Development of a PCR-based assay for rapid and reliable identification of pathogenic Fusaria

FEMS Microbiology Letters

Volumn 218, Issue 2, 2003, Pages 329-332

  Mishra, Prashant K ^a,b   Fox, Roland T V ^a   Culham, Alastair ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Diagnosis; Fusarium; Identification; Internal transcribed spacer; Nuclear ribosomal DNA; Polymerase chain reaction

Indexed keywords

CARCINOGEN; DNA FRAGMENT; ETHIDIUM BROMIDE; FLUORESCENT DYE; FUNGAL DNA; OLIGONUCLEOTIDE; RIBOSOME DNA;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE; FUNGUS IDENTIFICATION; FUSARIUM; FUSARIUM AVENACEUM; FUSARIUM CULMORUM; FUSARIUM EQUISETI; FUSARIUM OXYSPORUM; FUSARIUM SAMBUCINUM; GEL ELECTROPHORESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BASE SEQUENCE; DNA PRIMERS; DNA, FUNGAL; DNA, RIBOSOMAL; FUSARIUM; MOLECULAR SEQUENCE DATA; MYCOSES; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; TIME FACTORS;

FUSARIA; FUSARIUM; FUSARIUM CULMORUM; FUSARIUM EQUISETI; FUSARIUM OXYSPORUM; GIBBERELLA AVENACEA;

EID: 0037469345     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)01174-6     Document Type: Article

References (18)

1. Desjardins A.E., Manandhar G.G., Plattner R.D., Maragos C.M., Shrestha K., McCormick S.P. Occurrence of Fusarium species and mycotoxins in Nepalese maize and wheat and the effect of traditional processing methods on mycotoxin levels. J. Agric. Food Chem. 48:2000;1377-1383.
2. Doohan F.M., Parry D.W., Jenkinson P., Nicholson P. The use of species-specific PCR based assays to analyse Fusarium ear blight of wheat. Plant Pathol. 47:1998;197-205.
3. Withanage G.S., Murata H., Koyama T., Ishiwata I. Agonistic and antagonistic effects of zearalenone, an estrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines. Vet. Hum. Toxicol. 43:2001;6-10.
4. McMullen M., Jones R., Gallenberg D. Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis. 81:1997;1340-1348.
5. Clear R.M., Patrick S.K., Gaba D. Prevalence of fungi and fusariotoxins on barley seed from western Canada, 1995 to 1997. Can. J. Plant Pathol. 22:2000;44-50.
6. Wollenweber, H.W. and Reinking, O.A. (1935) Die Fusarien. Ihre Beschreibung Schadwirkung and Bakampfung. Paul Parey, Berlin.
7. Snyder W.C., Hansen H.N. The species concept in Fusarium with reference to Discolor and other species. Am. J. Bot. 28:1945;738-742.
8. Booth, C. (1971) The Genus Fusarium. Commonwealth Mycological Institute, Kew.
9. Gerlach W., Nirenberg H.I. The genus Fusarium - A pictorial atlas. Mitt. Biol. Bundesanst Land-forstwirtsch. 209:1982;1-406.
10. Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O. (1983) Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park, PA.
11. Donaldson G.C., Ball L.A., Axelrood P.E., Glass N.L. Primer sets developed to amplify conserved genes from filamentous ascomycetes are useful in differentiating Fusarium species associated with conifers. Appl. Environ. Microbiol. 61:1995;1331-1340.
12. Taylor J.W., Geiser D.M., Burt A., Koufopanou V. The evolutionary biology and population genetics underlying fungal strain typing. Clin. Microbiol. Rev. 12:1999;126-146.
13. O'Donnell K., Cigelnik E., Casper H.H. Molecular phylogenetic, morphological and mycotoxin data support re-identification of the quorn mycoprotein fungus as Fusarium venenatum. Fungal Genet. Biol. 23:1998;57-67.
14. Lee, S.B. and Taylor, J.W. (1990) Isolation of DNA from fungal mycelia and single spores. In: PCR Protocols a Guide to Methods and Applications (Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., Eds.), pp. 282-314. Academic Press, New York.
15. White, T.J., Bruns, T., Lee, S.B. and Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: PCR Protocols a Guide to Methods and Applications (Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White, T.J., Eds.), pp. 315-322. Academic Press, New York.
16. O'Donnell K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia. 92:2000;919-938.
17. Yoder W.T., Christianson L.M. Species-specific primers resolve members of Fusarium section Fusarium. Fungal Genet. Biol. 23:1997;68-80.
18. Nicholson P., Lees A.K., Maurin N., Parry D.W., Rezanoor H.N. Development of a PCR assay to identify and quantify Microdochium nivale var. nivale and Microdochium nivale var. majus in wheat. Physiol. Mol. Plant Pathol. 48:1996;257-271.