Pharmacological characterization of recombinant bovine α3β4 neuronal nicotinic receptors stably expressed in HEK 293 cells

Neuroscience Letters

Volumn 343, Issue 3, 2003, Pages 180-184

  Free, R Benjamin ^a   Von Fischer, Nathaniel D ^a   Boyd, R Thomas ^b   McKay, Dennis B ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b THE OHIO STATE UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

3 4; Adrenal; Binding; Epibatidine; HEK 293 cells; Nicotinic acetylcholine receptor

Indexed keywords

1,1 DIMETHYL 4 PHENYLPIPERAZINIUM; CARBACHOL; CHOLINERGIC RECEPTOR BLOCKING AGENT; CHOLINERGIC RECEPTOR STIMULATING AGENT; CYTISINE; DECAMETHONIUM; DIHYDRO BETA ERYTHROIDINE; EPIBATIDINE; METHYLLYCACONITINE; NICOTINE; NICOTINIC RECEPTOR; RECEPTOR SUBUNIT; RECOMBINANT RECEPTOR; TUBOCURARINE CHLORIDE; DIAGNOSTIC AGENT; FUSED HETEROCYCLIC RINGS; LIGAND; MESSENGER RNA; NICOTINIC AGENT; NICOTINIC RECEPTOR ALPHA3BETA4; PYRIDINE DERIVATIVE; RECOMBINANT PROTEIN;

ADRENAL MEDULLA; ANIMAL CELL; ARTICLE; BINDING COMPETITION; CELL MEMBRANE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG RECEPTOR BINDING; EMBRYO; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; ANIMAL; BIOSYNTHESIS; CATTLE; CELL LINE; DOSE RESPONSE; DRUG EFFECT; GENETICS; KINETICS; METABOLISM; NORTHERN BLOTTING;

ANIMALS; BICYCLO COMPOUNDS, HETEROCYCLIC; BINDING, COMPETITIVE; BLOTTING, NORTHERN; CATTLE; CELL LINE; CELL MEMBRANE; CHOLINERGIC AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; KINETICS; LIGANDS; NICOTINIC AGONISTS; PYRIDINES; RECEPTORS, NICOTINIC; RECOMBINANT PROTEINS; RNA, MESSENGER;

EID: 0037534293     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(03)00381-1     Document Type: Article

References (19)

