamoto K, Yasuda-Kamatani Y, Nakajima T (1997) New β-hydroxyaspartate derivatives are competitive blockers for the bovine glutamate/aspartate transporter. J Biol Chem 272:20336-20339
10. Liu JQ, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H (1998) Novel metal-activated pyridoxal enzyme with a unique primary structure, low-specificity D-threonine aldolase from Arthrobacter sp. strain DK-38. J Biol Chem 273:16678-16685
11. Liu JQ, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H (2000a) Diversity of microbial threonine aldolasees and their application. J Mol Cat B Enzymatic 10:107-115
12. Liu JQ, Otani M, Dairi T, Itoh N, Kataoka M, Shimizu S, Yamada H (2000b) Gene cloning and overproduction of low-specificity D-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drug. Appl Microbiol Biotechnol 54:44-51
13. Ohashi N, Nagata S, Ishizumi K, Maeshima K (1984) European Patent 83,300,059
14. Pearson WR, Lipman DJ (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444-2448
15. Saito H, Miura K (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619-629
16. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
17. Shimamoto K, Shigeri Y, Yasuda-Kamatani Y, Lebrun B, Yumoto N, Nakajima T (2000) Syntheses of optically pure β-hydroxyaspartate derivatives as glutamate transporter blockers. Bioorg Med Chem Lett 10:2407-2410