Simple liquid-chromatographic method for Nile Red quantification in cell culture in spite of photobleaching

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 787, Issue 2, 2003, Pages 415-419

  Lamprecht, Alf ^a   Benoit, Jean Pierre ^a  

^a UNIVERSITÉ D'ANGERS   (France)

Author keywords

Nile Red; Photobleached samples

Indexed keywords

ASSAYS; CELLS; FLUORESCENCE; IMAGING SYSTEMS; ULTRAVIOLET RADIATION;

FLUORESCENT MARKER;

LIQUID CHROMATOGRAPHY;

DRUG CARRIER; DYE; NILE RED; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; BIOASSAY; BLEACHING; CELL CULTURE; CELL STRUCTURE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMAGING; PRIORITY JOURNAL; RISK FACTOR; STAINING; ULTRAVIOLET SPECTROSCOPY;

EID: 0037466203     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00962-5     Document Type: Article

References (13)

1. Kreuter J. Colloidal Drug Delivery Systems. Drugs and the Pharmaceutical Sciences. 1994;Marcel Dekker, New York.
2. Greenspan P., Mayer E.P., Fowler S.D. J. Cell Biol. 100:1985;965.
3. Fowler S.D., Greenspan P. J. Histochem. Cytochem. 33:1985;833.
4. Brown W.J., Sullivan T.R., Greenspan P. Histochemistry. 97:1992;349.
5. Ho C.H., Hlady V. Biomaterials. 16:1995;479.
6. Yoshida Y., Yoneda K., Umeda M., Ide C., Fujimoto K. Br. J. Dermatol. 145:2001;758.
7. Leroux J.C., Gravel P., Balant L., Volet B., Anner B.M., Allemann E., Doelker E., Gurny R. J. Biomed. Mater. Res. 28:1994;471.
8. Gessner A., Olbrich C., Schroder W., Kayser O., Müller R.H. Int. J. Pharm. 214:2001;87.
9. Ogiso T., Yamaguchi T., Iwaki M., Tanino T., Miyake Y. J. Drug Target. 9:2001;49.
10. Ogiso T., Niinaka N., Iwaki M. J. Pharm. Sci. 85:1996;57.
11. Greenspan P., Fowler S.D. J. Lipid Res. 26:1985;781.
12. Veiro J.A., Cummins P.G. Dermatology. 189:1994;16.
13. Lehr C.M. Cell culture Models of Biological Barriers. 2002;Taylor and Francis, London.