1. Campos-Caro A., Smillie F.I., Dominguez del Toro E., Rovira J.C., Vicente-Agullo F., Chapuli J., Juiz J.M., Sala S., Sala F., Ballesta J.J., Criado M. Neuronal nicotinic acetylcholine receptors on bovine chromaffin cells: cloning, expression, and genomic organization of receptor subunits. J. Neurochem. 68:1997;488-497.
2. 50) of an enzymatic reaction. Biochem. Pharmacol. 22:1973;3099-3108.
3. Conroy W.G., Vernallis A.B., Berg D.K. The α5 gene product assembles with multiple acetylcholine receptor subunits to form distinctive receptor subtypes in brain. Neuron. 9:1992;679-691.
4. Criado M., Alamo L., Navarro A. Primary structure of an agonist binding subunit of the nicotinic acetylcholine receptor from bovine adrenal chromaffin cells. Neurochem. Res. 17:1992;281-287.
5. Di Angelantonio S., Nistri A., Moretti M., Clementi F., Gotti C. Antagonism of nicotinic receptors of rat chromaffin cells by N,N,N-trimethyl-1-(4-trans-stilbenoxy)-2-propylammonium iodide: a patch clamp and ligand binding study. Br. J. Pharmacol. 129:2000;1771-1779.
6. Flores C.M., DeCamp R.M., Kilo S., Rogers S.W., Hargreaves K.M. Neuronal nicotinic receptor expression in sensory neurons of the rat trigeminal ganglion: demonstration of α3β4, a novel subtype in the mammalian nervous system. J. Neurosci. 16:1996;7892-7901.
7. 3H]Epibatidine binding to bovine adrenal medulla: evidence for α3β4* nicotinic receptors. Neurosci. Lett. 318:2002;98-102.
8. Free R.B., McKay D.B. Receptor protection studies to characterize neuronal nicotinic receptors: tubocurarine prevents alkylation of adrenal nicotinic receptors. Brain Res. 891:2001;176-184.
9. Garcia-Guzman M., Sala F., Sala S., Campos-Caro A., Stuhmer W., Gutierrez L.M., Criado M. α-Bungarotoxin-sensitive nicotinic receptors on bovine chromaffin cells: molecular cloning, functional expression and alternative splicing of the α7 subunit. Eur. J. Neurosci. 7:1995;647-655.
10. Gerzanich V., Peng X., Wang F., Anand R., Fletcher S., Lindstrom J. Comparative pharmacology of epibatidine: a potent agonist for neuronal nicotinic acetylcholine receptors. Mol. Pharmacol. 48:1995;774-782.
11. ++ modulation of human neuronal α3 nicotinic receptors. J. Pharmacol. Exp. Ther. 286:1998;311-320.
12. Gu H., Wenger B.W., Lopez I., McKay S.B., Boyd R.T., McKay D.B. Characterization and localization of adrenal nicotinic receptors: evidence that mAb35-nicotinic receptors are the principal receptors mediating adrenal catecholamine release. J. Neurochem. 66:1996;1454-1461.
13. Lopez M.G., Montiel C., Herrero C.J., Garcia-Palomero E., Mayorgas I., Hernandez-Guijo J.M., Villarroya M., Olivares R., Gandia L., McIntosh J.M., Olivera B.M., Garcia A.G. Unmasking the functions of the chromaffin cell α7 nicotinic receptor by using short pulses of acetylcholine and selective blockers. Proc. Natl. Acad. Sci. USA. 95:1998;14184-14189.
14. Lukas R.J., Changeux J.-P., le Novère N., Albuquerque E.X., Balfour J.K., Berg D.K., Bertrand D., Chiappinelli V.A., Clarke P.B.S., Collins A.C., Dani J.A., Grady S.R., Kellar K.J., Lindstrom J.M., Marks M.J., Quik M., Taylor P.W., Wonnacott S. International Union of Pharmacology. XX. Current status of the nomenclature for nicotinic acetylcholine receptors and their subunits. Pharmacol. Rev. 51:1999;397-401.
15. Stauderman K.A., Mahaffy L.S., Akong M., Velicelebi G., Chavez-Noriega L.E., Crona J.H., Johnson E.C., Elliot K.J., Gillespie A., Reid M.T., Adams P., Harpold M.M., Corey-Naeve J. Characterization of human recombinant neuronal nicotinic acetylcholine receptors subunit combinations of α2β4, α3β4 and α4β4 stably expressed in HEK293 cells. J. Pharmacol. Exp. Ther. 284:1998;777-789.
16. Vernallis A.B., Conroy W.G., Berg D.K. Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes. Neuron. 10:1993;451-464.
17. Wenger B.W., Bryant D.L., Boyd R.T., McKay D.B. Evidence for spare nicotinic acetylcholine receptors and a β4 subunit in bovine adrenal chromaffin cells: studies using bromoacetylcholine, epibatidine, cytisine, and mAb35. J. Pharmacol. Exp. Ther. 281:1997;905-913.
18. Wilson S.P., Kirshner N. The acetylcholine receptor of the adrenal medulla. J. Neurochem. 28:1977;687-695.
19. Xiao Y., Meyer E.L., Thompson J.M., Surin A., Wroblewski J., Kellar K.J. Rat α3/β4 subtype of neuronal nicotinic acetylcholine receptor stably expressed in a transfected cell line: pharmacology of ligand binding and function. Mol. Pharmacol. 54:1998;322-333.