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Volumn 125, Issue 4, 2003, Pages 856-857

DNA polymerase-mediated DNA synthesis on a TNA template

Author keywords

[No Author keywords available]

Indexed keywords

3',2' ALPHA THREOSE NUCLEIC ACID; DNA POLYMERASE; MANGANESE; NUCLEIC ACID; PRIMER DNA; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

EID: 0037471633     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja028589k     Document Type: Article
Times cited : (101)

References (23)
  • 3
    • 0034680495 scopus 로고    scopus 로고
    • (a) Orgel, L. E. Science 2000, 290, 1306-1307.
    • (2000) Science , vol.290 , pp. 1306-1307
    • Orgel, L.E.1
  • 4
    • 0037062978 scopus 로고    scopus 로고
    • (b) Joyce, G. F. Nature 2002, 418, 214-221.
    • (2002) Nature , vol.418 , pp. 214-221
    • Joyce, G.F.1
  • 10
    • 0013003353 scopus 로고    scopus 로고
    • note
    • The assay and oligonucleotides used in this study were designed by Gerald F. Joyce.
  • 11
    • 0013006670 scopus 로고    scopus 로고
    • note
    • Recombinant DNA polymerases activities and sources. Deep Vent (exo-) (2 units/μL), Thermus Aquaticus (2 units/μL), and Bst DNA polymerase (8 units/μL), New England Biolabs: Superscript II RNase H (200 units/ μL), Invitrogen; DNA Sequenase (13 units/μL) and AMV reverse transcriptase (20 units/μL), Sigma; and HIV reverse transcriptase (27.3 units/μL), Worthington Biochemical Corp.
  • 15
    • 0012915759 scopus 로고    scopus 로고
    • note
    • Gel-purified DNA products were extended by 4-6 C residues by incubating with dCTP and terminal deoxynucleotidyl transferase (Promega). PCR amplification and subsequent digestion with EcoR1 (New England Biolabs) allowed individual sequences to be concatenated into multimers with T4 DNA ligase (New England Biolabs). Oligonucleotides were cloned into a TOPO vector (Invitrogen) and sequenced (MGH Molecular Biology Core Facility).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.